Basolateral junction proteins regulate competition for the follicle stem cell niche in the Drosophila ovary.

Epithelial stem cells are routinely lost or damaged during adult life and must therefore be replaced to maintain homeostasis. Recent studies indicate that stem cell replacement occurs through neutral competition in many types of epithelial tissues, but little is known about the factors that determin...

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Autores principales:	Maria R Kronen, Kevin P Schoenfelder, Allon M Klein, Todd G Nystul
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ebb09ea4fdd34cc2b02ba992e590437d
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spelling	oai:doaj.org-article:ebb09ea4fdd34cc2b02ba992e590437d2021-11-25T06:09:49ZBasolateral junction proteins regulate competition for the follicle stem cell niche in the Drosophila ovary.1932-620310.1371/journal.pone.0101085https://doaj.org/article/ebb09ea4fdd34cc2b02ba992e590437d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24991805/?tool=EBIhttps://doaj.org/toc/1932-6203Epithelial stem cells are routinely lost or damaged during adult life and must therefore be replaced to maintain homeostasis. Recent studies indicate that stem cell replacement occurs through neutral competition in many types of epithelial tissues, but little is known about the factors that determine competitive outcome. The epithelial follicle stem cells (FSCs) in the Drosophila ovary are regularly lost and replaced during normal homeostasis, and we show that FSC replacement conforms to a model of neutral competition. In addition, we found that FSCs mutant for the basolateral junction genes, lethal giant larvae (lgl) or discs large (dlg), undergo a biased competition for niche occupancy characterized by increased invasion of neighboring FSCs and reduced loss. Interestingly, FSCs mutant for a third basolateral junction gene, scribble (scrib), do not exhibit biased competition, suggesting that Lgl and Dlg regulate niche competition through a Scrib-independent process. Lastly, we found that FSCs have a unique cell polarity characterized by broadly distributed adherens junctions and the lack of a mature apical domain. Collectively, these observations indicate that Lgl and Dlg promote the differentiation of FSC progeny to a state in which they are less prone to invade the neighboring niche. In addition, we demonstrate that the neutral drift model can be adapted to quantify non-neutral behavior of mutant clones.Maria R KronenKevin P SchoenfelderAllon M KleinTodd G NystulPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e101085 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria R Kronen Kevin P Schoenfelder Allon M Klein Todd G Nystul Basolateral junction proteins regulate competition for the follicle stem cell niche in the Drosophila ovary.
description	Epithelial stem cells are routinely lost or damaged during adult life and must therefore be replaced to maintain homeostasis. Recent studies indicate that stem cell replacement occurs through neutral competition in many types of epithelial tissues, but little is known about the factors that determine competitive outcome. The epithelial follicle stem cells (FSCs) in the Drosophila ovary are regularly lost and replaced during normal homeostasis, and we show that FSC replacement conforms to a model of neutral competition. In addition, we found that FSCs mutant for the basolateral junction genes, lethal giant larvae (lgl) or discs large (dlg), undergo a biased competition for niche occupancy characterized by increased invasion of neighboring FSCs and reduced loss. Interestingly, FSCs mutant for a third basolateral junction gene, scribble (scrib), do not exhibit biased competition, suggesting that Lgl and Dlg regulate niche competition through a Scrib-independent process. Lastly, we found that FSCs have a unique cell polarity characterized by broadly distributed adherens junctions and the lack of a mature apical domain. Collectively, these observations indicate that Lgl and Dlg promote the differentiation of FSC progeny to a state in which they are less prone to invade the neighboring niche. In addition, we demonstrate that the neutral drift model can be adapted to quantify non-neutral behavior of mutant clones.
format	article
author	Maria R Kronen Kevin P Schoenfelder Allon M Klein Todd G Nystul
author_facet	Maria R Kronen Kevin P Schoenfelder Allon M Klein Todd G Nystul
author_sort	Maria R Kronen
title	Basolateral junction proteins regulate competition for the follicle stem cell niche in the Drosophila ovary.
title_short	Basolateral junction proteins regulate competition for the follicle stem cell niche in the Drosophila ovary.
title_full	Basolateral junction proteins regulate competition for the follicle stem cell niche in the Drosophila ovary.
title_fullStr	Basolateral junction proteins regulate competition for the follicle stem cell niche in the Drosophila ovary.
title_full_unstemmed	Basolateral junction proteins regulate competition for the follicle stem cell niche in the Drosophila ovary.
title_sort	basolateral junction proteins regulate competition for the follicle stem cell niche in the drosophila ovary.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ebb09ea4fdd34cc2b02ba992e590437d
work_keys_str_mv	AT mariarkronen basolateraljunctionproteinsregulatecompetitionforthefolliclestemcellnicheinthedrosophilaovary AT kevinpschoenfelder basolateraljunctionproteinsregulatecompetitionforthefolliclestemcellnicheinthedrosophilaovary AT allonmklein basolateraljunctionproteinsregulatecompetitionforthefolliclestemcellnicheinthedrosophilaovary AT toddgnystul basolateraljunctionproteinsregulatecompetitionforthefolliclestemcellnicheinthedrosophilaovary
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Basolateral junction proteins regulate competition for the follicle stem cell niche in the Drosophila ovary.

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