Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus

Abstract Staphylococcus aureus is the cause of serious vascular infections such as sepsis and endocarditis. These infections are notoriously difficult to treat, and it is believed that the ability of S. aureus to invade endothelial cells and persist intracellularly is a key mechanism for persistence...

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Autores principales:	Rasmus Birkholm Grønnemose, Christian Garde, Claes Søndergaard Wassmann, Janne Kudsk Klitgaard, Ronni Nielsen, Susanne Mandrup, Andreas Holm Mattsson, Thomas Emil Andersen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ebcbb48b41e24b86a95acf5c2d1df2bc
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spelling	oai:doaj.org-article:ebcbb48b41e24b86a95acf5c2d1df2bc2021-12-02T13:18:08ZBacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus10.1038/s41598-021-84050-x2045-2322https://doaj.org/article/ebcbb48b41e24b86a95acf5c2d1df2bc2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84050-xhttps://doaj.org/toc/2045-2322Abstract Staphylococcus aureus is the cause of serious vascular infections such as sepsis and endocarditis. These infections are notoriously difficult to treat, and it is believed that the ability of S. aureus to invade endothelial cells and persist intracellularly is a key mechanism for persistence despite ongoing antibiotic treatment. Here, we used dual RNA sequencing to study the simultaneous transcriptional response of S. aureus and human endothelial cells during in vitro infections. We revealed discrete and shared differentially expressed genes for both host and pathogen at the different stages of infection. While the endothelial cells upregulated genes involved in interferon signalling and antigen presentation during late infection, S. aureus downregulated toxin expression while upregulating genes related to iron scavenging. In conclusion, the presented data provide an important resource to facilitate functional investigations into host–pathogen interaction during S. aureus invasive infection and a basis for identifying novel drug target sites.Rasmus Birkholm GrønnemoseChristian GardeClaes Søndergaard WassmannJanne Kudsk KlitgaardRonni NielsenSusanne MandrupAndreas Holm MattssonThomas Emil AndersenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rasmus Birkholm Grønnemose Christian Garde Claes Søndergaard Wassmann Janne Kudsk Klitgaard Ronni Nielsen Susanne Mandrup Andreas Holm Mattsson Thomas Emil Andersen Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus
description	Abstract Staphylococcus aureus is the cause of serious vascular infections such as sepsis and endocarditis. These infections are notoriously difficult to treat, and it is believed that the ability of S. aureus to invade endothelial cells and persist intracellularly is a key mechanism for persistence despite ongoing antibiotic treatment. Here, we used dual RNA sequencing to study the simultaneous transcriptional response of S. aureus and human endothelial cells during in vitro infections. We revealed discrete and shared differentially expressed genes for both host and pathogen at the different stages of infection. While the endothelial cells upregulated genes involved in interferon signalling and antigen presentation during late infection, S. aureus downregulated toxin expression while upregulating genes related to iron scavenging. In conclusion, the presented data provide an important resource to facilitate functional investigations into host–pathogen interaction during S. aureus invasive infection and a basis for identifying novel drug target sites.
format	article
author	Rasmus Birkholm Grønnemose Christian Garde Claes Søndergaard Wassmann Janne Kudsk Klitgaard Ronni Nielsen Susanne Mandrup Andreas Holm Mattsson Thomas Emil Andersen
author_facet	Rasmus Birkholm Grønnemose Christian Garde Claes Søndergaard Wassmann Janne Kudsk Klitgaard Ronni Nielsen Susanne Mandrup Andreas Holm Mattsson Thomas Emil Andersen
author_sort	Rasmus Birkholm Grønnemose
title	Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus
title_short	Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus
title_full	Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus
title_fullStr	Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus
title_full_unstemmed	Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus
title_sort	bacteria-host transcriptional response during endothelial invasion by staphylococcus aureus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ebcbb48b41e24b86a95acf5c2d1df2bc
work_keys_str_mv	AT rasmusbirkholmgrønnemose bacteriahosttranscriptionalresponseduringendothelialinvasionbystaphylococcusaureus AT christiangarde bacteriahosttranscriptionalresponseduringendothelialinvasionbystaphylococcusaureus AT claessøndergaardwassmann bacteriahosttranscriptionalresponseduringendothelialinvasionbystaphylococcusaureus AT jannekudskklitgaard bacteriahosttranscriptionalresponseduringendothelialinvasionbystaphylococcusaureus AT ronninielsen bacteriahosttranscriptionalresponseduringendothelialinvasionbystaphylococcusaureus AT susannemandrup bacteriahosttranscriptionalresponseduringendothelialinvasionbystaphylococcusaureus AT andreasholmmattsson bacteriahosttranscriptionalresponseduringendothelialinvasionbystaphylococcusaureus AT thomasemilandersen bacteriahosttranscriptionalresponseduringendothelialinvasionbystaphylococcusaureus
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Bacteria-host transcriptional response during endothelial invasion by Staphylococcus aureus

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