SARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.

SARS-CoV-2 is the new coronavirus responsible for COVID-19 disease. The first two cases of COVID-19 were detected in Venezuela on March 13, 2020. The aim of this study was the genetic characterization of Venezuelan SARS-CoV-2 isolates. A total of 7 full SARS-CoV-2 genome sequences were obtained by S...

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Autores principales:	Carmen L Loureiro, Rossana C Jaspe, Pierina D Angelo, José L Zambrano, Lieska Rodriguez, Víctor Alarcon, Mariangel Delgado, Marwan Aguilar, Domingo Garzaro, Héctor R Rangel, Flor H Pujol
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ebfb8c503e92456d8b949f675ed4b10a
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spelling	oai:doaj.org-article:ebfb8c503e92456d8b949f675ed4b10a2021-12-02T20:18:56ZSARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.1932-620310.1371/journal.pone.0247196https://doaj.org/article/ebfb8c503e92456d8b949f675ed4b10a2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0247196https://doaj.org/toc/1932-6203SARS-CoV-2 is the new coronavirus responsible for COVID-19 disease. The first two cases of COVID-19 were detected in Venezuela on March 13, 2020. The aim of this study was the genetic characterization of Venezuelan SARS-CoV-2 isolates. A total of 7 full SARS-CoV-2 genome sequences were obtained by Sanger sequencing, from patients of different regions of Venezuela, mainly from the beginning of the epidemic. Ten out of 11 isolates (6 complete genomes and 4 partial spike genomic regions) belonged to lineage B, bearing the D614G mutation in the Spike protein. Isolates from the first outbreak that occurred in the Margarita Island harbored an in-frame deletion in its sequence, without amino acids 83-85 of the NSP1 of the ORF1. The search for deletions in 48,635 sequences showed that the NSP1 gene exhibit the highest frequency of deletions along the whole genome. Structural analysis suggests a change in the N-terminal domain with the presence of this deletion. In contrast, isolates circulating later in this island lacked the deletion, suggesting new introductions to the island after this first outbreak. In conclusion, a high diversity of SARS-CoV-2 isolates were found circulating in Venezuela, with predominance of the D614G mutation. The first small outbreak in Margarita Island seemed to be associated with a strain carrying a small deletion in the NSP1 protein, but these isolates do not seem to be responsible for the larger outbreak which started in July.Carmen L LoureiroRossana C JaspePierina D AngeloJosé L ZambranoLieska RodriguezVíctor AlarconMariangel DelgadoMarwan AguilarDomingo GarzaroHéctor R RangelFlor H PujolPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 2, p e0247196 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carmen L Loureiro Rossana C Jaspe Pierina D Angelo José L Zambrano Lieska Rodriguez Víctor Alarcon Mariangel Delgado Marwan Aguilar Domingo Garzaro Héctor R Rangel Flor H Pujol SARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.
description	SARS-CoV-2 is the new coronavirus responsible for COVID-19 disease. The first two cases of COVID-19 were detected in Venezuela on March 13, 2020. The aim of this study was the genetic characterization of Venezuelan SARS-CoV-2 isolates. A total of 7 full SARS-CoV-2 genome sequences were obtained by Sanger sequencing, from patients of different regions of Venezuela, mainly from the beginning of the epidemic. Ten out of 11 isolates (6 complete genomes and 4 partial spike genomic regions) belonged to lineage B, bearing the D614G mutation in the Spike protein. Isolates from the first outbreak that occurred in the Margarita Island harbored an in-frame deletion in its sequence, without amino acids 83-85 of the NSP1 of the ORF1. The search for deletions in 48,635 sequences showed that the NSP1 gene exhibit the highest frequency of deletions along the whole genome. Structural analysis suggests a change in the N-terminal domain with the presence of this deletion. In contrast, isolates circulating later in this island lacked the deletion, suggesting new introductions to the island after this first outbreak. In conclusion, a high diversity of SARS-CoV-2 isolates were found circulating in Venezuela, with predominance of the D614G mutation. The first small outbreak in Margarita Island seemed to be associated with a strain carrying a small deletion in the NSP1 protein, but these isolates do not seem to be responsible for the larger outbreak which started in July.
format	article
author	Carmen L Loureiro Rossana C Jaspe Pierina D Angelo José L Zambrano Lieska Rodriguez Víctor Alarcon Mariangel Delgado Marwan Aguilar Domingo Garzaro Héctor R Rangel Flor H Pujol
author_facet	Carmen L Loureiro Rossana C Jaspe Pierina D Angelo José L Zambrano Lieska Rodriguez Víctor Alarcon Mariangel Delgado Marwan Aguilar Domingo Garzaro Héctor R Rangel Flor H Pujol
author_sort	Carmen L Loureiro
title	SARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.
title_short	SARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.
title_full	SARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.
title_fullStr	SARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.
title_full_unstemmed	SARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.
title_sort	sars-cov-2 genetic diversity in venezuela: predominance of d614g variants and analysis of one outbreak.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ebfb8c503e92456d8b949f675ed4b10a
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SARS-CoV-2 genetic diversity in Venezuela: Predominance of D614G variants and analysis of one outbreak.

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