Persistence of SARS-CoV-2 infection on personal protective equipment (PPE)

Abstract Background SARS-CoV-2 stability and infection persistence has been studied on different surfaces, but scarce data exist related to personal protective equipment (PPE), moreover using realist viral loads for infection. Due to the importance for adequate PPE management to avoid risk of virus...

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Autores principales:	Elizabeth Córdoba-Lanús, Omar García-Pérez, Sara Cazorla-Rivero, Francisco Rodríguez-Esparragón, José-Enrique Piñero, Bernardino Clavo, Jacob Lorenzo-Morales
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	COVID-19 SARS-CoV-2 RNA PPE Personal protective equipment Face masks Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/ec20bf21d993413490128adde2cbba8a
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spelling	oai:doaj.org-article:ec20bf21d993413490128adde2cbba8a2021-11-21T12:42:36ZPersistence of SARS-CoV-2 infection on personal protective equipment (PPE)10.1186/s12879-021-06861-71471-2334https://doaj.org/article/ec20bf21d993413490128adde2cbba8a2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12879-021-06861-7https://doaj.org/toc/1471-2334Abstract Background SARS-CoV-2 stability and infection persistence has been studied on different surfaces, but scarce data exist related to personal protective equipment (PPE), moreover using realist viral loads for infection. Due to the importance for adequate PPE management to avoid risk of virus infection, RNA stability was evaluated on PPE. Methods Persistence of SARS-CoV-2 infection and detection of genomic RNA in PPE (gowns and face masks) were determined by in-vitro assays and RT-qPCR, respectively. Samples were infected with a clinical sample positive for SARS-CoV-2 (Clin-Inf), and with a heat-inactivated SARS-CoV-2 strain sample (Str-Inf) as a control. Results PPE samples infected with Clin-Inf were positive for the 3 viral genes on gowns up to 5 days post-infection, whereas these overall genes were detected up to 30 days in the case of face masks. However, gowns and FFP2 masks samples contaminated with Clin-Inf showed a cytopathic effect over VERO cells up to 5–7 days post-infection. Conclusions SARS-CoV-2 RNA was detected on different PPE materials for 5 to 30 days, but PPE contaminated with the virus was infectious up to 5–7 days. These findings demonstrate the need to improve PPE management and to formulate strategies to introduce viricidal compounds in PPE fabrics.Elizabeth Córdoba-LanúsOmar García-PérezSara Cazorla-RiveroFrancisco Rodríguez-EsparragónJosé-Enrique PiñeroBernardino ClavoJacob Lorenzo-MoralesBMCarticleCOVID-19SARS-CoV-2RNAPPEPersonal protective equipmentFace masksInfectious and parasitic diseasesRC109-216ENBMC Infectious Diseases, Vol 21, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 SARS-CoV-2 RNA PPE Personal protective equipment Face masks Infectious and parasitic diseases RC109-216
spellingShingle	COVID-19 SARS-CoV-2 RNA PPE Personal protective equipment Face masks Infectious and parasitic diseases RC109-216 Elizabeth Córdoba-Lanús Omar García-Pérez Sara Cazorla-Rivero Francisco Rodríguez-Esparragón José-Enrique Piñero Bernardino Clavo Jacob Lorenzo-Morales Persistence of SARS-CoV-2 infection on personal protective equipment (PPE)
description	Abstract Background SARS-CoV-2 stability and infection persistence has been studied on different surfaces, but scarce data exist related to personal protective equipment (PPE), moreover using realist viral loads for infection. Due to the importance for adequate PPE management to avoid risk of virus infection, RNA stability was evaluated on PPE. Methods Persistence of SARS-CoV-2 infection and detection of genomic RNA in PPE (gowns and face masks) were determined by in-vitro assays and RT-qPCR, respectively. Samples were infected with a clinical sample positive for SARS-CoV-2 (Clin-Inf), and with a heat-inactivated SARS-CoV-2 strain sample (Str-Inf) as a control. Results PPE samples infected with Clin-Inf were positive for the 3 viral genes on gowns up to 5 days post-infection, whereas these overall genes were detected up to 30 days in the case of face masks. However, gowns and FFP2 masks samples contaminated with Clin-Inf showed a cytopathic effect over VERO cells up to 5–7 days post-infection. Conclusions SARS-CoV-2 RNA was detected on different PPE materials for 5 to 30 days, but PPE contaminated with the virus was infectious up to 5–7 days. These findings demonstrate the need to improve PPE management and to formulate strategies to introduce viricidal compounds in PPE fabrics.
format	article
author	Elizabeth Córdoba-Lanús Omar García-Pérez Sara Cazorla-Rivero Francisco Rodríguez-Esparragón José-Enrique Piñero Bernardino Clavo Jacob Lorenzo-Morales
author_facet	Elizabeth Córdoba-Lanús Omar García-Pérez Sara Cazorla-Rivero Francisco Rodríguez-Esparragón José-Enrique Piñero Bernardino Clavo Jacob Lorenzo-Morales
author_sort	Elizabeth Córdoba-Lanús
title	Persistence of SARS-CoV-2 infection on personal protective equipment (PPE)
title_short	Persistence of SARS-CoV-2 infection on personal protective equipment (PPE)
title_full	Persistence of SARS-CoV-2 infection on personal protective equipment (PPE)
title_fullStr	Persistence of SARS-CoV-2 infection on personal protective equipment (PPE)
title_full_unstemmed	Persistence of SARS-CoV-2 infection on personal protective equipment (PPE)
title_sort	persistence of sars-cov-2 infection on personal protective equipment (ppe)
publisher	BMC
publishDate	2021
url	https://doaj.org/article/ec20bf21d993413490128adde2cbba8a
work_keys_str_mv	AT elizabethcordobalanus persistenceofsarscov2infectiononpersonalprotectiveequipmentppe AT omargarciaperez persistenceofsarscov2infectiononpersonalprotectiveequipmentppe AT saracazorlarivero persistenceofsarscov2infectiononpersonalprotectiveequipmentppe AT franciscorodriguezesparragon persistenceofsarscov2infectiononpersonalprotectiveequipmentppe AT joseenriquepinero persistenceofsarscov2infectiononpersonalprotectiveequipmentppe AT bernardinoclavo persistenceofsarscov2infectiononpersonalprotectiveequipmentppe AT jacoblorenzomorales persistenceofsarscov2infectiononpersonalprotectiveequipmentppe
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Persistence of SARS-CoV-2 infection on personal protective equipment (PPE)

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