Exosomal lncRNA AK139128 Derived from Hypoxic Cardiomyocytes Promotes Apoptosis and Inhibits Cell Proliferation in Cardiac Fibroblasts

Lei Wang, Jun Zhang Cardiovascular Department, Cangzhou Central Hospital, Cangzhou, Hebei Province 061001, People’s Republic of ChinaCorrespondence: Jun Zhang Tel +86-13315777566Email 15245877343@163.comIntroduction: Myocardial infarction (MI) is the leading cause of congestive heart failu...

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Autores principales:	Wang L, Zhang J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	myocardial infarction hypoxia cardiomyocytes cardiac fibroblasts exosome lncrna ak139128 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ec1bbb999df44061a3012767a08157db
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spelling	oai:doaj.org-article:ec1bbb999df44061a3012767a08157db2021-12-02T11:39:19ZExosomal lncRNA AK139128 Derived from Hypoxic Cardiomyocytes Promotes Apoptosis and Inhibits Cell Proliferation in Cardiac Fibroblasts1178-2013https://doaj.org/article/ec1bbb999df44061a3012767a08157db2020-05-01T00:00:00Zhttps://www.dovepress.com/exosomal-lncrna-ak139128-derived-from-hypoxic-cardiomyocytes-promotes--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lei Wang, Jun Zhang Cardiovascular Department, Cangzhou Central Hospital, Cangzhou, Hebei Province 061001, People’s Republic of ChinaCorrespondence: Jun Zhang Tel +86-13315777566Email 15245877343@163.comIntroduction: Myocardial infarction (MI) is the leading cause of congestive heart failure and mortality. Hypoxia is an important trigger in the cardiac remodeling of the myocardium in the development and progression of cardiac diseases.Objective: Thus, we aimed to investigate the effect of hypoxia-induced exosomes on cardiac fibroblasts (CFs) and its related mechanisms.Materials and Methods: In this study, we successfully isolated and identified the exosomes from hypoxic cardiomyocytes (CMs). Exosomes derived from hypoxic CMs promoted apoptosis and inhibited proliferation, migration, and invasion in CFs. RNA-Seq assay suggested that long noncoding RNA AK139128 (lncRNA AK139128) was found to overexpress in both hypoxic CMs and CMs-secreting exosomes. After coculturing with CFs, hypoxic exosomes increased the expression of AK139128 in recipient CFs. Moreover, exosomal AK139128 derived from hypoxic CMs stimulated CFs apoptosis and inhibited proliferation, migration, and invasion. Furthermore, the effect of exosomal AK139128 derived from hypoxic CMs could also exacerbate MI in the rat model.Conclusion: Taken together, hypoxia upregulated the level of AK139128 in CMs and exosomes and exosomal AK139128 derived from hypoxic CMs modulated cellular activities of CFs in vitro and in vivo. This study provides a new understanding of the mechanism underlying hypoxia-related cardiac diseases and insight into developing new therapeutic strategies.Keywords: myocardial infarction, hypoxia, cardiomyocytes, cardiac fibroblasts, exosome, LncRNA AK139128Wang LZhang JDove Medical Pressarticlemyocardial infarctionhypoxiacardiomyocytescardiac fibroblastsexosomelncrna ak139128Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3363-3376 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	myocardial infarction hypoxia cardiomyocytes cardiac fibroblasts exosome lncrna ak139128 Medicine (General) R5-920
spellingShingle	myocardial infarction hypoxia cardiomyocytes cardiac fibroblasts exosome lncrna ak139128 Medicine (General) R5-920 Wang L Zhang J Exosomal lncRNA AK139128 Derived from Hypoxic Cardiomyocytes Promotes Apoptosis and Inhibits Cell Proliferation in Cardiac Fibroblasts
description	Lei Wang, Jun Zhang Cardiovascular Department, Cangzhou Central Hospital, Cangzhou, Hebei Province 061001, People’s Republic of ChinaCorrespondence: Jun Zhang Tel +86-13315777566Email 15245877343@163.comIntroduction: Myocardial infarction (MI) is the leading cause of congestive heart failure and mortality. Hypoxia is an important trigger in the cardiac remodeling of the myocardium in the development and progression of cardiac diseases.Objective: Thus, we aimed to investigate the effect of hypoxia-induced exosomes on cardiac fibroblasts (CFs) and its related mechanisms.Materials and Methods: In this study, we successfully isolated and identified the exosomes from hypoxic cardiomyocytes (CMs). Exosomes derived from hypoxic CMs promoted apoptosis and inhibited proliferation, migration, and invasion in CFs. RNA-Seq assay suggested that long noncoding RNA AK139128 (lncRNA AK139128) was found to overexpress in both hypoxic CMs and CMs-secreting exosomes. After coculturing with CFs, hypoxic exosomes increased the expression of AK139128 in recipient CFs. Moreover, exosomal AK139128 derived from hypoxic CMs stimulated CFs apoptosis and inhibited proliferation, migration, and invasion. Furthermore, the effect of exosomal AK139128 derived from hypoxic CMs could also exacerbate MI in the rat model.Conclusion: Taken together, hypoxia upregulated the level of AK139128 in CMs and exosomes and exosomal AK139128 derived from hypoxic CMs modulated cellular activities of CFs in vitro and in vivo. This study provides a new understanding of the mechanism underlying hypoxia-related cardiac diseases and insight into developing new therapeutic strategies.Keywords: myocardial infarction, hypoxia, cardiomyocytes, cardiac fibroblasts, exosome, LncRNA AK139128
format	article
author	Wang L Zhang J
author_facet	Wang L Zhang J
author_sort	Wang L
title	Exosomal lncRNA AK139128 Derived from Hypoxic Cardiomyocytes Promotes Apoptosis and Inhibits Cell Proliferation in Cardiac Fibroblasts
title_short	Exosomal lncRNA AK139128 Derived from Hypoxic Cardiomyocytes Promotes Apoptosis and Inhibits Cell Proliferation in Cardiac Fibroblasts
title_full	Exosomal lncRNA AK139128 Derived from Hypoxic Cardiomyocytes Promotes Apoptosis and Inhibits Cell Proliferation in Cardiac Fibroblasts
title_fullStr	Exosomal lncRNA AK139128 Derived from Hypoxic Cardiomyocytes Promotes Apoptosis and Inhibits Cell Proliferation in Cardiac Fibroblasts
title_full_unstemmed	Exosomal lncRNA AK139128 Derived from Hypoxic Cardiomyocytes Promotes Apoptosis and Inhibits Cell Proliferation in Cardiac Fibroblasts
title_sort	exosomal lncrna ak139128 derived from hypoxic cardiomyocytes promotes apoptosis and inhibits cell proliferation in cardiac fibroblasts
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/ec1bbb999df44061a3012767a08157db
work_keys_str_mv	AT wangl exosomallncrnaak139128derivedfromhypoxiccardiomyocytespromotesapoptosisandinhibitscellproliferationincardiacfibroblasts AT zhangj exosomallncrnaak139128derivedfromhypoxiccardiomyocytespromotesapoptosisandinhibitscellproliferationincardiacfibroblasts
_version_	1718395736463245312

Exosomal lncRNA AK139128 Derived from Hypoxic Cardiomyocytes Promotes Apoptosis and Inhibits Cell Proliferation in Cardiac Fibroblasts

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