Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)

Drought is the main abiotic stress factor limiting the growth and yield of wheat (Triticum aestivum L.). Therefore, improving wheat tolerance to drought stress is essential for maintaining yield. Previous studies have reported on the important role of TaNRX1 in conferring drought stress tolerance. T...

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Autores principales:	Yunrui Zhang, Jianfei Zhou, Fan Wei, Tianqi Song, Yang Yu, Ming Yu, Qiru Fan, Yanning Yang, Gang Xue, Xiaoke Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	wheat nucleoredoxin TaNRX1 drought RNA-seq Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/ebc1b33a04174c2fb4ce283b3beb86c2
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spelling	oai:doaj.org-article:ebc1b33a04174c2fb4ce283b3beb86c22021-11-11T13:57:47ZNucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)1664-462X10.3389/fpls.2021.756338https://doaj.org/article/ebc1b33a04174c2fb4ce283b3beb86c22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.756338/fullhttps://doaj.org/toc/1664-462XDrought is the main abiotic stress factor limiting the growth and yield of wheat (Triticum aestivum L.). Therefore, improving wheat tolerance to drought stress is essential for maintaining yield. Previous studies have reported on the important role of TaNRX1 in conferring drought stress tolerance. Therefore, to elucidate the regulation mechanism by which TaNRX1 confers drought resistance in wheat, we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines. The results showed that the tolerance of the OE lines to drought stress were significantly enhanced. The survival rate, leaf chlorophyll, proline, soluble sugar content, and activities of the antioxidant enzymes (catalase, superoxide dismutase, and peroxidase) of the OE lines were higher than those of the wild type (WT); however, the relative electrical conductivity and malondialdehyde, hydrogen peroxide, and superoxide anion levels of the OE lines were lower than those of the WT; the RNAi lines showed the opposite results. RNA-seq results showed that the common differentially expressed genes of TaNRX1 OE and RNAi lines, before and after drought stress, were mainly distributed in the plant–pathogen interaction, plant hormone signal transduction, phenylpropane biosynthesis, starch and sucrose metabolism, and carbon metabolism pathways and were related to the transcription factors, including WRKY, MYB, and bHLH families. This study suggests that TaNRX1 positively regulates drought stress tolerance in wheat.Yunrui ZhangJianfei ZhouFan WeiTianqi SongYang YuMing YuQiru FanYanning YangGang XueXiaoke ZhangFrontiers Media S.A.articlewheatnucleoredoxinTaNRX1droughtRNA-seqPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	wheat nucleoredoxin TaNRX1 drought RNA-seq Plant culture SB1-1110
spellingShingle	wheat nucleoredoxin TaNRX1 drought RNA-seq Plant culture SB1-1110 Yunrui Zhang Jianfei Zhou Fan Wei Tianqi Song Yang Yu Ming Yu Qiru Fan Yanning Yang Gang Xue Xiaoke Zhang Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)
description	Drought is the main abiotic stress factor limiting the growth and yield of wheat (Triticum aestivum L.). Therefore, improving wheat tolerance to drought stress is essential for maintaining yield. Previous studies have reported on the important role of TaNRX1 in conferring drought stress tolerance. Therefore, to elucidate the regulation mechanism by which TaNRX1 confers drought resistance in wheat, we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines. The results showed that the tolerance of the OE lines to drought stress were significantly enhanced. The survival rate, leaf chlorophyll, proline, soluble sugar content, and activities of the antioxidant enzymes (catalase, superoxide dismutase, and peroxidase) of the OE lines were higher than those of the wild type (WT); however, the relative electrical conductivity and malondialdehyde, hydrogen peroxide, and superoxide anion levels of the OE lines were lower than those of the WT; the RNAi lines showed the opposite results. RNA-seq results showed that the common differentially expressed genes of TaNRX1 OE and RNAi lines, before and after drought stress, were mainly distributed in the plant–pathogen interaction, plant hormone signal transduction, phenylpropane biosynthesis, starch and sucrose metabolism, and carbon metabolism pathways and were related to the transcription factors, including WRKY, MYB, and bHLH families. This study suggests that TaNRX1 positively regulates drought stress tolerance in wheat.
format	article
author	Yunrui Zhang Jianfei Zhou Fan Wei Tianqi Song Yang Yu Ming Yu Qiru Fan Yanning Yang Gang Xue Xiaoke Zhang
author_facet	Yunrui Zhang Jianfei Zhou Fan Wei Tianqi Song Yang Yu Ming Yu Qiru Fan Yanning Yang Gang Xue Xiaoke Zhang
author_sort	Yunrui Zhang
title	Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)
title_short	Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)
title_full	Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)
title_fullStr	Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)
title_full_unstemmed	Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)
title_sort	nucleoredoxin gene tanrx1 positively regulates drought tolerance in transgenic wheat (triticum aestivum l.)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ebc1b33a04174c2fb4ce283b3beb86c2
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Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)

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