Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog

Kohei Kawamoto,1 Hirofumi Miyaji,1 Erika Nishida,1 Saori Miyata,1 Akihito Kato,1 Akito Tateyama,1 Tomokazu Furihata,1 Kanako Shitomi,1 Toshihiko Iwanaga,2 Tsutomu Sugaya1 1Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan; 2Department of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kawamoto K, Miyaji H, Nishida E, Miyata S, Kato A, Tateyama A, Furihata T, Shitomi K, Iwanaga T, Sugaya T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Collagen scaffold cytotoxicity graphene oxide (GO) periodontal attachment rat cranial bone augmentation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ec259aa160a34139958cdc00e0aa17d2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ec259aa160a34139958cdc00e0aa17d2
record_format	dspace
spelling	oai:doaj.org-article:ec259aa160a34139958cdc00e0aa17d22021-12-02T07:41:11ZCharacterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog1178-2013https://doaj.org/article/ec259aa160a34139958cdc00e0aa17d22018-04-01T00:00:00Zhttps://www.dovepress.com/characterization-and-evaluation-of-graphene-oxide-scaffold-for-periodo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Kohei Kawamoto,1 Hirofumi Miyaji,1 Erika Nishida,1 Saori Miyata,1 Akihito Kato,1 Akito Tateyama,1 Tomokazu Furihata,1 Kanako Shitomi,1 Toshihiko Iwanaga,2 Tsutomu Sugaya1 1Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan; 2Department of Histology and Cytology, Faculty of Medicine, Hokkaido University, Sapporo, Japan Introduction: The 3-dimensional scaffold plays a key role in volume and quality of repair tissue in periodontal tissue engineering therapy. We fabricated a novel 3D collagen scaffold containing carbon-based 2-dimensional layered material, named graphene oxide (GO). The aim of this study was to characterize and assess GO scaffold for periodontal tissue healing of class II furcation defects in dog.Materials and methods: GO scaffolds were prepared by coating the surface of a 3D collagen sponge scaffold with GO dispersion. Scaffolds were characterized using cytotoxicity and tissue reactivity tests. In addition, GO scaffold was implanted into dog class II furcation defects and periodontal healing was investigated at 4 weeks postsurgery.Results: GO scaffold exhibited low cytotoxicity and enhanced cellular ingrowth behavior and rat bone forming ability. In addition, GO scaffold stimulated healing of dog class II furcation defects. Periodontal attachment formation, including alveolar bone, periodontal ligament-like tissue, and cementum-like tissue, was significantly increased by GO scaffold implantation, compared with untreated scaffold.Conclusion: The results suggest that GO scaffold is biocompatible and possesses excellent bone and periodontal tissue formation ability. Therefore, GO scaffold would be beneficial for periodontal tissue engineering therapy. Keywords: artificial collagen scaffold, cytotoxicity, nanocarbon, periodontal attachment, periodontal tissue engineering, rat cranial bone augmentation Kawamoto KMiyaji HNishida EMiyata SKato ATateyama AFurihata TShitomi KIwanaga TSugaya TDove Medical PressarticleCollagen scaffoldcytotoxicitygraphene oxide (GO)periodontal attachmentrat cranial bone augmentationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 2365-2376 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Collagen scaffold cytotoxicity graphene oxide (GO) periodontal attachment rat cranial bone augmentation Medicine (General) R5-920
spellingShingle	Collagen scaffold cytotoxicity graphene oxide (GO) periodontal attachment rat cranial bone augmentation Medicine (General) R5-920 Kawamoto K Miyaji H Nishida E Miyata S Kato A Tateyama A Furihata T Shitomi K Iwanaga T Sugaya T Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog
description	Kohei Kawamoto,1 Hirofumi Miyaji,1 Erika Nishida,1 Saori Miyata,1 Akihito Kato,1 Akito Tateyama,1 Tomokazu Furihata,1 Kanako Shitomi,1 Toshihiko Iwanaga,2 Tsutomu Sugaya1 1Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan; 2Department of Histology and Cytology, Faculty of Medicine, Hokkaido University, Sapporo, Japan Introduction: The 3-dimensional scaffold plays a key role in volume and quality of repair tissue in periodontal tissue engineering therapy. We fabricated a novel 3D collagen scaffold containing carbon-based 2-dimensional layered material, named graphene oxide (GO). The aim of this study was to characterize and assess GO scaffold for periodontal tissue healing of class II furcation defects in dog.Materials and methods: GO scaffolds were prepared by coating the surface of a 3D collagen sponge scaffold with GO dispersion. Scaffolds were characterized using cytotoxicity and tissue reactivity tests. In addition, GO scaffold was implanted into dog class II furcation defects and periodontal healing was investigated at 4 weeks postsurgery.Results: GO scaffold exhibited low cytotoxicity and enhanced cellular ingrowth behavior and rat bone forming ability. In addition, GO scaffold stimulated healing of dog class II furcation defects. Periodontal attachment formation, including alveolar bone, periodontal ligament-like tissue, and cementum-like tissue, was significantly increased by GO scaffold implantation, compared with untreated scaffold.Conclusion: The results suggest that GO scaffold is biocompatible and possesses excellent bone and periodontal tissue formation ability. Therefore, GO scaffold would be beneficial for periodontal tissue engineering therapy. Keywords: artificial collagen scaffold, cytotoxicity, nanocarbon, periodontal attachment, periodontal tissue engineering, rat cranial bone augmentation
format	article
author	Kawamoto K Miyaji H Nishida E Miyata S Kato A Tateyama A Furihata T Shitomi K Iwanaga T Sugaya T
author_facet	Kawamoto K Miyaji H Nishida E Miyata S Kato A Tateyama A Furihata T Shitomi K Iwanaga T Sugaya T
author_sort	Kawamoto K
title	Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog
title_short	Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog
title_full	Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog
title_fullStr	Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog
title_full_unstemmed	Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog
title_sort	characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class ii furcation defects in dog
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/ec259aa160a34139958cdc00e0aa17d2
work_keys_str_mv	AT kawamotok characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog AT miyajih characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog AT nishidae characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog AT miyatas characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog AT katoa characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog AT tateyamaa characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog AT furihatat characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog AT shitomik characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog AT iwanagat characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog AT sugayat characterizationandevaluationofgrapheneoxidescaffoldforperiodontalwoundhealingofclassiifurcationdefectsindog
_version_	1718399313769398272

Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog

Ejemplares similares