Early events during human coronavirus OC43 entry to the cell

Abstract The Coronaviridae family clusters a number of large RNA viruses, which share several structural and functional features. However, members of this family recognize different cellular receptors and exploit different entry routes, what affects their species specificity and virulence. The aim o...

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Autores principales:	Katarzyna Owczarek, Artur Szczepanski, Aleksandra Milewska, Zbigniew Baster, Zenon Rajfur, Michal Sarna, Krzysztof Pyrc
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec0c71fece9f4095b37cb4dc9265d111
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spelling	oai:doaj.org-article:ec0c71fece9f4095b37cb4dc9265d1112021-12-02T12:32:22ZEarly events during human coronavirus OC43 entry to the cell10.1038/s41598-018-25640-02045-2322https://doaj.org/article/ec0c71fece9f4095b37cb4dc9265d1112018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25640-0https://doaj.org/toc/2045-2322Abstract The Coronaviridae family clusters a number of large RNA viruses, which share several structural and functional features. However, members of this family recognize different cellular receptors and exploit different entry routes, what affects their species specificity and virulence. The aim of this study was to determine how human coronavirus OC43 enters the susceptible cell. Using confocal microscopy and molecular biology tools we visualized early events during infection. We found that the virus employs caveolin-1 dependent endocytosis for the entry and the scission of virus-containing vesicles from the cell surface is dynamin-dependent. Furthermore, the vesicle internalization process requires actin cytoskeleton rearrangements. With our research we strove to broaden the understanding of the infection process, which in future may be beneficial for the development of a potential therapeutics.Katarzyna OwczarekArtur SzczepanskiAleksandra MilewskaZbigniew BasterZenon RajfurMichal SarnaKrzysztof PyrcNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katarzyna Owczarek Artur Szczepanski Aleksandra Milewska Zbigniew Baster Zenon Rajfur Michal Sarna Krzysztof Pyrc Early events during human coronavirus OC43 entry to the cell
description	Abstract The Coronaviridae family clusters a number of large RNA viruses, which share several structural and functional features. However, members of this family recognize different cellular receptors and exploit different entry routes, what affects their species specificity and virulence. The aim of this study was to determine how human coronavirus OC43 enters the susceptible cell. Using confocal microscopy and molecular biology tools we visualized early events during infection. We found that the virus employs caveolin-1 dependent endocytosis for the entry and the scission of virus-containing vesicles from the cell surface is dynamin-dependent. Furthermore, the vesicle internalization process requires actin cytoskeleton rearrangements. With our research we strove to broaden the understanding of the infection process, which in future may be beneficial for the development of a potential therapeutics.
format	article
author	Katarzyna Owczarek Artur Szczepanski Aleksandra Milewska Zbigniew Baster Zenon Rajfur Michal Sarna Krzysztof Pyrc
author_facet	Katarzyna Owczarek Artur Szczepanski Aleksandra Milewska Zbigniew Baster Zenon Rajfur Michal Sarna Krzysztof Pyrc
author_sort	Katarzyna Owczarek
title	Early events during human coronavirus OC43 entry to the cell
title_short	Early events during human coronavirus OC43 entry to the cell
title_full	Early events during human coronavirus OC43 entry to the cell
title_fullStr	Early events during human coronavirus OC43 entry to the cell
title_full_unstemmed	Early events during human coronavirus OC43 entry to the cell
title_sort	early events during human coronavirus oc43 entry to the cell
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ec0c71fece9f4095b37cb4dc9265d111
work_keys_str_mv	AT katarzynaowczarek earlyeventsduringhumancoronavirusoc43entrytothecell AT arturszczepanski earlyeventsduringhumancoronavirusoc43entrytothecell AT aleksandramilewska earlyeventsduringhumancoronavirusoc43entrytothecell AT zbigniewbaster earlyeventsduringhumancoronavirusoc43entrytothecell AT zenonrajfur earlyeventsduringhumancoronavirusoc43entrytothecell AT michalsarna earlyeventsduringhumancoronavirusoc43entrytothecell AT krzysztofpyrc earlyeventsduringhumancoronavirusoc43entrytothecell
_version_	1718394129605459968

Early events during human coronavirus OC43 entry to the cell

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