Attenuator LRR – a regulatory tool for modulating gene expression in Gram‐positive bacteria

Summary With the rapid development of synthetic biology in recent years, particular attention has been paid to RNA devices, especially riboswitches, because of their significant and diverse regulatory roles in prokaryotic and eukaryotic cells. Due to the limited performance and context‐dependence of...

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Autores principales:	Xia Cai, Qian Wang, Yu Fang, Die Yao, Yunda Zhan, Baoju An, Bing Yan, Jun Cai
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/ec2fdd9ea1d244ae87d4f2401a8e9af6
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spelling	oai:doaj.org-article:ec2fdd9ea1d244ae87d4f2401a8e9af62021-11-18T15:39:53ZAttenuator LRR – a regulatory tool for modulating gene expression in Gram‐positive bacteria1751-791510.1111/1751-7915.13797https://doaj.org/article/ec2fdd9ea1d244ae87d4f2401a8e9af62021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13797https://doaj.org/toc/1751-7915Summary With the rapid development of synthetic biology in recent years, particular attention has been paid to RNA devices, especially riboswitches, because of their significant and diverse regulatory roles in prokaryotic and eukaryotic cells. Due to the limited performance and context‐dependence of riboswitches, only a few of them (such as theophylline, tetracycline and ciprofloxacin riboswitches) have been utilized as regulatory tools in biotechnology. In the present study, we demonstrated that a ribosome‐dependent ribo‐regulator, LRR, discovered in our previous work, exhibits an attractive regulatory performance. Specifically, it offers a 60‐fold change in expression in the presence of retapamulin and a low level of leaky expression of about 1–2% without antibiotics. Moreover, LRR can be combined with different promoters and performs well in Bacillus thuringiensis, B. cereus, B. amyloliquefaciens, and B. subtilis. Additionally, LRR also functions in the Gram‐negative bacterium Escherichia coli. Furthermore, we demonstrate its ability to control melanin metabolism in B. thuringiensis BMB171. Our results show that LRR can be applied to regulate gene expression, construct genetic circuits and tune metabolic pathways, and has great potential for many applications in synthetic biology.Xia CaiQian WangYu FangDie YaoYunda ZhanBaoju AnBing YanJun CaiWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2538-2551 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Xia Cai Qian Wang Yu Fang Die Yao Yunda Zhan Baoju An Bing Yan Jun Cai Attenuator LRR – a regulatory tool for modulating gene expression in Gram‐positive bacteria
description	Summary With the rapid development of synthetic biology in recent years, particular attention has been paid to RNA devices, especially riboswitches, because of their significant and diverse regulatory roles in prokaryotic and eukaryotic cells. Due to the limited performance and context‐dependence of riboswitches, only a few of them (such as theophylline, tetracycline and ciprofloxacin riboswitches) have been utilized as regulatory tools in biotechnology. In the present study, we demonstrated that a ribosome‐dependent ribo‐regulator, LRR, discovered in our previous work, exhibits an attractive regulatory performance. Specifically, it offers a 60‐fold change in expression in the presence of retapamulin and a low level of leaky expression of about 1–2% without antibiotics. Moreover, LRR can be combined with different promoters and performs well in Bacillus thuringiensis, B. cereus, B. amyloliquefaciens, and B. subtilis. Additionally, LRR also functions in the Gram‐negative bacterium Escherichia coli. Furthermore, we demonstrate its ability to control melanin metabolism in B. thuringiensis BMB171. Our results show that LRR can be applied to regulate gene expression, construct genetic circuits and tune metabolic pathways, and has great potential for many applications in synthetic biology.
format	article
author	Xia Cai Qian Wang Yu Fang Die Yao Yunda Zhan Baoju An Bing Yan Jun Cai
author_facet	Xia Cai Qian Wang Yu Fang Die Yao Yunda Zhan Baoju An Bing Yan Jun Cai
author_sort	Xia Cai
title	Attenuator LRR – a regulatory tool for modulating gene expression in Gram‐positive bacteria
title_short	Attenuator LRR – a regulatory tool for modulating gene expression in Gram‐positive bacteria
title_full	Attenuator LRR – a regulatory tool for modulating gene expression in Gram‐positive bacteria
title_fullStr	Attenuator LRR – a regulatory tool for modulating gene expression in Gram‐positive bacteria
title_full_unstemmed	Attenuator LRR – a regulatory tool for modulating gene expression in Gram‐positive bacteria
title_sort	attenuator lrr – a regulatory tool for modulating gene expression in gram‐positive bacteria
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/ec2fdd9ea1d244ae87d4f2401a8e9af6
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Attenuator LRR – a regulatory tool for modulating gene expression in Gram‐positive bacteria

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