Structural insights into DNA cleavage activation of CRISPR-Cas9 system

CRISPR-Cas9 is widely used for genome engineering but structural data for the DNA cleavage step are still incomplete. Here, the authors present the cryo-EM structure of a ternary Cas9-sgRNA-target DNA complex, perform MD simulations and discuss implications for the Cas9 DNA cleavage mechanism.

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Autores principales:	Cong Huai, Gan Li, Ruijie Yao, Yingyi Zhang, Mi Cao, Liangliang Kong, Chenqiang Jia, Hui Yuan, Hongyan Chen, Daru Lu, Qiang Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ebe04912ba9f4c5289a0cf88e17df27a
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spelling	oai:doaj.org-article:ebe04912ba9f4c5289a0cf88e17df27a2021-12-02T17:01:17ZStructural insights into DNA cleavage activation of CRISPR-Cas9 system10.1038/s41467-017-01496-22041-1723https://doaj.org/article/ebe04912ba9f4c5289a0cf88e17df27a2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01496-2https://doaj.org/toc/2041-1723CRISPR-Cas9 is widely used for genome engineering but structural data for the DNA cleavage step are still incomplete. Here, the authors present the cryo-EM structure of a ternary Cas9-sgRNA-target DNA complex, perform MD simulations and discuss implications for the Cas9 DNA cleavage mechanism.Cong HuaiGan LiRuijie YaoYingyi ZhangMi CaoLiangliang KongChenqiang JiaHui YuanHongyan ChenDaru LuQiang HuangNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Cong Huai Gan Li Ruijie Yao Yingyi Zhang Mi Cao Liangliang Kong Chenqiang Jia Hui Yuan Hongyan Chen Daru Lu Qiang Huang Structural insights into DNA cleavage activation of CRISPR-Cas9 system
description	CRISPR-Cas9 is widely used for genome engineering but structural data for the DNA cleavage step are still incomplete. Here, the authors present the cryo-EM structure of a ternary Cas9-sgRNA-target DNA complex, perform MD simulations and discuss implications for the Cas9 DNA cleavage mechanism.
format	article
author	Cong Huai Gan Li Ruijie Yao Yingyi Zhang Mi Cao Liangliang Kong Chenqiang Jia Hui Yuan Hongyan Chen Daru Lu Qiang Huang
author_facet	Cong Huai Gan Li Ruijie Yao Yingyi Zhang Mi Cao Liangliang Kong Chenqiang Jia Hui Yuan Hongyan Chen Daru Lu Qiang Huang
author_sort	Cong Huai
title	Structural insights into DNA cleavage activation of CRISPR-Cas9 system
title_short	Structural insights into DNA cleavage activation of CRISPR-Cas9 system
title_full	Structural insights into DNA cleavage activation of CRISPR-Cas9 system
title_fullStr	Structural insights into DNA cleavage activation of CRISPR-Cas9 system
title_full_unstemmed	Structural insights into DNA cleavage activation of CRISPR-Cas9 system
title_sort	structural insights into dna cleavage activation of crispr-cas9 system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ebe04912ba9f4c5289a0cf88e17df27a
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Structural insights into DNA cleavage activation of CRISPR-Cas9 system

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