Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss

Several studies have shown that type IV fibrocytes, located in the spiral ligament, degenerate first after noise exposure. Interestingly, this is the region where <i>Coch</i> expression is most abundant. As it is suggested that cochlin plays a role in our innate immune system, our goal i...

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Autores principales:	Dorien Verdoodt, Noa Peeleman, Krystyna Szewczyk, Guy Van Camp, Peter Ponsaerts, Vincent Van Rompaey
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	noise exposure inner ear inflammation <i>Coch</i> knockout spiral ligament Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/ebb5cd1cf8f14c7db193894653ccae22
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id	oai:doaj.org-article:ebb5cd1cf8f14c7db193894653ccae22
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spelling	oai:doaj.org-article:ebb5cd1cf8f14c7db193894653ccae222021-11-11T17:01:26ZCochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss10.3390/ijms2221115491422-00671661-6596https://doaj.org/article/ebb5cd1cf8f14c7db193894653ccae222021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11549https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Several studies have shown that type IV fibrocytes, located in the spiral ligament, degenerate first after noise exposure. Interestingly, this is the region where <i>Coch</i> expression is most abundant. As it is suggested that cochlin plays a role in our innate immune system, our goal is to investigate hearing thresholds and inner ear inflammation after noise exposure in <i>Coch</i> knockout (<i>Coch<sup>−/−</sup></i>) mice compared to <i>Coch</i> wildtype (<i>Coch<sup>+/+</sup></i>) mice. Animals were randomly allocated to a noise exposure group and a control group. Vestibular and auditory testing was performed at 48 h and one week after noise exposure. Whole mount staining and cryosectioning of the cochlea was performed in order to investigate hair cells, spiral ganglion neurons, inner ear inflammation, <i>Coch</i> expression and fibrocyte degeneration. Hearing assessment revealed that <i>Coch<sup>+/+</sup></i> mice had significantly larger threshold shifts than <i>Coch<sup>−/−</sup></i> mice after noise exposure. We were unable to identify any differences in hair cells, neurons, fibrocytes and influx of macrophages in the inner ear between both groups. Interestingly, <i>Coch</i> expression was significantly lower in the group exposed to noise. Our results indicate that the absence of <i>Coch</i> has a protective influence on hearing thresholds after noise exposure, but this is not related to reduced inner ear inflammation in the knockout.Dorien VerdoodtNoa PeelemanKrystyna SzewczykGuy Van CampPeter PonsaertsVincent Van RompaeyMDPI AGarticlenoise exposureinner ear inflammation<i>Coch</i> knockoutspiral ligamentBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11549, p 11549 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	noise exposure inner ear inflammation <i>Coch</i> knockout spiral ligament Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	noise exposure inner ear inflammation <i>Coch</i> knockout spiral ligament Biology (General) QH301-705.5 Chemistry QD1-999 Dorien Verdoodt Noa Peeleman Krystyna Szewczyk Guy Van Camp Peter Ponsaerts Vincent Van Rompaey Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss
description	Several studies have shown that type IV fibrocytes, located in the spiral ligament, degenerate first after noise exposure. Interestingly, this is the region where <i>Coch</i> expression is most abundant. As it is suggested that cochlin plays a role in our innate immune system, our goal is to investigate hearing thresholds and inner ear inflammation after noise exposure in <i>Coch</i> knockout (<i>Coch<sup>−/−</sup></i>) mice compared to <i>Coch</i> wildtype (<i>Coch<sup>+/+</sup></i>) mice. Animals were randomly allocated to a noise exposure group and a control group. Vestibular and auditory testing was performed at 48 h and one week after noise exposure. Whole mount staining and cryosectioning of the cochlea was performed in order to investigate hair cells, spiral ganglion neurons, inner ear inflammation, <i>Coch</i> expression and fibrocyte degeneration. Hearing assessment revealed that <i>Coch<sup>+/+</sup></i> mice had significantly larger threshold shifts than <i>Coch<sup>−/−</sup></i> mice after noise exposure. We were unable to identify any differences in hair cells, neurons, fibrocytes and influx of macrophages in the inner ear between both groups. Interestingly, <i>Coch</i> expression was significantly lower in the group exposed to noise. Our results indicate that the absence of <i>Coch</i> has a protective influence on hearing thresholds after noise exposure, but this is not related to reduced inner ear inflammation in the knockout.
format	article
author	Dorien Verdoodt Noa Peeleman Krystyna Szewczyk Guy Van Camp Peter Ponsaerts Vincent Van Rompaey
author_facet	Dorien Verdoodt Noa Peeleman Krystyna Szewczyk Guy Van Camp Peter Ponsaerts Vincent Van Rompaey
author_sort	Dorien Verdoodt
title	Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss
title_short	Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss
title_full	Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss
title_fullStr	Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss
title_full_unstemmed	Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss
title_sort	cochlin deficiency protects aged mice from noise-induced hearing loss
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ebb5cd1cf8f14c7db193894653ccae22
work_keys_str_mv	AT dorienverdoodt cochlindeficiencyprotectsagedmicefromnoiseinducedhearingloss AT noapeeleman cochlindeficiencyprotectsagedmicefromnoiseinducedhearingloss AT krystynaszewczyk cochlindeficiencyprotectsagedmicefromnoiseinducedhearingloss AT guyvancamp cochlindeficiencyprotectsagedmicefromnoiseinducedhearingloss AT peterponsaerts cochlindeficiencyprotectsagedmicefromnoiseinducedhearingloss AT vincentvanrompaey cochlindeficiencyprotectsagedmicefromnoiseinducedhearingloss
_version_	1718432210649874432

Cochlin Deficiency Protects Aged Mice from Noise-Induced Hearing Loss

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