Bacteria Biofilms in Purulent-Septic Infections

The causative agents of many infectious diseases can exist in the form of biofilms. The aim of the work is to study of the frequency of occurrence and the degree of activity of biofilm formation of microorganisms isolated from different locus in purulent-septic infections.Materials and methods. Fift...

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Autores principales:	E. D. Savilov, E. V. Anganova, O. A. Noskova, A. V. Dukhanina
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2019
Materias:	bacteria biofilms purulent-septic infections locus of monitoring clinically important locus Science Q
Acceso en línea:	https://doaj.org/article/ebfcf0e131674713af808974d916cf43
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id	oai:doaj.org-article:ebfcf0e131674713af808974d916cf43
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spelling	oai:doaj.org-article:ebfcf0e131674713af808974d916cf432021-11-23T06:14:43ZBacteria Biofilms in Purulent-Septic Infections2541-94202587-959610.29413/ABS.2019-4.5.6https://doaj.org/article/ebfcf0e131674713af808974d916cf432019-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2166https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The causative agents of many infectious diseases can exist in the form of biofilms. The aim of the work is to study of the frequency of occurrence and the degree of activity of biofilm formation of microorganisms isolated from different locus in purulent-septic infections.Materials and methods. Fifteen strains isolated from patients with purulent-septic infections were examined. Biofilms were determined by the ability to adsorption a crystalviolet to ethanol.Results. 73,3 ± 11,4 % strains had biofilms (including gram-negative bacteria – 69,2 ± 11,9 %; Staphylococcus – 100,0 %; p < 0,05).The degree of activity of formation of biofilm by gram-negative bacteria was higher than Staphylococcus (0,302 ± 0,04 и 0,134 ± 0,01 units of optical density; p < 0,01). The highest activity of formation of biofilm was detected in K. pneumoniae isolated from patients with sepsis. Strains from clinically important locus (blood, sputum, wound discharge, abdominal fluid) had biofilms in 75,0 %; from locus of monitoring – 66,7 %. The pathogens isolated from locus of the monitoring were characterized by an average degree of activity of biofilm formation (0,180–0,360 units of optical density). Strains from clinically important locus (blood and sputum from patients with sepsis) had a highdegree of biofilm formation (more than 0,360 units of optical density). Conclusion. In most cases, strains were characterized by the presence of biofilms and differed in degrees activity of biofilm formation depending on locus.E. D. SavilovE. V. AnganovaO. A. NoskovaA. V. DukhaninaScientific Сentre for Family Health and Human Reproduction Problemsarticlebacteria biofilmspurulent-septic infectionslocus of monitoringclinically important locusScienceQRUActa Biomedica Scientifica, Vol 4, Iss 5, Pp 38-42 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	bacteria biofilms purulent-septic infections locus of monitoring clinically important locus Science Q
spellingShingle	bacteria biofilms purulent-septic infections locus of monitoring clinically important locus Science Q E. D. Savilov E. V. Anganova O. A. Noskova A. V. Dukhanina Bacteria Biofilms in Purulent-Septic Infections
description	The causative agents of many infectious diseases can exist in the form of biofilms. The aim of the work is to study of the frequency of occurrence and the degree of activity of biofilm formation of microorganisms isolated from different locus in purulent-septic infections.Materials and methods. Fifteen strains isolated from patients with purulent-septic infections were examined. Biofilms were determined by the ability to adsorption a crystalviolet to ethanol.Results. 73,3 ± 11,4 % strains had biofilms (including gram-negative bacteria – 69,2 ± 11,9 %; Staphylococcus – 100,0 %; p < 0,05).The degree of activity of formation of biofilm by gram-negative bacteria was higher than Staphylococcus (0,302 ± 0,04 и 0,134 ± 0,01 units of optical density; p < 0,01). The highest activity of formation of biofilm was detected in K. pneumoniae isolated from patients with sepsis. Strains from clinically important locus (blood, sputum, wound discharge, abdominal fluid) had biofilms in 75,0 %; from locus of monitoring – 66,7 %. The pathogens isolated from locus of the monitoring were characterized by an average degree of activity of biofilm formation (0,180–0,360 units of optical density). Strains from clinically important locus (blood and sputum from patients with sepsis) had a highdegree of biofilm formation (more than 0,360 units of optical density). Conclusion. In most cases, strains were characterized by the presence of biofilms and differed in degrees activity of biofilm formation depending on locus.
format	article
author	E. D. Savilov E. V. Anganova O. A. Noskova A. V. Dukhanina
author_facet	E. D. Savilov E. V. Anganova O. A. Noskova A. V. Dukhanina
author_sort	E. D. Savilov
title	Bacteria Biofilms in Purulent-Septic Infections
title_short	Bacteria Biofilms in Purulent-Septic Infections
title_full	Bacteria Biofilms in Purulent-Septic Infections
title_fullStr	Bacteria Biofilms in Purulent-Septic Infections
title_full_unstemmed	Bacteria Biofilms in Purulent-Septic Infections
title_sort	bacteria biofilms in purulent-septic infections
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2019
url	https://doaj.org/article/ebfcf0e131674713af808974d916cf43
work_keys_str_mv	AT edsavilov bacteriabiofilmsinpurulentsepticinfections AT evanganova bacteriabiofilmsinpurulentsepticinfections AT oanoskova bacteriabiofilmsinpurulentsepticinfections AT avdukhanina bacteriabiofilmsinpurulentsepticinfections
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Bacteria Biofilms in Purulent-Septic Infections

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