Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines

Abstract. Lianah L, Khasanah RAN, Pranatami DA, Krisantini K. 2021. Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines. Biodiversitas 22: 913-919. The aim of the study was to analyze the phytochemical contents and todetermine the cytoto...

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Autores principales:	Lianah Lianah, RITA ARIYANA NUR KHASANAH, DWIMEI AYUDEWANDARI PRANATAMI, KRISANTINI KRISANTINI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	bauhinia, cytotoxicity, hela cells, phytochemicals, t47d cells Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ec1d973771aa4511888acc0cd92ad84d
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spelling	oai:doaj.org-article:ec1d973771aa4511888acc0cd92ad84d2021-11-22T00:54:56ZPhytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines1412-033X2085-472210.13057/biodiv/d220247https://doaj.org/article/ec1d973771aa4511888acc0cd92ad84d2021-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6954https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Lianah L, Khasanah RAN, Pranatami DA, Krisantini K. 2021. Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines. Biodiversitas 22: 913-919. The aim of the study was to analyze the phytochemical contents and todetermine the cytotoxic activities of Bauhinia scandens leaf ethanol extracts against HeLa (human cervical cancer) and T47D (breast cancer) cell culture lines. Using a standard protocol, it was found that leaf extract of B. scandens contains phytochemicals that are widely known to have medicinal properties, such as phenols (17.57±0.098% w/w), flavonoids (5.91±0.098% w/w), saponins (19.42±0.091% w/w), tannins (1.24±0.035% w/w), alkaloids (1.31±0.001% w/w) and steroids (0.08±0.007% w/w). Results of 24-hr Microculture Tetrazolium Salt (MTT) assay show that leaf extracts of B. scandens can suppress cell growth at a half-maximal inhibitory concentration (IC50) of 1.95 ?g/mLfor HeLa cells and 4.54?g/mL for T47D cells. This indicates that B. scandens is a potentially strong candidate for anti-cancer agents. The cytotoxic activities of the leaf extracts of B. scandens can be attributed to the total effects of the phytochemical compounds found. Further studies are recommended in order to isolate and determine individual effects of bioactive compounds in B. scandens.Lianah LianahRITA ARIYANA NUR KHASANAHDWIMEI AYUDEWANDARI PRANATAMIKRISANTINI KRISANTINIMBI & UNS Soloarticlebauhinia, cytotoxicity, hela cells, phytochemicals, t47d cellsBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bauhinia, cytotoxicity, hela cells, phytochemicals, t47d cells Biology (General) QH301-705.5
spellingShingle	bauhinia, cytotoxicity, hela cells, phytochemicals, t47d cells Biology (General) QH301-705.5 Lianah Lianah RITA ARIYANA NUR KHASANAH DWIMEI AYUDEWANDARI PRANATAMI KRISANTINI KRISANTINI Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines
description	Abstract. Lianah L, Khasanah RAN, Pranatami DA, Krisantini K. 2021. Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines. Biodiversitas 22: 913-919. The aim of the study was to analyze the phytochemical contents and todetermine the cytotoxic activities of Bauhinia scandens leaf ethanol extracts against HeLa (human cervical cancer) and T47D (breast cancer) cell culture lines. Using a standard protocol, it was found that leaf extract of B. scandens contains phytochemicals that are widely known to have medicinal properties, such as phenols (17.57±0.098% w/w), flavonoids (5.91±0.098% w/w), saponins (19.42±0.091% w/w), tannins (1.24±0.035% w/w), alkaloids (1.31±0.001% w/w) and steroids (0.08±0.007% w/w). Results of 24-hr Microculture Tetrazolium Salt (MTT) assay show that leaf extracts of B. scandens can suppress cell growth at a half-maximal inhibitory concentration (IC50) of 1.95 ?g/mLfor HeLa cells and 4.54?g/mL for T47D cells. This indicates that B. scandens is a potentially strong candidate for anti-cancer agents. The cytotoxic activities of the leaf extracts of B. scandens can be attributed to the total effects of the phytochemical compounds found. Further studies are recommended in order to isolate and determine individual effects of bioactive compounds in B. scandens.
format	article
author	Lianah Lianah RITA ARIYANA NUR KHASANAH DWIMEI AYUDEWANDARI PRANATAMI KRISANTINI KRISANTINI
author_facet	Lianah Lianah RITA ARIYANA NUR KHASANAH DWIMEI AYUDEWANDARI PRANATAMI KRISANTINI KRISANTINI
author_sort	Lianah Lianah
title	Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines
title_short	Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines
title_full	Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines
title_fullStr	Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines
title_full_unstemmed	Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines
title_sort	phytochemical screening and cytotoxic evaluation of bauhinia scandens leaf extracts using hela and t47d cell lines
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/ec1d973771aa4511888acc0cd92ad84d
work_keys_str_mv	AT lianahlianah phytochemicalscreeningandcytotoxicevaluationofbauhiniascandensleafextractsusinghelaandt47dcelllines AT ritaariyananurkhasanah phytochemicalscreeningandcytotoxicevaluationofbauhiniascandensleafextractsusinghelaandt47dcelllines AT dwimeiayudewandaripranatami phytochemicalscreeningandcytotoxicevaluationofbauhiniascandensleafextractsusinghelaandt47dcelllines AT krisantinikrisantini phytochemicalscreeningandcytotoxicevaluationofbauhiniascandensleafextractsusinghelaandt47dcelllines
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Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines

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