MiRNA-200b reverses drug resistance of hepatocellular carcinoma HepG2 cells to sorafenib and its underlying mechanism

Objective To explore the effect of miRNA-200b on sorafenib resistance in hepatocellular carcinoma cells in order to increase the efficacy of the drug in advanced liver cancer patients. Methods Sorafenib resistant hepatocellular carcinoma HepG2-SR cell line was established by gradually increasing th...

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Autores principales:	DENG Yong, TANG Tengqian, ZHANG Leida
Formato:	article
Lenguaje:	ZH
Publicado:	Editorial Office of Journal of Third Military Medical University 2021
Materias:	hepatocellular carcinoma mirna-200b sorafenib drug resistance Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ebebf18ffa6548c1a4574feac02cd3e2
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spelling	oai:doaj.org-article:ebebf18ffa6548c1a4574feac02cd3e22021-11-28T10:47:47ZMiRNA-200b reverses drug resistance of hepatocellular carcinoma HepG2 cells to sorafenib and its underlying mechanism10.16016/j.1000-5404.2021050851000-5404https://doaj.org/article/ebebf18ffa6548c1a4574feac02cd3e22021-11-01T00:00:00Zhttp://aammt.tmmu.edu.cn/Upload/rhtml/202105085.htmhttps://doaj.org/toc/1000-5404 Objective To explore the effect of miRNA-200b on sorafenib resistance in hepatocellular carcinoma cells in order to increase the efficacy of the drug in advanced liver cancer patients. Methods Sorafenib resistant hepatocellular carcinoma HepG2-SR cell line was established by gradually increasing the concentration of sorafenib in the culture medium. The expression of miRNA-200b in HepG2-SR cells, HepG2 cells and miRNA-200b transfected HepG2-SR cells was detected by real-time fluorescence quantitative assay (RT-PCR). In HepG2-SR cells before and after transfection, sorafenib sensitivity was detected by CCK-8 assay, migration and invasion abilities were detected by wound-healing assay and Transwell assay, cell apoptosis was measured by flow cytometry, and expression levels of epithelial mesenchymal transition (EMT) markers were studied by Western blotting. Results Sorafenib resistance was significantly higher and expression of miRNA-200b was significantly lower in HepG2-SR cells than HepG2 cells. Transfection of miRNA-200b mimics resulted in significantly increased miRNA-200b expression, decreased IC50 of sorafenib, reduced cell migration rate and cell invasion, enhanced apoptosis and increased expression of E-cadherin but decreased expression of N-cadherin and Vimentin. Conclusion MiRNA-200b reversed sorafenib resistance of HepG2-SR cells by inducing apoptosis and inhibiting cell proliferation, migration, invasion and EMT.DENG YongTANG TengqianZHANG LeidaEditorial Office of Journal of Third Military Medical Universityarticlehepatocellular carcinomamirna-200bsorafenibdrug resistanceMedicine (General)R5-920ZHDi-san junyi daxue xuebao, Vol 43, Iss 22, Pp 2435-2440 (2021)
institution	DOAJ
collection	DOAJ
language	ZH
topic	hepatocellular carcinoma mirna-200b sorafenib drug resistance Medicine (General) R5-920
spellingShingle	hepatocellular carcinoma mirna-200b sorafenib drug resistance Medicine (General) R5-920 DENG Yong TANG Tengqian ZHANG Leida MiRNA-200b reverses drug resistance of hepatocellular carcinoma HepG2 cells to sorafenib and its underlying mechanism
description	Objective To explore the effect of miRNA-200b on sorafenib resistance in hepatocellular carcinoma cells in order to increase the efficacy of the drug in advanced liver cancer patients. Methods Sorafenib resistant hepatocellular carcinoma HepG2-SR cell line was established by gradually increasing the concentration of sorafenib in the culture medium. The expression of miRNA-200b in HepG2-SR cells, HepG2 cells and miRNA-200b transfected HepG2-SR cells was detected by real-time fluorescence quantitative assay (RT-PCR). In HepG2-SR cells before and after transfection, sorafenib sensitivity was detected by CCK-8 assay, migration and invasion abilities were detected by wound-healing assay and Transwell assay, cell apoptosis was measured by flow cytometry, and expression levels of epithelial mesenchymal transition (EMT) markers were studied by Western blotting. Results Sorafenib resistance was significantly higher and expression of miRNA-200b was significantly lower in HepG2-SR cells than HepG2 cells. Transfection of miRNA-200b mimics resulted in significantly increased miRNA-200b expression, decreased IC50 of sorafenib, reduced cell migration rate and cell invasion, enhanced apoptosis and increased expression of E-cadherin but decreased expression of N-cadherin and Vimentin. Conclusion MiRNA-200b reversed sorafenib resistance of HepG2-SR cells by inducing apoptosis and inhibiting cell proliferation, migration, invasion and EMT.
format	article
author	DENG Yong TANG Tengqian ZHANG Leida
author_facet	DENG Yong TANG Tengqian ZHANG Leida
author_sort	DENG Yong
title	MiRNA-200b reverses drug resistance of hepatocellular carcinoma HepG2 cells to sorafenib and its underlying mechanism
title_short	MiRNA-200b reverses drug resistance of hepatocellular carcinoma HepG2 cells to sorafenib and its underlying mechanism
title_full	MiRNA-200b reverses drug resistance of hepatocellular carcinoma HepG2 cells to sorafenib and its underlying mechanism
title_fullStr	MiRNA-200b reverses drug resistance of hepatocellular carcinoma HepG2 cells to sorafenib and its underlying mechanism
title_full_unstemmed	MiRNA-200b reverses drug resistance of hepatocellular carcinoma HepG2 cells to sorafenib and its underlying mechanism
title_sort	mirna-200b reverses drug resistance of hepatocellular carcinoma hepg2 cells to sorafenib and its underlying mechanism
publisher	Editorial Office of Journal of Third Military Medical University
publishDate	2021
url	https://doaj.org/article/ebebf18ffa6548c1a4574feac02cd3e2
work_keys_str_mv	AT dengyong mirna200breversesdrugresistanceofhepatocellularcarcinomahepg2cellstosorafenibanditsunderlyingmechanism AT tangtengqian mirna200breversesdrugresistanceofhepatocellularcarcinomahepg2cellstosorafenibanditsunderlyingmechanism AT zhangleida mirna200breversesdrugresistanceofhepatocellularcarcinomahepg2cellstosorafenibanditsunderlyingmechanism
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MiRNA-200b reverses drug resistance of hepatocellular carcinoma HepG2 cells to sorafenib and its underlying mechanism

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