High-resolution label-free 3D mapping of extracellular pH of single living cells

Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D ma...

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Autores principales:	Yanjun Zhang, Yasufumi Takahashi, Sung Pil Hong, Fengjie Liu, Joanna Bednarska, Philip S. Goff, Pavel Novak, Andrew Shevchuk, Sahana Gopal, Iros Barozzi, Luca Magnani, Hideki Sakai, Yoshimoto Suguru, Takuto Fujii, Alexander Erofeev, Peter Gorelkin, Alexander Majouga, Dominik J. Weiss, Christopher Edwards, Aleksandar P. Ivanov, David Klenerman, Elena V. Sviderskaya, Joshua B. Edel, Yuri Korchev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ebd98ca7f57c46df9f47297895059247
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spelling	oai:doaj.org-article:ebd98ca7f57c46df9f472978950592472021-12-02T14:35:55ZHigh-resolution label-free 3D mapping of extracellular pH of single living cells10.1038/s41467-019-13535-12041-1723https://doaj.org/article/ebd98ca7f57c46df9f472978950592472019-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13535-1https://doaj.org/toc/2041-1723Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D mapping in live cancer cells.Yanjun ZhangYasufumi TakahashiSung Pil HongFengjie LiuJoanna BednarskaPhilip S. GoffPavel NovakAndrew ShevchukSahana GopalIros BarozziLuca MagnaniHideki SakaiYoshimoto SuguruTakuto FujiiAlexander ErofeevPeter GorelkinAlexander MajougaDominik J. WeissChristopher EdwardsAleksandar P. IvanovDavid KlenermanElena V. SviderskayaJoshua B. EdelYuri KorchevNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yanjun Zhang Yasufumi Takahashi Sung Pil Hong Fengjie Liu Joanna Bednarska Philip S. Goff Pavel Novak Andrew Shevchuk Sahana Gopal Iros Barozzi Luca Magnani Hideki Sakai Yoshimoto Suguru Takuto Fujii Alexander Erofeev Peter Gorelkin Alexander Majouga Dominik J. Weiss Christopher Edwards Aleksandar P. Ivanov David Klenerman Elena V. Sviderskaya Joshua B. Edel Yuri Korchev High-resolution label-free 3D mapping of extracellular pH of single living cells
description	Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D mapping in live cancer cells.
format	article
author	Yanjun Zhang Yasufumi Takahashi Sung Pil Hong Fengjie Liu Joanna Bednarska Philip S. Goff Pavel Novak Andrew Shevchuk Sahana Gopal Iros Barozzi Luca Magnani Hideki Sakai Yoshimoto Suguru Takuto Fujii Alexander Erofeev Peter Gorelkin Alexander Majouga Dominik J. Weiss Christopher Edwards Aleksandar P. Ivanov David Klenerman Elena V. Sviderskaya Joshua B. Edel Yuri Korchev
author_facet	Yanjun Zhang Yasufumi Takahashi Sung Pil Hong Fengjie Liu Joanna Bednarska Philip S. Goff Pavel Novak Andrew Shevchuk Sahana Gopal Iros Barozzi Luca Magnani Hideki Sakai Yoshimoto Suguru Takuto Fujii Alexander Erofeev Peter Gorelkin Alexander Majouga Dominik J. Weiss Christopher Edwards Aleksandar P. Ivanov David Klenerman Elena V. Sviderskaya Joshua B. Edel Yuri Korchev
author_sort	Yanjun Zhang
title	High-resolution label-free 3D mapping of extracellular pH of single living cells
title_short	High-resolution label-free 3D mapping of extracellular pH of single living cells
title_full	High-resolution label-free 3D mapping of extracellular pH of single living cells
title_fullStr	High-resolution label-free 3D mapping of extracellular pH of single living cells
title_full_unstemmed	High-resolution label-free 3D mapping of extracellular pH of single living cells
title_sort	high-resolution label-free 3d mapping of extracellular ph of single living cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ebd98ca7f57c46df9f47297895059247
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High-resolution label-free 3D mapping of extracellular pH of single living cells

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