EGCG promotes PRKCA expression to alleviate LPS-induced acute lung injury and inflammatory response

Abstract Acute lung injury (ALI), which could be induced by multiple factors such as lipopolysaccharide (LPS), refer to clinical symptoms of acute respiratory failure, commonly with high morbidity and mortality. Reportedly, active ingredients from green tea have anti-inflammatory and anticancer prop...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mian Wang, Hua Zhong, Xian Zhang, Xin Huang, Jing Wang, Zihao Li, Mengshi Chen, Zhenghui Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ebbaa7305f0741cfa6ad2a7d9ac81215
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ebbaa7305f0741cfa6ad2a7d9ac81215
record_format	dspace
spelling	oai:doaj.org-article:ebbaa7305f0741cfa6ad2a7d9ac812152021-12-02T15:49:31ZEGCG promotes PRKCA expression to alleviate LPS-induced acute lung injury and inflammatory response10.1038/s41598-021-90398-x2045-2322https://doaj.org/article/ebbaa7305f0741cfa6ad2a7d9ac812152021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90398-xhttps://doaj.org/toc/2045-2322Abstract Acute lung injury (ALI), which could be induced by multiple factors such as lipopolysaccharide (LPS), refer to clinical symptoms of acute respiratory failure, commonly with high morbidity and mortality. Reportedly, active ingredients from green tea have anti-inflammatory and anticancer properties, including epigallocatechin-3-gallate (EGCG). In the present study, protein kinase C alpha (PRKCA) is involved in EGCG protection against LPS-induced inflammation and ALI. EGCG treatment attenuated LPS-stimulated ALI in mice as manifested as improved lung injury scores, decreased total cell amounts, neutrophil amounts and macrophage amounts, inhibited the activity of MPO, decreased wet-to-dry weight ratio of lung tissues, and inhibited release of inflammatory cytokines TNF-α, IL-1β, and IL-6. PRKCA mRNA and protein expression showed to be dramatically decreased by LPS treatment while reversed by EGCG treatment. Within LPS-stimulated ALI mice, PRKCA silencing further aggravated, while PRKCA overexpression attenuated LPS-stimulated inflammation and ALI through MAPK signaling pathway. PRKCA silencing attenuated EGCG protection. Within LPS-induced RAW 264.7 macrophages, EGCG could induce PRKCA expression. Single EGCG treatment or Lv-PRKCA infection attenuated LPS-induced increases in inflammatory factors; PRKCA silencing could reverse the suppressive effects of EGCG upon LPS-stimulated inflammatory factor release. In conclusion, EGCG pretreatment inhibits LPS-induced ALI in mice. The protective mechanism might be associated with the inhibitory effects of PRKCA on proinflammatory cytokine release via macrophages and MAPK signaling pathway.Mian WangHua ZhongXian ZhangXin HuangJing WangZihao LiMengshi ChenZhenghui XiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mian Wang Hua Zhong Xian Zhang Xin Huang Jing Wang Zihao Li Mengshi Chen Zhenghui Xiao EGCG promotes PRKCA expression to alleviate LPS-induced acute lung injury and inflammatory response
description	Abstract Acute lung injury (ALI), which could be induced by multiple factors such as lipopolysaccharide (LPS), refer to clinical symptoms of acute respiratory failure, commonly with high morbidity and mortality. Reportedly, active ingredients from green tea have anti-inflammatory and anticancer properties, including epigallocatechin-3-gallate (EGCG). In the present study, protein kinase C alpha (PRKCA) is involved in EGCG protection against LPS-induced inflammation and ALI. EGCG treatment attenuated LPS-stimulated ALI in mice as manifested as improved lung injury scores, decreased total cell amounts, neutrophil amounts and macrophage amounts, inhibited the activity of MPO, decreased wet-to-dry weight ratio of lung tissues, and inhibited release of inflammatory cytokines TNF-α, IL-1β, and IL-6. PRKCA mRNA and protein expression showed to be dramatically decreased by LPS treatment while reversed by EGCG treatment. Within LPS-stimulated ALI mice, PRKCA silencing further aggravated, while PRKCA overexpression attenuated LPS-stimulated inflammation and ALI through MAPK signaling pathway. PRKCA silencing attenuated EGCG protection. Within LPS-induced RAW 264.7 macrophages, EGCG could induce PRKCA expression. Single EGCG treatment or Lv-PRKCA infection attenuated LPS-induced increases in inflammatory factors; PRKCA silencing could reverse the suppressive effects of EGCG upon LPS-stimulated inflammatory factor release. In conclusion, EGCG pretreatment inhibits LPS-induced ALI in mice. The protective mechanism might be associated with the inhibitory effects of PRKCA on proinflammatory cytokine release via macrophages and MAPK signaling pathway.
format	article
author	Mian Wang Hua Zhong Xian Zhang Xin Huang Jing Wang Zihao Li Mengshi Chen Zhenghui Xiao
author_facet	Mian Wang Hua Zhong Xian Zhang Xin Huang Jing Wang Zihao Li Mengshi Chen Zhenghui Xiao
author_sort	Mian Wang
title	EGCG promotes PRKCA expression to alleviate LPS-induced acute lung injury and inflammatory response
title_short	EGCG promotes PRKCA expression to alleviate LPS-induced acute lung injury and inflammatory response
title_full	EGCG promotes PRKCA expression to alleviate LPS-induced acute lung injury and inflammatory response
title_fullStr	EGCG promotes PRKCA expression to alleviate LPS-induced acute lung injury and inflammatory response
title_full_unstemmed	EGCG promotes PRKCA expression to alleviate LPS-induced acute lung injury and inflammatory response
title_sort	egcg promotes prkca expression to alleviate lps-induced acute lung injury and inflammatory response
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ebbaa7305f0741cfa6ad2a7d9ac81215
work_keys_str_mv	AT mianwang egcgpromotesprkcaexpressiontoalleviatelpsinducedacutelunginjuryandinflammatoryresponse AT huazhong egcgpromotesprkcaexpressiontoalleviatelpsinducedacutelunginjuryandinflammatoryresponse AT xianzhang egcgpromotesprkcaexpressiontoalleviatelpsinducedacutelunginjuryandinflammatoryresponse AT xinhuang egcgpromotesprkcaexpressiontoalleviatelpsinducedacutelunginjuryandinflammatoryresponse AT jingwang egcgpromotesprkcaexpressiontoalleviatelpsinducedacutelunginjuryandinflammatoryresponse AT zihaoli egcgpromotesprkcaexpressiontoalleviatelpsinducedacutelunginjuryandinflammatoryresponse AT mengshichen egcgpromotesprkcaexpressiontoalleviatelpsinducedacutelunginjuryandinflammatoryresponse AT zhenghuixiao egcgpromotesprkcaexpressiontoalleviatelpsinducedacutelunginjuryandinflammatoryresponse
_version_	1718385724590391296

EGCG promotes PRKCA expression to alleviate LPS-induced acute lung injury and inflammatory response

Ejemplares similares