Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production

Abstract Recent evidence supports involvement of the acute phase protein haptoglobin in numerous events during mammalian reproduction. The present study represents an in-depth investigation of haptoglobin expression and secretion in the porcine oviduct and uterus, and assesses its effect on porcine...

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Autores principales:	Francisco A. García-Vázquez, Carla Moros-Nicolás, Rebeca López-Úbeda, Ernesto Rodríguez-Tobón, Ascensión Guillén-Martínez, Jason W. Ross, Chiara Luongo, Carmen Matás, Iván Hernández-Caravaca, Manuel Avilés, Mª José Izquierdo-Rico
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ebb56bf04fd94be5834846ad86890e7f
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spelling	oai:doaj.org-article:ebb56bf04fd94be5834846ad86890e7f2021-12-02T15:02:51ZEvidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production10.1038/s41598-021-90810-62045-2322https://doaj.org/article/ebb56bf04fd94be5834846ad86890e7f2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90810-6https://doaj.org/toc/2045-2322Abstract Recent evidence supports involvement of the acute phase protein haptoglobin in numerous events during mammalian reproduction. The present study represents an in-depth investigation of haptoglobin expression and secretion in the porcine oviduct and uterus, and assesses its effect on porcine in vitro embryo production. A systematic study was made of sows in different oestrous stages: late follicular, early luteal and late luteal stages. Relative haptoglobin mRNA abundance was quantified by RT-qPCR. In addition, expression of the protein was analysed by immunohistochemistry and the results were complemented by Western-blot and proteomic analyses of the oviductal and uterine fluids. In vitro porcine fertilization and embryo culture were carried out in the presence of haptoglobin. The results indicate that haptoglobin mRNA expression in the porcine oviduct and uterus is most abundant during the late luteal stage of the oestrous cycle. By means of Western blot and proteomic analyses haptoglobin presence was demonstrated in the oviduct epithelium and in the oviductal and uterine fluids in different stages of the oestrous cycle. The addition of haptoglobin during gamete co-incubation had no effect on sperm penetration, monospermy or efficiency rates; however, compared with the control group, blastocyst development was significantly improved when haptoglobin was present (haptoglobin: 64.50% vs. control: 37.83%; p < 0.05). In conclusion, the presence of haptoglobin in the oviduct and uterus of sows at different stages of the oestrous cycle suggests that it plays an important role in the reproduction process. The addition of haptoglobin during in vitro embryo production improved the blastocyst rates.Francisco A. García-VázquezCarla Moros-NicolásRebeca López-ÚbedaErnesto Rodríguez-TobónAscensión Guillén-MartínezJason W. RossChiara LuongoCarmen MatásIván Hernández-CaravacaManuel AvilésMª José Izquierdo-RicoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francisco A. García-Vázquez Carla Moros-Nicolás Rebeca López-Úbeda Ernesto Rodríguez-Tobón Ascensión Guillén-Martínez Jason W. Ross Chiara Luongo Carmen Matás Iván Hernández-Caravaca Manuel Avilés Mª José Izquierdo-Rico Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production
description	Abstract Recent evidence supports involvement of the acute phase protein haptoglobin in numerous events during mammalian reproduction. The present study represents an in-depth investigation of haptoglobin expression and secretion in the porcine oviduct and uterus, and assesses its effect on porcine in vitro embryo production. A systematic study was made of sows in different oestrous stages: late follicular, early luteal and late luteal stages. Relative haptoglobin mRNA abundance was quantified by RT-qPCR. In addition, expression of the protein was analysed by immunohistochemistry and the results were complemented by Western-blot and proteomic analyses of the oviductal and uterine fluids. In vitro porcine fertilization and embryo culture were carried out in the presence of haptoglobin. The results indicate that haptoglobin mRNA expression in the porcine oviduct and uterus is most abundant during the late luteal stage of the oestrous cycle. By means of Western blot and proteomic analyses haptoglobin presence was demonstrated in the oviduct epithelium and in the oviductal and uterine fluids in different stages of the oestrous cycle. The addition of haptoglobin during gamete co-incubation had no effect on sperm penetration, monospermy or efficiency rates; however, compared with the control group, blastocyst development was significantly improved when haptoglobin was present (haptoglobin: 64.50% vs. control: 37.83%; p < 0.05). In conclusion, the presence of haptoglobin in the oviduct and uterus of sows at different stages of the oestrous cycle suggests that it plays an important role in the reproduction process. The addition of haptoglobin during in vitro embryo production improved the blastocyst rates.
format	article
author	Francisco A. García-Vázquez Carla Moros-Nicolás Rebeca López-Úbeda Ernesto Rodríguez-Tobón Ascensión Guillén-Martínez Jason W. Ross Chiara Luongo Carmen Matás Iván Hernández-Caravaca Manuel Avilés Mª José Izquierdo-Rico
author_facet	Francisco A. García-Vázquez Carla Moros-Nicolás Rebeca López-Úbeda Ernesto Rodríguez-Tobón Ascensión Guillén-Martínez Jason W. Ross Chiara Luongo Carmen Matás Iván Hernández-Caravaca Manuel Avilés Mª José Izquierdo-Rico
author_sort	Francisco A. García-Vázquez
title	Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production
title_short	Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production
title_full	Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production
title_fullStr	Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production
title_full_unstemmed	Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production
title_sort	evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ebb56bf04fd94be5834846ad86890e7f
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Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production

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