A novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS437

Abstract Bacteriophages constitute key gene transfer agents in many bacteria. Specifically, they may confer gene mobility to Paraburkholderia spp. that dwells in soil and the mycosphere. In this study, we first screened mycosphere and bulk soils for phages able to produce plaques, however found thes...

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Autores principales:	Akbar Adjie Pratama, Jan Dirk van Elsas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ebc849d00caa4fafbb67eaeb62c0b5ef
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spelling	oai:doaj.org-article:ebc849d00caa4fafbb67eaeb62c0b5ef2021-12-02T16:06:38ZA novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS43710.1038/s41598-017-09317-82045-2322https://doaj.org/article/ebc849d00caa4fafbb67eaeb62c0b5ef2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09317-8https://doaj.org/toc/2045-2322Abstract Bacteriophages constitute key gene transfer agents in many bacteria. Specifically, they may confer gene mobility to Paraburkholderia spp. that dwells in soil and the mycosphere. In this study, we first screened mycosphere and bulk soils for phages able to produce plaques, however found these to be below detection. Then, prophage identification methods were applied to the genome sequences of the mycosphere-derived Paraburkholderia terrae strains BS001, BS007, BS110 and BS437, next to P. phytofirmans strains BS455, BIFAS53, J1U5 and PsJN. These analyses revealed all bacterial genomes to contain considerable amounts [up to 13.3%] of prophage-like sequences. One sequence predicted to encode a complete phage was found in the genome of P. terrae BS437. Using the inducing agent mitomycin C, we produced high-titered phage suspensions. These indeed encompassed the progeny of the identified prophage (denoted ɸ437), as evidenced using phage major capsid gene molecular detection. We obtained the full sequence of phage ɸ437, which, remarkably, had undergone a reshuffling of two large gene blocks. One predicted moron gene was found, and it is currently analyzed to understand the extent of its ecological significance for the host.Akbar Adjie PratamaJan Dirk van ElsasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akbar Adjie Pratama Jan Dirk van Elsas A novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS437
description	Abstract Bacteriophages constitute key gene transfer agents in many bacteria. Specifically, they may confer gene mobility to Paraburkholderia spp. that dwells in soil and the mycosphere. In this study, we first screened mycosphere and bulk soils for phages able to produce plaques, however found these to be below detection. Then, prophage identification methods were applied to the genome sequences of the mycosphere-derived Paraburkholderia terrae strains BS001, BS007, BS110 and BS437, next to P. phytofirmans strains BS455, BIFAS53, J1U5 and PsJN. These analyses revealed all bacterial genomes to contain considerable amounts [up to 13.3%] of prophage-like sequences. One sequence predicted to encode a complete phage was found in the genome of P. terrae BS437. Using the inducing agent mitomycin C, we produced high-titered phage suspensions. These indeed encompassed the progeny of the identified prophage (denoted ɸ437), as evidenced using phage major capsid gene molecular detection. We obtained the full sequence of phage ɸ437, which, remarkably, had undergone a reshuffling of two large gene blocks. One predicted moron gene was found, and it is currently analyzed to understand the extent of its ecological significance for the host.
format	article
author	Akbar Adjie Pratama Jan Dirk van Elsas
author_facet	Akbar Adjie Pratama Jan Dirk van Elsas
author_sort	Akbar Adjie Pratama
title	A novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS437
title_short	A novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS437
title_full	A novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS437
title_fullStr	A novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS437
title_full_unstemmed	A novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS437
title_sort	novel inducible prophage from the mycosphere inhabitant paraburkholderia terrae bs437
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ebc849d00caa4fafbb67eaeb62c0b5ef
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A novel inducible prophage from the mycosphere inhabitant Paraburkholderia terrae BS437

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