Mitotic spindle orients perpendicular to the forces imposed by dynamic shear.

Orientation of the division axis can determine cell fate in the presence of morphogenetic gradients. Understanding how mitotic cells integrate directional cues is therefore an important question in embryogenesis. Here, we investigate the effect of dynamic shear forces on confined mitotic cells. We f...

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Autores principales:	Pablo Fernandez, Matthias Maier, Martina Lindauer, Christian Kuffer, Zuzana Storchova, Andreas R Bausch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec146e4250ff4031944d93488f9229f9
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spelling	oai:doaj.org-article:ec146e4250ff4031944d93488f9229f92021-11-18T07:31:18ZMitotic spindle orients perpendicular to the forces imposed by dynamic shear.1932-620310.1371/journal.pone.0028965https://doaj.org/article/ec146e4250ff4031944d93488f9229f92011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22220200/?tool=EBIhttps://doaj.org/toc/1932-6203Orientation of the division axis can determine cell fate in the presence of morphogenetic gradients. Understanding how mitotic cells integrate directional cues is therefore an important question in embryogenesis. Here, we investigate the effect of dynamic shear forces on confined mitotic cells. We found that human epithelial cells (hTERT-RPE1) as well as MC3T3 osteoblasts align their mitotic spindle perpendicular to the external force. Spindle orientation appears to be a consequence of cell elongation along the zero-force direction in response to the dynamic shear. This process is a nonlinear response to the strain amplitude, requires actomyosin activity and correlates with redistribution of myosin II. Mechanosteered cells divide normally, suggesting that this mechanism is compatible with biological functions.Pablo FernandezMatthias MaierMartina LindauerChristian KufferZuzana StorchovaAndreas R BauschPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28965 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pablo Fernandez Matthias Maier Martina Lindauer Christian Kuffer Zuzana Storchova Andreas R Bausch Mitotic spindle orients perpendicular to the forces imposed by dynamic shear.
description	Orientation of the division axis can determine cell fate in the presence of morphogenetic gradients. Understanding how mitotic cells integrate directional cues is therefore an important question in embryogenesis. Here, we investigate the effect of dynamic shear forces on confined mitotic cells. We found that human epithelial cells (hTERT-RPE1) as well as MC3T3 osteoblasts align their mitotic spindle perpendicular to the external force. Spindle orientation appears to be a consequence of cell elongation along the zero-force direction in response to the dynamic shear. This process is a nonlinear response to the strain amplitude, requires actomyosin activity and correlates with redistribution of myosin II. Mechanosteered cells divide normally, suggesting that this mechanism is compatible with biological functions.
format	article
author	Pablo Fernandez Matthias Maier Martina Lindauer Christian Kuffer Zuzana Storchova Andreas R Bausch
author_facet	Pablo Fernandez Matthias Maier Martina Lindauer Christian Kuffer Zuzana Storchova Andreas R Bausch
author_sort	Pablo Fernandez
title	Mitotic spindle orients perpendicular to the forces imposed by dynamic shear.
title_short	Mitotic spindle orients perpendicular to the forces imposed by dynamic shear.
title_full	Mitotic spindle orients perpendicular to the forces imposed by dynamic shear.
title_fullStr	Mitotic spindle orients perpendicular to the forces imposed by dynamic shear.
title_full_unstemmed	Mitotic spindle orients perpendicular to the forces imposed by dynamic shear.
title_sort	mitotic spindle orients perpendicular to the forces imposed by dynamic shear.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/ec146e4250ff4031944d93488f9229f9
work_keys_str_mv	AT pablofernandez mitoticspindleorientsperpendiculartotheforcesimposedbydynamicshear AT matthiasmaier mitoticspindleorientsperpendiculartotheforcesimposedbydynamicshear AT martinalindauer mitoticspindleorientsperpendiculartotheforcesimposedbydynamicshear AT christiankuffer mitoticspindleorientsperpendiculartotheforcesimposedbydynamicshear AT zuzanastorchova mitoticspindleorientsperpendiculartotheforcesimposedbydynamicshear AT andreasrbausch mitoticspindleorientsperpendiculartotheforcesimposedbydynamicshear
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Mitotic spindle orients perpendicular to the forces imposed by dynamic shear.

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