Evaluation of methods for the reduction of contaminating host reads when performing shotgun metagenomic sequencing of the milk microbiome

Abstract Shotgun metagenomic sequencing is a valuable tool for the taxonomic and functional profiling of microbial communities. However, this approach is challenging in samples, such as milk, where a low microbial abundance, combined with high levels of host DNA, result in inefficient and uneconomic...

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Main Authors: Min Yap, Conor Feehily, Calum J. Walsh, Mark Fenelon, Eileen F. Murphy, Fionnuala M. McAuliffe, Douwe van Sinderen, Paul W. O’Toole, Orla O’Sullivan, Paul D. Cotter
Format: article
Language:EN
Published: Nature Portfolio 2020
Subjects:
R
Q
Online Access:https://doaj.org/article/ebd35d15f2fe424d9aae078bc49b7de4
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