Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity

Genome editing technologies enable the rapid interrogation of genetic alterations. Here, the authors present a CRISPR/Cas9-based platform to simultaneously investigate multiple activating point mutations in de novo cancers in mice; and generate panels of Kras-variants in different tissues to induce...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ian P. Winters, Shin-Heng Chiou, Nicole K. Paulk, Christopher D. McFarland, Pranav V. Lalgudi, Rosanna K. Ma, Leszek Lisowski, Andrew J. Connolly, Dmitri A. Petrov, Mark A. Kay, Monte M. Winslow
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ec34a2e663bb4e9aa7d634750cb16aee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ec34a2e663bb4e9aa7d634750cb16aee
record_format	dspace
spelling	oai:doaj.org-article:ec34a2e663bb4e9aa7d634750cb16aee2021-12-02T14:40:34ZMultiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity10.1038/s41467-017-01519-y2041-1723https://doaj.org/article/ec34a2e663bb4e9aa7d634750cb16aee2017-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01519-yhttps://doaj.org/toc/2041-1723Genome editing technologies enable the rapid interrogation of genetic alterations. Here, the authors present a CRISPR/Cas9-based platform to simultaneously investigate multiple activating point mutations in de novo cancers in mice; and generate panels of Kras-variants in different tissues to induce cancer.Ian P. WintersShin-Heng ChiouNicole K. PaulkChristopher D. McFarlandPranav V. LalgudiRosanna K. MaLeszek LisowskiAndrew J. ConnollyDmitri A. PetrovMark A. KayMonte M. WinslowNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ian P. Winters Shin-Heng Chiou Nicole K. Paulk Christopher D. McFarland Pranav V. Lalgudi Rosanna K. Ma Leszek Lisowski Andrew J. Connolly Dmitri A. Petrov Mark A. Kay Monte M. Winslow Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity
description	Genome editing technologies enable the rapid interrogation of genetic alterations. Here, the authors present a CRISPR/Cas9-based platform to simultaneously investigate multiple activating point mutations in de novo cancers in mice; and generate panels of Kras-variants in different tissues to induce cancer.
format	article
author	Ian P. Winters Shin-Heng Chiou Nicole K. Paulk Christopher D. McFarland Pranav V. Lalgudi Rosanna K. Ma Leszek Lisowski Andrew J. Connolly Dmitri A. Petrov Mark A. Kay Monte M. Winslow
author_facet	Ian P. Winters Shin-Heng Chiou Nicole K. Paulk Christopher D. McFarland Pranav V. Lalgudi Rosanna K. Ma Leszek Lisowski Andrew J. Connolly Dmitri A. Petrov Mark A. Kay Monte M. Winslow
author_sort	Ian P. Winters
title	Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity
title_short	Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity
title_full	Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity
title_fullStr	Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity
title_full_unstemmed	Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity
title_sort	multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of kras variant oncogenicity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ec34a2e663bb4e9aa7d634750cb16aee
work_keys_str_mv	AT ianpwinters multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT shinhengchiou multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT nicolekpaulk multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT christopherdmcfarland multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT pranavvlalgudi multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT rosannakma multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT leszeklisowski multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT andrewjconnolly multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT dmitriapetrov multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT markakay multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity AT montemwinslow multiplexedinvivohomologydirectedrepairandtumorbarcodingenablesparallelquantificationofkrasvariantoncogenicity
_version_	1718390270492409856

Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity

Ejemplares similares