Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia

Abstract The protozoan parasite Giardia is a highly prevalent intestinal pathogen with a wide host range. Data obtained in mice, cattle and humans revealed the importance of IL-17A in the development of a protective immune response against Giardia. The aim of this study was to further unravel the pr...

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Autores principales:	Oonagh Paerewijck, Brecht Maertens, Leentje Dreesen, Frederik Van Meulder, Iris Peelaers, Dariusz Ratman, Robert W. Li, Erik Lubberts, Karolien De Bosscher, Peter Geldhof
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec19a01cba2d4ea98f63a20818ac59a8
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spelling	oai:doaj.org-article:ec19a01cba2d4ea98f63a20818ac59a82021-12-02T11:52:29ZInterleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia10.1038/s41598-017-08590-x2045-2322https://doaj.org/article/ec19a01cba2d4ea98f63a20818ac59a82017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08590-xhttps://doaj.org/toc/2045-2322Abstract The protozoan parasite Giardia is a highly prevalent intestinal pathogen with a wide host range. Data obtained in mice, cattle and humans revealed the importance of IL-17A in the development of a protective immune response against Giardia. The aim of this study was to further unravel the protective effector mechanisms triggered by IL-17A following G. muris infection in mice, by an RNA-sequencing approach. C57BL/6 WT and C57BL/6 IL-17RA KO mice were orally infected with G. muris cysts. Three weeks post infection, intestinal tissue samples were collected for RNA-sequencing, with samples from uninfected C57BL/6 WT and C57BL/6 IL-17RA KO animals serving as negative controls. Differential expression analysis showed that G. muris infection evoked the transcriptional upregulation of a wide array of genes, mainly in animals with competent IL-17RA signaling. IL-17RA signaling induced the production of various antimicrobial peptides, such as angiogenin 4 and α- and β-defensins and regulated complement activation through mannose-binding lectin 2. The expression of the receptor that regulates the secretion of IgA into the intestinal lumen, the polymeric immunoglobulin receptor, was also dependent on IL-17RA signaling. Interestingly, the transcriptome data showed for the first time the involvement of the circadian clock in the host response following Giardia infection.Oonagh PaerewijckBrecht MaertensLeentje DreesenFrederik Van MeulderIris PeelaersDariusz RatmanRobert W. LiErik LubbertsKarolien De BosscherPeter GeldhofNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oonagh Paerewijck Brecht Maertens Leentje Dreesen Frederik Van Meulder Iris Peelaers Dariusz Ratman Robert W. Li Erik Lubberts Karolien De Bosscher Peter Geldhof Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia
description	Abstract The protozoan parasite Giardia is a highly prevalent intestinal pathogen with a wide host range. Data obtained in mice, cattle and humans revealed the importance of IL-17A in the development of a protective immune response against Giardia. The aim of this study was to further unravel the protective effector mechanisms triggered by IL-17A following G. muris infection in mice, by an RNA-sequencing approach. C57BL/6 WT and C57BL/6 IL-17RA KO mice were orally infected with G. muris cysts. Three weeks post infection, intestinal tissue samples were collected for RNA-sequencing, with samples from uninfected C57BL/6 WT and C57BL/6 IL-17RA KO animals serving as negative controls. Differential expression analysis showed that G. muris infection evoked the transcriptional upregulation of a wide array of genes, mainly in animals with competent IL-17RA signaling. IL-17RA signaling induced the production of various antimicrobial peptides, such as angiogenin 4 and α- and β-defensins and regulated complement activation through mannose-binding lectin 2. The expression of the receptor that regulates the secretion of IgA into the intestinal lumen, the polymeric immunoglobulin receptor, was also dependent on IL-17RA signaling. Interestingly, the transcriptome data showed for the first time the involvement of the circadian clock in the host response following Giardia infection.
format	article
author	Oonagh Paerewijck Brecht Maertens Leentje Dreesen Frederik Van Meulder Iris Peelaers Dariusz Ratman Robert W. Li Erik Lubberts Karolien De Bosscher Peter Geldhof
author_facet	Oonagh Paerewijck Brecht Maertens Leentje Dreesen Frederik Van Meulder Iris Peelaers Dariusz Ratman Robert W. Li Erik Lubberts Karolien De Bosscher Peter Geldhof
author_sort	Oonagh Paerewijck
title	Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia
title_short	Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia
title_full	Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia
title_fullStr	Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia
title_full_unstemmed	Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia
title_sort	interleukin-17 receptor a (il-17ra) as a central regulator of the protective immune response against giardia
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ec19a01cba2d4ea98f63a20818ac59a8
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Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia

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