The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network

Abstract Background Ovarian serous cystadenocarcinoma is one of the most serious gynecological malignancies. Circular RNA (circRNA) is a type of noncoding RNA with a covalently closed continuous loop structure. Abnormal circRNA expression might be associated with tumorigenesis because of its complex...

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Autores principales:	Minhui Zhuang, Jian Zhao, Jing Wu, Shilong Fu, Ping Han, Xiaofeng Song
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	circRNA Expression profile Interaction network Ovarian serous cystadenocarcinoma Internal medicine RC31-1245 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/ebfde29b6a49496bbb9fd11a1498bb0a
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spelling	oai:doaj.org-article:ebfde29b6a49496bbb9fd11a1498bb0a2021-12-05T12:05:23ZThe circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network10.1186/s12920-021-01132-51755-8794https://doaj.org/article/ebfde29b6a49496bbb9fd11a1498bb0a2021-12-01T00:00:00Zhttps://doi.org/10.1186/s12920-021-01132-5https://doaj.org/toc/1755-8794Abstract Background Ovarian serous cystadenocarcinoma is one of the most serious gynecological malignancies. Circular RNA (circRNA) is a type of noncoding RNA with a covalently closed continuous loop structure. Abnormal circRNA expression might be associated with tumorigenesis because of its complex biological mechanisms by, for example, functioning as a microRNA (miRNA) sponge. However, the circRNA expression profile in ovarian serous cystadenocarcinoma and their associations with other RNAs have not yet been characterized. The main purpose of this study was to reveal the circRNA expression profile in ovarian serous cystadenocarcinoma. Methods We collected six specimens from three patients with ovarian serous cystadenocarcinoma and adjacent normal tissues. After RNA sequencing, we analyzed the expression of circRNAs with relevant mRNAs and miRNAs to characterize potential function. Results 15,092 unique circRNAs were identified in six specimens. Approximately 46% of these circRNAs were not recorded in public databases. We then reported 353 differentially expressed circRNAs with oncogenes and tumor-suppressor genes. Furthermore, a conjoint analysis with relevant mRNAs revealed consistent changes between circRNAs and their homologous mRNAs. Overall, construction of a circRNA–miRNA network suggested that 4 special circRNAs could be used as potential biomarkers. Conclusions Our study revealed the circRNA expression profile in the tissues of patients with ovarian serous cystadenocarcinoma. The differential expression of circRNAs was thought to be associated with ovarian serous cystadenocarcinoma in the enrichment analysis, and co-expression analysis with relevant mRNAs and miRNAs illustrated the latent regulatory network. We also constructed a complex circRNA–miRNA interaction network and then demonstrated the potential function of certain circRNAs to aid future diagnosis and treatment.Minhui ZhuangJian ZhaoJing WuShilong FuPing HanXiaofeng SongBMCarticlecircRNAExpression profileInteraction networkOvarian serous cystadenocarcinomaInternal medicineRC31-1245GeneticsQH426-470ENBMC Medical Genomics, Vol 14, Iss S2, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circRNA Expression profile Interaction network Ovarian serous cystadenocarcinoma Internal medicine RC31-1245 Genetics QH426-470
spellingShingle	circRNA Expression profile Interaction network Ovarian serous cystadenocarcinoma Internal medicine RC31-1245 Genetics QH426-470 Minhui Zhuang Jian Zhao Jing Wu Shilong Fu Ping Han Xiaofeng Song The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
description	Abstract Background Ovarian serous cystadenocarcinoma is one of the most serious gynecological malignancies. Circular RNA (circRNA) is a type of noncoding RNA with a covalently closed continuous loop structure. Abnormal circRNA expression might be associated with tumorigenesis because of its complex biological mechanisms by, for example, functioning as a microRNA (miRNA) sponge. However, the circRNA expression profile in ovarian serous cystadenocarcinoma and their associations with other RNAs have not yet been characterized. The main purpose of this study was to reveal the circRNA expression profile in ovarian serous cystadenocarcinoma. Methods We collected six specimens from three patients with ovarian serous cystadenocarcinoma and adjacent normal tissues. After RNA sequencing, we analyzed the expression of circRNAs with relevant mRNAs and miRNAs to characterize potential function. Results 15,092 unique circRNAs were identified in six specimens. Approximately 46% of these circRNAs were not recorded in public databases. We then reported 353 differentially expressed circRNAs with oncogenes and tumor-suppressor genes. Furthermore, a conjoint analysis with relevant mRNAs revealed consistent changes between circRNAs and their homologous mRNAs. Overall, construction of a circRNA–miRNA network suggested that 4 special circRNAs could be used as potential biomarkers. Conclusions Our study revealed the circRNA expression profile in the tissues of patients with ovarian serous cystadenocarcinoma. The differential expression of circRNAs was thought to be associated with ovarian serous cystadenocarcinoma in the enrichment analysis, and co-expression analysis with relevant mRNAs and miRNAs illustrated the latent regulatory network. We also constructed a complex circRNA–miRNA interaction network and then demonstrated the potential function of certain circRNAs to aid future diagnosis and treatment.
format	article
author	Minhui Zhuang Jian Zhao Jing Wu Shilong Fu Ping Han Xiaofeng Song
author_facet	Minhui Zhuang Jian Zhao Jing Wu Shilong Fu Ping Han Xiaofeng Song
author_sort	Minhui Zhuang
title	The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_short	The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_full	The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_fullStr	The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_full_unstemmed	The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network
title_sort	circular rna expression profile in ovarian serous cystadenocarcinoma reveals a complex circrna–mirna regulatory network
publisher	BMC
publishDate	2021
url	https://doaj.org/article/ebfde29b6a49496bbb9fd11a1498bb0a
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The circular RNA expression profile in ovarian serous cystadenocarcinoma reveals a complex circRNA–miRNA regulatory network

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