Choosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria

The article presents results of research related to the selection of an effective method for isolating bacteriophages active against bacteria of the genus Flavobacterium. The number of bacterial strains used in the work — 3. All strains cultures were obtained from the museum of the department of mic...

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Autores principales:	A. G. Semanin, G. R. Sadrtdinova
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	bacteria bacteriophages isolation method morphology lytic activity Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/ec2713e134f64463903e73d27fe3feeb
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spelling	oai:doaj.org-article:ec2713e134f64463903e73d27fe3feeb2021-11-22T07:09:53ZChoosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria2220-76192313-739810.15789/2220-7619-2019-2-399-403https://doaj.org/article/ec2713e134f64463903e73d27fe3feeb2019-07-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/656https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The article presents results of research related to the selection of an effective method for isolating bacteriophages active against bacteria of the genus Flavobacterium. The number of bacterial strains used in the work — 3. All strains cultures were obtained from the museum of the department of microbiology, virology, epizootiology and veterinary-sanitary examination of federal state budgetary educational institution of higher education Ulyanovsk «State Agricultural University named after PA Stolypin» and have tinctorial and biochemical properties characteristic for this genus (and species). The studies consisted of 4 series of experiments. The first series of experiments was connected with the approbation of a technique for isolating a bacteriophage without using an influencing factor. As a result, the desired bacteriophages were not isolated. The second series of experiments consisted in testing the isolation method using an inducing factorultraviolet irradiation. The distance to the objects of irradiation is0.3 m. Exposure time: 1 min, 3 min, 5 min, 10 min, 15 min. The third series of experiments was also associated with the use of inducing factor X-ray irradiation. In the course of the work, three operating modes were determined, each of which differed in the duration of the exposure, the periodicity of the exposure, and the dose received. As a result of studies related to the use of inducing factors, the desired bacteriophages were not identified. The last series of experiments was to allocate bacteriophages from the objects of the natural places сommemorative (possible) existence of the «owner» (the studied microorganism) and viruses (bacteriophage desired). Three phage isolates were isolated from environmental objects: FL-j-1, FL-pec-2, FL-aq-3. Bacteriophage FL-j-1 is active against a strain of F. johnsoniae VKM B-1426 bacteriophage FL-pec-2 is active against strain F. pectinovorum VKM B-1171 bacteriophage FL-aq-3 strain active against F. aquatile VKPM B-8534. Further studies may be related to study properties of the isolated phages: study of the specificity in respect of this kind of sensitivity study, the study and higher lytic activity, selection of the most promising strains phages further selection of selected phages, as well as studying the possibility of bacteriophages from use in the method of the reaction increase titer phage for the purpose of indicating the microorganism being studied.A. G. SemaninG. R. SadrtdinovaSankt-Peterburg : NIIÈM imeni Pasteraarticlebacteriabacteriophagesisolationmethodmorphologylytic activityInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 2, Pp 399-403 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	bacteria bacteriophages isolation method morphology lytic activity Infectious and parasitic diseases RC109-216
spellingShingle	bacteria bacteriophages isolation method morphology lytic activity Infectious and parasitic diseases RC109-216 A. G. Semanin G. R. Sadrtdinova Choosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria
description	The article presents results of research related to the selection of an effective method for isolating bacteriophages active against bacteria of the genus Flavobacterium. The number of bacterial strains used in the work — 3. All strains cultures were obtained from the museum of the department of microbiology, virology, epizootiology and veterinary-sanitary examination of federal state budgetary educational institution of higher education Ulyanovsk «State Agricultural University named after PA Stolypin» and have tinctorial and biochemical properties characteristic for this genus (and species). The studies consisted of 4 series of experiments. The first series of experiments was connected with the approbation of a technique for isolating a bacteriophage without using an influencing factor. As a result, the desired bacteriophages were not isolated. The second series of experiments consisted in testing the isolation method using an inducing factorultraviolet irradiation. The distance to the objects of irradiation is0.3 m. Exposure time: 1 min, 3 min, 5 min, 10 min, 15 min. The third series of experiments was also associated with the use of inducing factor X-ray irradiation. In the course of the work, three operating modes were determined, each of which differed in the duration of the exposure, the periodicity of the exposure, and the dose received. As a result of studies related to the use of inducing factors, the desired bacteriophages were not identified. The last series of experiments was to allocate bacteriophages from the objects of the natural places сommemorative (possible) existence of the «owner» (the studied microorganism) and viruses (bacteriophage desired). Three phage isolates were isolated from environmental objects: FL-j-1, FL-pec-2, FL-aq-3. Bacteriophage FL-j-1 is active against a strain of F. johnsoniae VKM B-1426 bacteriophage FL-pec-2 is active against strain F. pectinovorum VKM B-1171 bacteriophage FL-aq-3 strain active against F. aquatile VKPM B-8534. Further studies may be related to study properties of the isolated phages: study of the specificity in respect of this kind of sensitivity study, the study and higher lytic activity, selection of the most promising strains phages further selection of selected phages, as well as studying the possibility of bacteriophages from use in the method of the reaction increase titer phage for the purpose of indicating the microorganism being studied.
format	article
author	A. G. Semanin G. R. Sadrtdinova
author_facet	A. G. Semanin G. R. Sadrtdinova
author_sort	A. G. Semanin
title	Choosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria
title_short	Choosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria
title_full	Choosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria
title_fullStr	Choosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria
title_full_unstemmed	Choosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria
title_sort	choosing a proper approach for isolating bacteriophages specific to flavobacterium genus bacteria
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/ec2713e134f64463903e73d27fe3feeb
work_keys_str_mv	AT agsemanin choosingaproperapproachforisolatingbacteriophagesspecifictoflavobacteriumgenusbacteria AT grsadrtdinova choosingaproperapproachforisolatingbacteriophagesspecifictoflavobacteriumgenusbacteria
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Choosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria

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