Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.

Hypertension and congenital aortic valve malformations are frequent causes of ascending aortic aneurysms. The molecular mechanisms of aneurysm formation under these circumstances are not well understood. Reference genes for gene activity studies in aortic tissue that are not influenced by aortic val...

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Autores principales:	Dominic Henn, Doris Bandner-Risch, Hilja Perttunen, Wolfram Schmied, Carlos Porras, Francisco Ceballos, Noela Rodriguez-Losada, Hans-Joachim Schäfers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ebb2527ce32347fcbb598f742b49245e
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spelling	oai:doaj.org-article:ebb2527ce32347fcbb598f742b49245e2021-11-18T08:01:45ZIdentification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.1932-620310.1371/journal.pone.0054132https://doaj.org/article/ebb2527ce32347fcbb598f742b49245e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23326585/?tool=EBIhttps://doaj.org/toc/1932-6203Hypertension and congenital aortic valve malformations are frequent causes of ascending aortic aneurysms. The molecular mechanisms of aneurysm formation under these circumstances are not well understood. Reference genes for gene activity studies in aortic tissue that are not influenced by aortic valve morphology and its hemodynamic consequences, aortic dilatation, hypertension, or antihypertensive medication are not available so far. This study determines genes in ascending aortic tissue that are independent of these parameters. Tissue specimens from dilated and undilated ascending aortas were obtained from 60 patients (age ≤70 years) with different morphologies of the aortic valve (tricuspid undilated n = 24, dilated n = 11; bicuspid undilated n = 6, dilated n = 15; unicuspid dilated n = 4). Of the studied individuals, 36 had hypertension, and 31 received ACE inhibitors or AT1 receptor antagonists. The specimens were obtained intraoperatively from the wall of the ascending aorta. We analyzed the expression levels of 32 candidate reference genes by quantitative RT-PCR (RT-qPCR). Differential expression levels were assessed by parametric statistics. The expression analysis of these 32 genes by RT-qPCR showed that EIF2B1, ELF1, and PPIA remained constant in their expression levels in the different specimen groups, thus being insensitive to aortic valve morphology, aortic dilatation, hypertension, and medication with ACE inhibitors or AT1 receptor antagonists. Unlike many other commonly used reference genes, the genes EIF2B1, ELF1, and PPIA are neither confounded by aortic comorbidities nor by antihypertensive medication and therefore are most suitable for gene expression analysis of ascending aortic tissue.Dominic HennDoris Bandner-RischHilja PerttunenWolfram SchmiedCarlos PorrasFrancisco CeballosNoela Rodriguez-LosadaHans-Joachim SchäfersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54132 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dominic Henn Doris Bandner-Risch Hilja Perttunen Wolfram Schmied Carlos Porras Francisco Ceballos Noela Rodriguez-Losada Hans-Joachim Schäfers Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.
description	Hypertension and congenital aortic valve malformations are frequent causes of ascending aortic aneurysms. The molecular mechanisms of aneurysm formation under these circumstances are not well understood. Reference genes for gene activity studies in aortic tissue that are not influenced by aortic valve morphology and its hemodynamic consequences, aortic dilatation, hypertension, or antihypertensive medication are not available so far. This study determines genes in ascending aortic tissue that are independent of these parameters. Tissue specimens from dilated and undilated ascending aortas were obtained from 60 patients (age ≤70 years) with different morphologies of the aortic valve (tricuspid undilated n = 24, dilated n = 11; bicuspid undilated n = 6, dilated n = 15; unicuspid dilated n = 4). Of the studied individuals, 36 had hypertension, and 31 received ACE inhibitors or AT1 receptor antagonists. The specimens were obtained intraoperatively from the wall of the ascending aorta. We analyzed the expression levels of 32 candidate reference genes by quantitative RT-PCR (RT-qPCR). Differential expression levels were assessed by parametric statistics. The expression analysis of these 32 genes by RT-qPCR showed that EIF2B1, ELF1, and PPIA remained constant in their expression levels in the different specimen groups, thus being insensitive to aortic valve morphology, aortic dilatation, hypertension, and medication with ACE inhibitors or AT1 receptor antagonists. Unlike many other commonly used reference genes, the genes EIF2B1, ELF1, and PPIA are neither confounded by aortic comorbidities nor by antihypertensive medication and therefore are most suitable for gene expression analysis of ascending aortic tissue.
format	article
author	Dominic Henn Doris Bandner-Risch Hilja Perttunen Wolfram Schmied Carlos Porras Francisco Ceballos Noela Rodriguez-Losada Hans-Joachim Schäfers
author_facet	Dominic Henn Doris Bandner-Risch Hilja Perttunen Wolfram Schmied Carlos Porras Francisco Ceballos Noela Rodriguez-Losada Hans-Joachim Schäfers
author_sort	Dominic Henn
title	Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.
title_short	Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.
title_full	Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.
title_fullStr	Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.
title_full_unstemmed	Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.
title_sort	identification of reference genes for quantitative rt-pcr in ascending aortic aneurysms.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ebb2527ce32347fcbb598f742b49245e
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Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms.

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