Antibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans

Abstract Objective Dental caries is a high prevalent chronic bacterial infectious disease caused by plaque, a bacterial colony deposited on tooth surfaces and gum tissues. Streptococcus mutans is a primary cariogenic bacterium commonly found in the human oral cavity. Oral hygiene products containing...

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Autores principales:	Yea Ji Nam, Young Sun Hwang
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	dental caries ethanol extract of Terminalia chebula plaque Streptococcus mutans Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/ebe572b79fd7418baf197b0726a88349
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spelling	oai:doaj.org-article:ebe572b79fd7418baf197b0726a883492021-12-02T12:59:21ZAntibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans2057-434710.1002/cre2.467https://doaj.org/article/ebe572b79fd7418baf197b0726a883492021-12-01T00:00:00Zhttps://doi.org/10.1002/cre2.467https://doaj.org/toc/2057-4347Abstract Objective Dental caries is a high prevalent chronic bacterial infectious disease caused by plaque, a bacterial colony deposited on tooth surfaces and gum tissues. Streptococcus mutans is a primary cariogenic bacterium commonly found in the human oral cavity. Oral hygiene products containing antibacterial ingredients can be helpful in caries management. In this study, we investigated the anticaries mechanism of the ethanol extract of Terminalia chebula (EETC) on S. mutans and suggest its possible application as a functional ingredients for oral hygiene products. Materials and methods The EETC was prepared from the Terminalia chebula fruit. Disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and colony forming unit (CFU) were analyzed to observe the antibacterial activity of EETC. The glucan formation was measured using the filtrate of bacterial culture medium and sucrose. Gene expression was analyzed using RT‐PCR. Cytotoxicity was analyzed using the MTT assay. The radical‐scavenging activities of DPPH and ABTS were also tested to verify the antioxidant activity of EETC. Results The antibacterial activity of the EETC was explored through a disc diffusion analysis and CFU measurement. EETC treatment decreased insoluble glucan formation and gene expression of glycosyltransferase B (gtf B), glycosyltransferase C (gtf C), glycosyltransferase D (gtf D), and fructosyltransferase (ftf). The MIC and MBC of EETC on S. mutans were not cytotoxic to gingival fibroblasts. In addition, we observed DPPH and ABTS‐radical scavenging activities of EETC. Conclusions These results indicate that the antibacterial and antioxidant effects of EETC may contribute to oral hygiene products for dental caries management.Yea Ji NamYoung Sun HwangWileyarticledental cariesethanol extract of Terminalia chebulaplaqueStreptococcus mutansDentistryRK1-715ENClinical and Experimental Dental Research, Vol 7, Iss 6, Pp 987-994 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dental caries ethanol extract of Terminalia chebula plaque Streptococcus mutans Dentistry RK1-715
spellingShingle	dental caries ethanol extract of Terminalia chebula plaque Streptococcus mutans Dentistry RK1-715 Yea Ji Nam Young Sun Hwang Antibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans
description	Abstract Objective Dental caries is a high prevalent chronic bacterial infectious disease caused by plaque, a bacterial colony deposited on tooth surfaces and gum tissues. Streptococcus mutans is a primary cariogenic bacterium commonly found in the human oral cavity. Oral hygiene products containing antibacterial ingredients can be helpful in caries management. In this study, we investigated the anticaries mechanism of the ethanol extract of Terminalia chebula (EETC) on S. mutans and suggest its possible application as a functional ingredients for oral hygiene products. Materials and methods The EETC was prepared from the Terminalia chebula fruit. Disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and colony forming unit (CFU) were analyzed to observe the antibacterial activity of EETC. The glucan formation was measured using the filtrate of bacterial culture medium and sucrose. Gene expression was analyzed using RT‐PCR. Cytotoxicity was analyzed using the MTT assay. The radical‐scavenging activities of DPPH and ABTS were also tested to verify the antioxidant activity of EETC. Results The antibacterial activity of the EETC was explored through a disc diffusion analysis and CFU measurement. EETC treatment decreased insoluble glucan formation and gene expression of glycosyltransferase B (gtf B), glycosyltransferase C (gtf C), glycosyltransferase D (gtf D), and fructosyltransferase (ftf). The MIC and MBC of EETC on S. mutans were not cytotoxic to gingival fibroblasts. In addition, we observed DPPH and ABTS‐radical scavenging activities of EETC. Conclusions These results indicate that the antibacterial and antioxidant effects of EETC may contribute to oral hygiene products for dental caries management.
format	article
author	Yea Ji Nam Young Sun Hwang
author_facet	Yea Ji Nam Young Sun Hwang
author_sort	Yea Ji Nam
title	Antibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans
title_short	Antibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans
title_full	Antibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans
title_fullStr	Antibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans
title_full_unstemmed	Antibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans
title_sort	antibacterial and antioxidant effect of ethanol extracts of terminalia chebula on streptococcus mutans
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/ebe572b79fd7418baf197b0726a88349
work_keys_str_mv	AT yeajinam antibacterialandantioxidanteffectofethanolextractsofterminaliachebulaonstreptococcusmutans AT youngsunhwang antibacterialandantioxidanteffectofethanolextractsofterminaliachebulaonstreptococcusmutans
_version_	1718393599687655424

Antibacterial and antioxidant effect of ethanol extracts of Terminalia chebula on Streptococcus mutans

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