Monte Carlo Simulation of Double-Strand Break Induction and Conversion after Ultrasoft X-rays Irradiation

This paper estimates the yields of DNA double-strand breaks (DSBs) induced by ultrasoft X-rays and uses the DSB yields and the repair outcomes to evaluate the relative biological effectiveness (<i>RBE</i>) of ultrasoft X-rays. We simulated the yields of DSB induction and predicted them i...

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Autores principales:	Ya-Yun Hsiao, Fang-Hsin Chen, Chun-Chieh Chan, Ching-Chih Tsai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	DSB induction ultrasoft X-rays DNA repair enzymatic DSB Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/ebe253ae6c4541db83ccae76932f763c
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spelling	oai:doaj.org-article:ebe253ae6c4541db83ccae76932f763c2021-11-11T17:10:10ZMonte Carlo Simulation of Double-Strand Break Induction and Conversion after Ultrasoft X-rays Irradiation10.3390/ijms2221117131422-00671661-6596https://doaj.org/article/ebe253ae6c4541db83ccae76932f763c2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11713https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067This paper estimates the yields of DNA double-strand breaks (DSBs) induced by ultrasoft X-rays and uses the DSB yields and the repair outcomes to evaluate the relative biological effectiveness (<i>RBE</i>) of ultrasoft X-rays. We simulated the yields of DSB induction and predicted them in the presence and absence of oxygen, using a Monte Carlo damage simulation (MCDS) software, to calculate the <i>RBE</i>. Monte Carlo excision repair (MCER) simulations were also performed to calculate the repair outcomes (correct repairs, mutations, and DSB conversions). Compared to <sup>60</sup>Co γ-rays, the <i>RBE</i> values for ultrasoft X-rays (titanium K-shell, aluminum K-shell, copper L-shell, and carbon K-shell) for DSB induction were respectively 1.3, 1.9, 2.3, and 2.6 under aerobic conditions and 1.3, 2.1, 2.5, and 2.9 under a hypoxic condition (2% O<sub>2</sub>). The <i>RBE</i> values for enzymatic DSBs were 1.6, 2.1, 2.3, and 2.4, respectively, indicating that the enzymatic DSB yields are comparable to the yields of DSB induction. The synergistic effects of DSB induction and enzymatic DSB formation further facilitate cell killing and the advantage in cancer treatment.Ya-Yun HsiaoFang-Hsin ChenChun-Chieh ChanChing-Chih TsaiMDPI AGarticleDSB inductionultrasoft X-raysDNA repairenzymatic DSBBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11713, p 11713 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	DSB induction ultrasoft X-rays DNA repair enzymatic DSB Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	DSB induction ultrasoft X-rays DNA repair enzymatic DSB Biology (General) QH301-705.5 Chemistry QD1-999 Ya-Yun Hsiao Fang-Hsin Chen Chun-Chieh Chan Ching-Chih Tsai Monte Carlo Simulation of Double-Strand Break Induction and Conversion after Ultrasoft X-rays Irradiation
description	This paper estimates the yields of DNA double-strand breaks (DSBs) induced by ultrasoft X-rays and uses the DSB yields and the repair outcomes to evaluate the relative biological effectiveness (<i>RBE</i>) of ultrasoft X-rays. We simulated the yields of DSB induction and predicted them in the presence and absence of oxygen, using a Monte Carlo damage simulation (MCDS) software, to calculate the <i>RBE</i>. Monte Carlo excision repair (MCER) simulations were also performed to calculate the repair outcomes (correct repairs, mutations, and DSB conversions). Compared to <sup>60</sup>Co γ-rays, the <i>RBE</i> values for ultrasoft X-rays (titanium K-shell, aluminum K-shell, copper L-shell, and carbon K-shell) for DSB induction were respectively 1.3, 1.9, 2.3, and 2.6 under aerobic conditions and 1.3, 2.1, 2.5, and 2.9 under a hypoxic condition (2% O<sub>2</sub>). The <i>RBE</i> values for enzymatic DSBs were 1.6, 2.1, 2.3, and 2.4, respectively, indicating that the enzymatic DSB yields are comparable to the yields of DSB induction. The synergistic effects of DSB induction and enzymatic DSB formation further facilitate cell killing and the advantage in cancer treatment.
format	article
author	Ya-Yun Hsiao Fang-Hsin Chen Chun-Chieh Chan Ching-Chih Tsai
author_facet	Ya-Yun Hsiao Fang-Hsin Chen Chun-Chieh Chan Ching-Chih Tsai
author_sort	Ya-Yun Hsiao
title	Monte Carlo Simulation of Double-Strand Break Induction and Conversion after Ultrasoft X-rays Irradiation
title_short	Monte Carlo Simulation of Double-Strand Break Induction and Conversion after Ultrasoft X-rays Irradiation
title_full	Monte Carlo Simulation of Double-Strand Break Induction and Conversion after Ultrasoft X-rays Irradiation
title_fullStr	Monte Carlo Simulation of Double-Strand Break Induction and Conversion after Ultrasoft X-rays Irradiation
title_full_unstemmed	Monte Carlo Simulation of Double-Strand Break Induction and Conversion after Ultrasoft X-rays Irradiation
title_sort	monte carlo simulation of double-strand break induction and conversion after ultrasoft x-rays irradiation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ebe253ae6c4541db83ccae76932f763c
work_keys_str_mv	AT yayunhsiao montecarlosimulationofdoublestrandbreakinductionandconversionafterultrasoftxraysirradiation AT fanghsinchen montecarlosimulationofdoublestrandbreakinductionandconversionafterultrasoftxraysirradiation AT chunchiehchan montecarlosimulationofdoublestrandbreakinductionandconversionafterultrasoftxraysirradiation AT chingchihtsai montecarlosimulationofdoublestrandbreakinductionandconversionafterultrasoftxraysirradiation
_version_	1718432210837569536

Monte Carlo Simulation of Double-Strand Break Induction and Conversion after Ultrasoft X-rays Irradiation

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