Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing

Abstract Ceramide (Cer) release from glucosylceramides (GlcCer) is critical for the formation of the skin permeability barrier. Changes in β-glucocerebrosidase (GlcCer’ase) activity lead to diminished Cer, GlcCer accumulation and structural defects in SC lipid lamellae; however, the molecular basis...

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Autores principales:	Michaela Sochorová, Klára Staňková, Petra Pullmannová, Andrej Kováčik, Jarmila Zbytovská, Kateřina Vávrová
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec2786da6eb34f7a8a0b6fe5e380cd79
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spelling	oai:doaj.org-article:ec2786da6eb34f7a8a0b6fe5e380cd792021-12-02T16:08:10ZPermeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing10.1038/s41598-017-06990-72045-2322https://doaj.org/article/ec2786da6eb34f7a8a0b6fe5e380cd792017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06990-7https://doaj.org/toc/2045-2322Abstract Ceramide (Cer) release from glucosylceramides (GlcCer) is critical for the formation of the skin permeability barrier. Changes in β-glucocerebrosidase (GlcCer’ase) activity lead to diminished Cer, GlcCer accumulation and structural defects in SC lipid lamellae; however, the molecular basis for this impairment is not clear. We investigated impaired GlcCer-to-Cer processing in human Cer membranes to determine the physicochemical properties responsible for the barrier defects. Minor impairment (5–25%) of the Cer generation from GlcCer decreased the permeability of the model membrane to four markers and altered the membrane microstructure (studied by X-ray powder diffraction and infrared spectroscopy), in agreement with the effects of topical GlcCer in human skin. At these concentrations, the accumulation of GlcCer was a stronger contributor to this disturbance than the lack of human Cer. However, replacement of 50–100% human Cer by GlcCer led to the formation of a new lamellar phase and the maintenance of a rather good barrier to the four studied permeability markers. These findings suggest that the major cause of the impaired water permeability barrier in complete GlcCer’ase deficiency is not the accumulation of free GlcCer but other factors, possibly the retention of GlcCer bound in the corneocyte lipid envelope.Michaela SochorováKlára StaňkováPetra PullmannováAndrej KováčikJarmila ZbytovskáKateřina VávrováNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michaela Sochorová Klára Staňková Petra Pullmannová Andrej Kováčik Jarmila Zbytovská Kateřina Vávrová Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing
description	Abstract Ceramide (Cer) release from glucosylceramides (GlcCer) is critical for the formation of the skin permeability barrier. Changes in β-glucocerebrosidase (GlcCer’ase) activity lead to diminished Cer, GlcCer accumulation and structural defects in SC lipid lamellae; however, the molecular basis for this impairment is not clear. We investigated impaired GlcCer-to-Cer processing in human Cer membranes to determine the physicochemical properties responsible for the barrier defects. Minor impairment (5–25%) of the Cer generation from GlcCer decreased the permeability of the model membrane to four markers and altered the membrane microstructure (studied by X-ray powder diffraction and infrared spectroscopy), in agreement with the effects of topical GlcCer in human skin. At these concentrations, the accumulation of GlcCer was a stronger contributor to this disturbance than the lack of human Cer. However, replacement of 50–100% human Cer by GlcCer led to the formation of a new lamellar phase and the maintenance of a rather good barrier to the four studied permeability markers. These findings suggest that the major cause of the impaired water permeability barrier in complete GlcCer’ase deficiency is not the accumulation of free GlcCer but other factors, possibly the retention of GlcCer bound in the corneocyte lipid envelope.
format	article
author	Michaela Sochorová Klára Staňková Petra Pullmannová Andrej Kováčik Jarmila Zbytovská Kateřina Vávrová
author_facet	Michaela Sochorová Klára Staňková Petra Pullmannová Andrej Kováčik Jarmila Zbytovská Kateřina Vávrová
author_sort	Michaela Sochorová
title	Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing
title_short	Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing
title_full	Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing
title_fullStr	Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing
title_full_unstemmed	Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing
title_sort	permeability barrier and microstructure of skin lipid membrane models of impaired glucosylceramide processing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ec2786da6eb34f7a8a0b6fe5e380cd79
work_keys_str_mv	AT michaelasochorova permeabilitybarrierandmicrostructureofskinlipidmembranemodelsofimpairedglucosylceramideprocessing AT klarastankova permeabilitybarrierandmicrostructureofskinlipidmembranemodelsofimpairedglucosylceramideprocessing AT petrapullmannova permeabilitybarrierandmicrostructureofskinlipidmembranemodelsofimpairedglucosylceramideprocessing AT andrejkovacik permeabilitybarrierandmicrostructureofskinlipidmembranemodelsofimpairedglucosylceramideprocessing AT jarmilazbytovska permeabilitybarrierandmicrostructureofskinlipidmembranemodelsofimpairedglucosylceramideprocessing AT katerinavavrova permeabilitybarrierandmicrostructureofskinlipidmembranemodelsofimpairedglucosylceramideprocessing
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Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing

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