Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles

Qingqing Dai,1,* Yasumasa Morita,2,* Yongbo Huang,3 Patricia C Liaw,4 Jianfeng Wu,5 Julie Khang,6 Diana Islam,6 Kaijiang Yu,7 Yimin Li,3 Haibo Zhang3,8–10 1Department of Critical Care Medicine, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, People&rsquo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dai Q, Morita Y, Huang Y, Liaw PC, Wu J, Khang J, Islam D, Yu K, Li Y, Zhang H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	defensins sepsis lung injury cytokines Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ebcc2ae0624a410994e9480442715c88
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ebcc2ae0624a410994e9480442715c88
record_format	dspace
spelling	oai:doaj.org-article:ebcc2ae0624a410994e9480442715c882021-12-02T05:04:50ZModulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles1178-7031https://doaj.org/article/ebcc2ae0624a410994e9480442715c882019-12-01T00:00:00Zhttps://www.dovepress.com/modulation-of-human-neutrophil-peptides-on-p-aeruginosa-killing-epithe-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Qingqing Dai,1,* Yasumasa Morita,2,* Yongbo Huang,3 Patricia C Liaw,4 Jianfeng Wu,5 Julie Khang,6 Diana Islam,6 Kaijiang Yu,7 Yimin Li,3 Haibo Zhang3,8–10 1Department of Critical Care Medicine, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, People’s Republic of China; 2Department of Emergency and Critical Care Medicine, Chiba Aoba Municipal Hospital, Chiba, Japan; 3The State Key Laboratory of Respiratory Disease, and The 1st Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of China; 4Department of Medicine, McMaster University, Hamilton, Canada; 5Department of Critical Care Medicine, The 1st Affiliated Hospital of Sun Yat-Sen University, Guangzhou, People’s Republic of China; 6Keenan Research Center for Biomedical Science of Unity Health Toronto, Toronto, Canada; 7Department of Critical Care Medicine, The 1st Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, People’s Republic of China; 8Interdepartmental Division of Critical Care Medicine, University of Toronto, Ontario, Canada; 9Departments of Anesthesia, University of Toronto, Ontario, Canada; 10Physiology, University of Toronto, Ontario, Canada*These authors contributed equally to this workCorrespondence: Kaijiang Yu 23 Youzheng Street, Harbin, Heilongjiang 150001, People’s Republic of ChinaTel/Fax +86 451-8555-2666Email drkaijiang@163.comYimin Li 151 Yanjiang Road West, Guangzhou, Guangdong 510120, People’s Republic of ChinaTel/Fax +86 20-8306-2961Email dryiminli@vip.163.comObjective: Neutrophil infiltration and release of the abundant human neutrophil peptides (HNP) are a common clinical feature in critically ill patients. We tested a hypothesis that different cell types respond to HNP differently in lung microenvironment that may influence the host responses.Methods: Plasma concentrations of HNP were measured in healthy volunteers and patients with sepsis. Cells including the bacteria P. aeruginosa, human lung epithelial cells and mesenchymal stromal cells (MSCs) were exposed to various concentrations of HNP. Bacterial killing, epithelial cell inflammation, MSC adhesion and behaviours were examined after HNP stimulation.Results: Incubation of P. aeruginosa or stimulation of human lung epithelial cells with HNP resulted in bacterial killing or IL-8 production at a dose of 50 μg/mL, while MSC adhesion and alternations of secretome profiles took place after HNP stimulation at a dose of 10 μg/mL. The secretome profile changes were characterized by increased release of the IL-6 family members such as C-reactive protein (CRP), leukemia inhibitory factor (LIF) and interleukin (IL-11), and first apoptosis signal (FAS) and platelet-derived growth factor-AA as compared to a vehicle control group.Conclusion: Stimulation of MSCs with HNP resulted in changes of secretome profiles at 5-fold lower concentration than that required for bacterial killing and lung epithelial inflammation. This undisclosed risk factor of HNP in lung environment should be taken into consideration when MSCs are applied as cell therapy in inflammatory lung diseases.Keywords: defensins, sepsis, lung injury, cytokinesDai QMorita YHuang YLiaw PCWu JKhang JIslam DYu KLi YZhang HDove Medical Pressarticledefensinssepsislung injurycytokinesPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 12, Pp 335-343 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	defensins sepsis lung injury cytokines Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	defensins sepsis lung injury cytokines Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Dai Q Morita Y Huang Y Liaw PC Wu J Khang J Islam D Yu K Li Y Zhang H Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles
description	Qingqing Dai,1,* Yasumasa Morita,2,* Yongbo Huang,3 Patricia C Liaw,4 Jianfeng Wu,5 Julie Khang,6 Diana Islam,6 Kaijiang Yu,7 Yimin Li,3 Haibo Zhang3,8–10 1Department of Critical Care Medicine, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, People’s Republic of China; 2Department of Emergency and Critical Care Medicine, Chiba Aoba Municipal Hospital, Chiba, Japan; 3The State Key Laboratory of Respiratory Disease, and The 1st Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of China; 4Department of Medicine, McMaster University, Hamilton, Canada; 5Department of Critical Care Medicine, The 1st Affiliated Hospital of Sun Yat-Sen University, Guangzhou, People’s Republic of China; 6Keenan Research Center for Biomedical Science of Unity Health Toronto, Toronto, Canada; 7Department of Critical Care Medicine, The 1st Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, People’s Republic of China; 8Interdepartmental Division of Critical Care Medicine, University of Toronto, Ontario, Canada; 9Departments of Anesthesia, University of Toronto, Ontario, Canada; 10Physiology, University of Toronto, Ontario, Canada*These authors contributed equally to this workCorrespondence: Kaijiang Yu 23 Youzheng Street, Harbin, Heilongjiang 150001, People’s Republic of ChinaTel/Fax +86 451-8555-2666Email drkaijiang@163.comYimin Li 151 Yanjiang Road West, Guangzhou, Guangdong 510120, People’s Republic of ChinaTel/Fax +86 20-8306-2961Email dryiminli@vip.163.comObjective: Neutrophil infiltration and release of the abundant human neutrophil peptides (HNP) are a common clinical feature in critically ill patients. We tested a hypothesis that different cell types respond to HNP differently in lung microenvironment that may influence the host responses.Methods: Plasma concentrations of HNP were measured in healthy volunteers and patients with sepsis. Cells including the bacteria P. aeruginosa, human lung epithelial cells and mesenchymal stromal cells (MSCs) were exposed to various concentrations of HNP. Bacterial killing, epithelial cell inflammation, MSC adhesion and behaviours were examined after HNP stimulation.Results: Incubation of P. aeruginosa or stimulation of human lung epithelial cells with HNP resulted in bacterial killing or IL-8 production at a dose of 50 μg/mL, while MSC adhesion and alternations of secretome profiles took place after HNP stimulation at a dose of 10 μg/mL. The secretome profile changes were characterized by increased release of the IL-6 family members such as C-reactive protein (CRP), leukemia inhibitory factor (LIF) and interleukin (IL-11), and first apoptosis signal (FAS) and platelet-derived growth factor-AA as compared to a vehicle control group.Conclusion: Stimulation of MSCs with HNP resulted in changes of secretome profiles at 5-fold lower concentration than that required for bacterial killing and lung epithelial inflammation. This undisclosed risk factor of HNP in lung environment should be taken into consideration when MSCs are applied as cell therapy in inflammatory lung diseases.Keywords: defensins, sepsis, lung injury, cytokines
format	article
author	Dai Q Morita Y Huang Y Liaw PC Wu J Khang J Islam D Yu K Li Y Zhang H
author_facet	Dai Q Morita Y Huang Y Liaw PC Wu J Khang J Islam D Yu K Li Y Zhang H
author_sort	Dai Q
title	Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles
title_short	Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles
title_full	Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles
title_fullStr	Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles
title_full_unstemmed	Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles
title_sort	modulation of human neutrophil peptides on p. aeruginosa killing, epithelial cell inflammation and mesenchymal stromal cell secretome profiles
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/ebcc2ae0624a410994e9480442715c88
work_keys_str_mv	AT daiq modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles AT moritay modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles AT huangy modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles AT liawpc modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles AT wuj modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles AT khangj modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles AT islamd modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles AT yuk modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles AT liy modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles AT zhangh modulationofhumanneutrophilpeptidesonpaeruginosakillingepithelialcellinflammationandmesenchymalstromalcellsecretomeprofiles
_version_	1718400625938530304

Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles

Ejemplares similares