Comparative Genomics Analyses Support the Reclassification of Bisgaard Taxon 40 as Mergibacter gen. nov., With Mergibacter septicus sp. nov. as Type Species: Novel Insights Into the Phylogeny and Virulence Factors of a Pasteurellaceae Family Member Associated With Mortality Events in Seabirds

The Pasteurellaceae family has been associated with fatal diseases in numerous avian species. Several new taxa within this family, including Bisgaard taxon 40, have been recently described in wild birds, but their genomic characteristics and pathogenicity are not well understood. We isolated Bisgaar...

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Autores principales:	Eliana De Luca, Sonsiray Álvarez-Narváez, Grazieli Maboni, Rodrigo P. Baptista, Nicole M. Nemeth, Kevin D. Niedringhaus, Jason T. Ladner, Jeffrey M. Lorch, Galina Koroleva, Sean Lovett, Gustavo F. Palacios, Susan Sanchez
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bisgaard taxon 40 Pasteurellaceae genomic analysis evolution virulence factors phylogeny Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ec1ae2d87a154432b08fcb95fe82a167
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spelling	oai:doaj.org-article:ec1ae2d87a154432b08fcb95fe82a1672021-11-22T06:14:10ZComparative Genomics Analyses Support the Reclassification of Bisgaard Taxon 40 as Mergibacter gen. nov., With Mergibacter septicus sp. nov. as Type Species: Novel Insights Into the Phylogeny and Virulence Factors of a Pasteurellaceae Family Member Associated With Mortality Events in Seabirds1664-302X10.3389/fmicb.2021.667356https://doaj.org/article/ec1ae2d87a154432b08fcb95fe82a1672021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.667356/fullhttps://doaj.org/toc/1664-302XThe Pasteurellaceae family has been associated with fatal diseases in numerous avian species. Several new taxa within this family, including Bisgaard taxon 40, have been recently described in wild birds, but their genomic characteristics and pathogenicity are not well understood. We isolated Bisgaard taxon 40 from four species of seabirds, including one sampled during a mass, multi-species mortality event in Florida, United States. Here, we present a comprehensive phenotypic and genetic characterization of Bisgaard taxon 40 and comparative genomic analysis with reference strains from the Pasteurellaceae family, aiming at determining its phylogenetic position, antimicrobial susceptibility profile, and identifying putative virulence factors. In silico multilocus sequence-based and whole-genome-based phylogenetic analysis clustered all Bisgaard taxon 40 strains together on a distinct branch separated from the other members of the Pasteurellaceae family, indicating that Bisgaard taxon 40 could represent a new genus. These findings were further supported by protein similarity analyses using the concatenation of 31 conserved proteins and other taxonomic approaches such as the percentage of conserved protein test. Additionally, several putative virulence factors were identified, including those associated with adhesion (capsule, ompA, ompH) and colonization (exbD, fur, galU, galE, lpxA, lpxC, and kdsA) of the host and a cytolethal distending toxin (cdt), which may have played a role in disease development leading to the mortality event. Considerably low minimum inhibitory concentrations (MICs) were found for all the drugs tested, in concordance with the absence of antimicrobial resistance genes in these genomes. The novel findings of this study highlight genomic and phenotypic characteristics of this bacterium, providing insights into genome evolution and pathogenicity. We propose a reclassification of these organisms within the Pasteurellaceae family, designated as Mergibacter gen. nov., with Mergibacter septicus sp. nov. as the type species. The type strain is Mergibacter septicus A25201T (=DSM 112696).Eliana De LucaEliana De LucaSonsiray Álvarez-NarváezSonsiray Álvarez-NarváezGrazieli MaboniGrazieli MaboniGrazieli MaboniRodrigo P. BaptistaRodrigo P. BaptistaNicole M. NemethKevin D. NiedringhausKevin D. NiedringhausJason T. LadnerJason T. LadnerJeffrey M. LorchGalina KorolevaSean LovettGustavo F. PalaciosSusan SanchezSusan SanchezFrontiers Media S.A.articlebisgaard taxon 40Pasteurellaceaegenomic analysisevolutionvirulence factorsphylogenyMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bisgaard taxon 40 Pasteurellaceae genomic analysis evolution virulence factors phylogeny Microbiology QR1-502
spellingShingle	bisgaard taxon 40 Pasteurellaceae genomic analysis evolution virulence factors phylogeny Microbiology QR1-502 Eliana De Luca Eliana De Luca Sonsiray Álvarez-Narváez Sonsiray Álvarez-Narváez Grazieli Maboni Grazieli Maboni Grazieli Maboni Rodrigo P. Baptista Rodrigo P. Baptista Nicole M. Nemeth Kevin D. Niedringhaus Kevin D. Niedringhaus Jason T. Ladner Jason T. Ladner Jeffrey M. Lorch Galina Koroleva Sean Lovett Gustavo F. Palacios Susan Sanchez Susan Sanchez Comparative Genomics Analyses Support the Reclassification of Bisgaard Taxon 40 as Mergibacter gen. nov., With Mergibacter septicus sp. nov. as Type Species: Novel Insights Into the Phylogeny and Virulence Factors of a Pasteurellaceae Family Member Associated With Mortality Events in Seabirds
description	The Pasteurellaceae family has been associated with fatal diseases in numerous avian species. Several new taxa within this family, including Bisgaard taxon 40, have been recently described in wild birds, but their genomic characteristics and pathogenicity are not well understood. We isolated Bisgaard taxon 40 from four species of seabirds, including one sampled during a mass, multi-species mortality event in Florida, United States. Here, we present a comprehensive phenotypic and genetic characterization of Bisgaard taxon 40 and comparative genomic analysis with reference strains from the Pasteurellaceae family, aiming at determining its phylogenetic position, antimicrobial susceptibility profile, and identifying putative virulence factors. In silico multilocus sequence-based and whole-genome-based phylogenetic analysis clustered all Bisgaard taxon 40 strains together on a distinct branch separated from the other members of the Pasteurellaceae family, indicating that Bisgaard taxon 40 could represent a new genus. These findings were further supported by protein similarity analyses using the concatenation of 31 conserved proteins and other taxonomic approaches such as the percentage of conserved protein test. Additionally, several putative virulence factors were identified, including those associated with adhesion (capsule, ompA, ompH) and colonization (exbD, fur, galU, galE, lpxA, lpxC, and kdsA) of the host and a cytolethal distending toxin (cdt), which may have played a role in disease development leading to the mortality event. Considerably low minimum inhibitory concentrations (MICs) were found for all the drugs tested, in concordance with the absence of antimicrobial resistance genes in these genomes. The novel findings of this study highlight genomic and phenotypic characteristics of this bacterium, providing insights into genome evolution and pathogenicity. We propose a reclassification of these organisms within the Pasteurellaceae family, designated as Mergibacter gen. nov., with Mergibacter septicus sp. nov. as the type species. The type strain is Mergibacter septicus A25201T (=DSM 112696).
format	article
author	Eliana De Luca Eliana De Luca Sonsiray Álvarez-Narváez Sonsiray Álvarez-Narváez Grazieli Maboni Grazieli Maboni Grazieli Maboni Rodrigo P. Baptista Rodrigo P. Baptista Nicole M. Nemeth Kevin D. Niedringhaus Kevin D. Niedringhaus Jason T. Ladner Jason T. Ladner Jeffrey M. Lorch Galina Koroleva Sean Lovett Gustavo F. Palacios Susan Sanchez Susan Sanchez
author_facet	Eliana De Luca Eliana De Luca Sonsiray Álvarez-Narváez Sonsiray Álvarez-Narváez Grazieli Maboni Grazieli Maboni Grazieli Maboni Rodrigo P. Baptista Rodrigo P. Baptista Nicole M. Nemeth Kevin D. Niedringhaus Kevin D. Niedringhaus Jason T. Ladner Jason T. Ladner Jeffrey M. Lorch Galina Koroleva Sean Lovett Gustavo F. Palacios Susan Sanchez Susan Sanchez
author_sort	Eliana De Luca
title	Comparative Genomics Analyses Support the Reclassification of Bisgaard Taxon 40 as Mergibacter gen. nov., With Mergibacter septicus sp. nov. as Type Species: Novel Insights Into the Phylogeny and Virulence Factors of a Pasteurellaceae Family Member Associated With Mortality Events in Seabirds
title_short	Comparative Genomics Analyses Support the Reclassification of Bisgaard Taxon 40 as Mergibacter gen. nov., With Mergibacter septicus sp. nov. as Type Species: Novel Insights Into the Phylogeny and Virulence Factors of a Pasteurellaceae Family Member Associated With Mortality Events in Seabirds
title_full	Comparative Genomics Analyses Support the Reclassification of Bisgaard Taxon 40 as Mergibacter gen. nov., With Mergibacter septicus sp. nov. as Type Species: Novel Insights Into the Phylogeny and Virulence Factors of a Pasteurellaceae Family Member Associated With Mortality Events in Seabirds
title_fullStr	Comparative Genomics Analyses Support the Reclassification of Bisgaard Taxon 40 as Mergibacter gen. nov., With Mergibacter septicus sp. nov. as Type Species: Novel Insights Into the Phylogeny and Virulence Factors of a Pasteurellaceae Family Member Associated With Mortality Events in Seabirds
title_full_unstemmed	Comparative Genomics Analyses Support the Reclassification of Bisgaard Taxon 40 as Mergibacter gen. nov., With Mergibacter septicus sp. nov. as Type Species: Novel Insights Into the Phylogeny and Virulence Factors of a Pasteurellaceae Family Member Associated With Mortality Events in Seabirds
title_sort	comparative genomics analyses support the reclassification of bisgaard taxon 40 as mergibacter gen. nov., with mergibacter septicus sp. nov. as type species: novel insights into the phylogeny and virulence factors of a pasteurellaceae family member associated with mortality events in seabirds
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ec1ae2d87a154432b08fcb95fe82a167
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Comparative Genomics Analyses Support the Reclassification of Bisgaard Taxon 40 as Mergibacter gen. nov., With Mergibacter septicus sp. nov. as Type Species: Novel Insights Into the Phylogeny and Virulence Factors of a Pasteurellaceae Family Member Associated With Mortality Events in Seabirds

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