In vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects

Jyoti V Kumbhar,1 Sachin H Jadhav,2 Dhananjay S Bodas,1 Amruta Barhanpurkar-Naik,3 Mohan R Wani,3 Kishore M Paknikar,1 Jyutika M Rajwade1 1Nanobioscience, 2Animal Sciences Division, Agharkar Research Institute, 3National Centre for Cell Science, Savitribai Phule Pune University, Pune, India Abstra...

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Autores principales:	Kumbhar JV, Jadhav SH, Bodas DS, Barhanpurkar-Naik A, Wani MR, Paknikar KM, Rajwade JM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Bacterial cellulose-hydroxyapatite Bacterial cellulose-glycosaminoglycan osteochondral defect stem cell differentiation acellular bilayer scaffold Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ebe660547027403f8f6bcbf81d8ea386
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spelling	oai:doaj.org-article:ebe660547027403f8f6bcbf81d8ea3862021-12-02T00:07:18ZIn vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects1178-2013https://doaj.org/article/ebe660547027403f8f6bcbf81d8ea3862017-09-01T00:00:00Zhttps://www.dovepress.com/in-vitro-and-in-vivo-studies-of-a-novel-bacterial-cellulose-based-acel-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jyoti V Kumbhar,1 Sachin H Jadhav,2 Dhananjay S Bodas,1 Amruta Barhanpurkar-Naik,3 Mohan R Wani,3 Kishore M Paknikar,1 Jyutika M Rajwade1 1Nanobioscience, 2Animal Sciences Division, Agharkar Research Institute, 3National Centre for Cell Science, Savitribai Phule Pune University, Pune, India Abstract: Bacterial cellulose (BC) is a naturally occurring nanofibrous biomaterial which exhibits unique physical properties and is amenable to chemical modifications. To explore whether this versatile material can be used in the treatment of osteochondral defects (OCD), we developed and characterized novel BC-based nanocomposite scaffolds, for example, BC-hydroxyapatite (BC-HA) and BC-glycosaminoglycans (BC-GAG) that mimic bone and cartilage, respectively. In vitro biocompatibility of BC-HA and BC-GAG scaffolds was established using osteosarcoma cells, human articular chondrocytes, and human adipose-derived mesenchymal stem cells. On subcutaneous implantation, the scaffolds allowed tissue ingrowth and induced no adverse immunological reactions suggesting excellent in vivo biocompatibility. Implantation of acellular bilayered scaffolds in OCD created in rat knees induced progressive regeneration of cartilage tissue, deposition of extracellular matrix, and regeneration of subchondral bone by the host cells. The results of micro-CT revealed that bone mineral density and ratio of bone volume to tissue volume were significantly higher in animals receiving bilayered scaffold as compared to the control animals. To the best of our knowledge, this study proves for the first time, the functional performance of acellular BC-based bilayered scaffolds. Thus, this strategy has great potential for clinical translation and can be used in repair of OCD. Keywords: bacterial cellulose-hydroxyapatite, bacterial cellulose-glycosaminoglycan, osteochondral defect, stem cell differentiation, acellular bilayer scaffold Kumbhar JVJadhav SHBodas DSBarhanpurkar-Naik AWani MRPaknikar KMRajwade JMDove Medical PressarticleBacterial cellulose-hydroxyapatiteBacterial cellulose-glycosaminoglycanosteochondral defectstem cell differentiationacellular bilayer scaffoldMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6437-6459 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacterial cellulose-hydroxyapatite Bacterial cellulose-glycosaminoglycan osteochondral defect stem cell differentiation acellular bilayer scaffold Medicine (General) R5-920
spellingShingle	Bacterial cellulose-hydroxyapatite Bacterial cellulose-glycosaminoglycan osteochondral defect stem cell differentiation acellular bilayer scaffold Medicine (General) R5-920 Kumbhar JV Jadhav SH Bodas DS Barhanpurkar-Naik A Wani MR Paknikar KM Rajwade JM In vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects
description	Jyoti V Kumbhar,1 Sachin H Jadhav,2 Dhananjay S Bodas,1 Amruta Barhanpurkar-Naik,3 Mohan R Wani,3 Kishore M Paknikar,1 Jyutika M Rajwade1 1Nanobioscience, 2Animal Sciences Division, Agharkar Research Institute, 3National Centre for Cell Science, Savitribai Phule Pune University, Pune, India Abstract: Bacterial cellulose (BC) is a naturally occurring nanofibrous biomaterial which exhibits unique physical properties and is amenable to chemical modifications. To explore whether this versatile material can be used in the treatment of osteochondral defects (OCD), we developed and characterized novel BC-based nanocomposite scaffolds, for example, BC-hydroxyapatite (BC-HA) and BC-glycosaminoglycans (BC-GAG) that mimic bone and cartilage, respectively. In vitro biocompatibility of BC-HA and BC-GAG scaffolds was established using osteosarcoma cells, human articular chondrocytes, and human adipose-derived mesenchymal stem cells. On subcutaneous implantation, the scaffolds allowed tissue ingrowth and induced no adverse immunological reactions suggesting excellent in vivo biocompatibility. Implantation of acellular bilayered scaffolds in OCD created in rat knees induced progressive regeneration of cartilage tissue, deposition of extracellular matrix, and regeneration of subchondral bone by the host cells. The results of micro-CT revealed that bone mineral density and ratio of bone volume to tissue volume were significantly higher in animals receiving bilayered scaffold as compared to the control animals. To the best of our knowledge, this study proves for the first time, the functional performance of acellular BC-based bilayered scaffolds. Thus, this strategy has great potential for clinical translation and can be used in repair of OCD. Keywords: bacterial cellulose-hydroxyapatite, bacterial cellulose-glycosaminoglycan, osteochondral defect, stem cell differentiation, acellular bilayer scaffold
format	article
author	Kumbhar JV Jadhav SH Bodas DS Barhanpurkar-Naik A Wani MR Paknikar KM Rajwade JM
author_facet	Kumbhar JV Jadhav SH Bodas DS Barhanpurkar-Naik A Wani MR Paknikar KM Rajwade JM
author_sort	Kumbhar JV
title	In vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects
title_short	In vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects
title_full	In vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects
title_fullStr	In vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects
title_full_unstemmed	In vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects
title_sort	in vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/ebe660547027403f8f6bcbf81d8ea386
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In vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects

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