Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway

The study aimed to investigate the effect of erdosteine on middle ear effusion in rats through mediating the Toll-like receptor 4 (TLR4) signaling pathway. Rats were injected with endotoxin to prepare the model of acute secretory otitis media (SOM). Then, they were divided into an acute SOM model gr...

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Autores principales:	Te Li, Wanting Zeng, Rongrong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/ebe035fe23294f6897e9321ffc263ec8
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spelling	oai:doaj.org-article:ebe035fe23294f6897e9321ffc263ec82021-11-08T02:35:15ZEffect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway2314-614110.1155/2021/9968907https://doaj.org/article/ebe035fe23294f6897e9321ffc263ec82021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9968907https://doaj.org/toc/2314-6141The study aimed to investigate the effect of erdosteine on middle ear effusion in rats through mediating the Toll-like receptor 4 (TLR4) signaling pathway. Rats were injected with endotoxin to prepare the model of acute secretory otitis media (SOM). Then, they were divided into an acute SOM model group (model group, n=15) and erdosteine treatment group (18 mg/kg, gavage, treatment group, n=15). Besides, a normal group (n=15) was set up. Two weeks later, routine biochemical indicators such as aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were detected. The inflammatory effusion due to otitis media was scored. The content of myeloperoxidase (MPO), matrix metalloproteinase (MMP), and tumor necrosis factor-beta (TNF-β) in middle ear lavage fluid was detected via enzyme-linked immunosorbent assay (ELISA). Additionally, histomorphological changes were observed with the help of hematoxylin-eosin (HE) staining, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting assays were carried out to measure the expression levels of TLR4 pathway genes and proteins as well as the messenger ribonucleic acid (mRNA) expression levels of key factors for otitis media (mucin 2 (MUC2) and MUC5A). In the model group, the levels of AST, ALP, and glutamic-pyruvic transaminase (GPT) were significantly increased (p<0.05). Besides, the content of MPO, MMP, and TNF-β was overtly raised in the model group (p<0.05), while it was notably lowered in the treatment group (p<0.05). In the treatment group, the cilia were slightly swollen, and inflammatory cells were fewer. The mRNA levels of MUC2, MUC5A, and pathway genes TLR4 and c-Jun N-terminal kinase (JNK) were elevated in the model group. In addition, the protein assay results revealed that the protein levels of TLR4 and JNK were evidently increased in the model group. Erdosteine can treat the middle ear effusion in rats by repressing the activation of the TLR4 signaling pathway.Te LiWanting ZengRongrong LiuHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Te Li Wanting Zeng Rongrong Liu Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway
description	The study aimed to investigate the effect of erdosteine on middle ear effusion in rats through mediating the Toll-like receptor 4 (TLR4) signaling pathway. Rats were injected with endotoxin to prepare the model of acute secretory otitis media (SOM). Then, they were divided into an acute SOM model group (model group, n=15) and erdosteine treatment group (18 mg/kg, gavage, treatment group, n=15). Besides, a normal group (n=15) was set up. Two weeks later, routine biochemical indicators such as aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were detected. The inflammatory effusion due to otitis media was scored. The content of myeloperoxidase (MPO), matrix metalloproteinase (MMP), and tumor necrosis factor-beta (TNF-β) in middle ear lavage fluid was detected via enzyme-linked immunosorbent assay (ELISA). Additionally, histomorphological changes were observed with the help of hematoxylin-eosin (HE) staining, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting assays were carried out to measure the expression levels of TLR4 pathway genes and proteins as well as the messenger ribonucleic acid (mRNA) expression levels of key factors for otitis media (mucin 2 (MUC2) and MUC5A). In the model group, the levels of AST, ALP, and glutamic-pyruvic transaminase (GPT) were significantly increased (p<0.05). Besides, the content of MPO, MMP, and TNF-β was overtly raised in the model group (p<0.05), while it was notably lowered in the treatment group (p<0.05). In the treatment group, the cilia were slightly swollen, and inflammatory cells were fewer. The mRNA levels of MUC2, MUC5A, and pathway genes TLR4 and c-Jun N-terminal kinase (JNK) were elevated in the model group. In addition, the protein assay results revealed that the protein levels of TLR4 and JNK were evidently increased in the model group. Erdosteine can treat the middle ear effusion in rats by repressing the activation of the TLR4 signaling pathway.
format	article
author	Te Li Wanting Zeng Rongrong Liu
author_facet	Te Li Wanting Zeng Rongrong Liu
author_sort	Te Li
title	Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway
title_short	Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway
title_full	Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway
title_fullStr	Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway
title_full_unstemmed	Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway
title_sort	effect of erdosteine on middle ear effusion in rats by mediating tlr4 signaling pathway
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/ebe035fe23294f6897e9321ffc263ec8
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Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway

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