Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect

Critical defects of the mandibular bone are very difficult to manage with currently available materials and technology. In the present work, we generated acellular and cellular substitutes for human bone by tissue engineering using nanostructured fibrin-agarose biomaterials, with and without adipose...

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Autores principales:	Miguel-Angel Martin-Piedra, Belén Gironés-Camarasa, Antonio España-López, Ricardo Fernández-Valadés Gámez, Cristina Blanco-Elices, Ingrid Garzón, Miguel Alaminos, Ricardo Fernández-Valadés
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bone tissue engineering fibrin-agarose mandible regeneration Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/ec35f419b26c4e8c8f8bd7607e2e06ce
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spelling	oai:doaj.org-article:ec35f419b26c4e8c8f8bd7607e2e06ce2021-11-25T18:48:30ZUsefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect10.3390/polym132239392073-4360https://doaj.org/article/ec35f419b26c4e8c8f8bd7607e2e06ce2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3939https://doaj.org/toc/2073-4360Critical defects of the mandibular bone are very difficult to manage with currently available materials and technology. In the present work, we generated acellular and cellular substitutes for human bone by tissue engineering using nanostructured fibrin-agarose biomaterials, with and without adipose-tissue-derived mesenchymal stem cells differentiated to the osteogenic lineage using inductive media. Then, these substitutes were evaluated in an immunodeficient animal model of severely critical mandibular bone damage in order to assess the potential of the bioartificial tissues to enable bone regeneration. The results showed that the use of a cellular bone substitute was associated with a morpho-functional improvement of maxillofacial structures as compared to negative controls. Analysis of the defect site showed that none of the study groups fully succeeded in generating dense bone tissue at the regeneration area. However, the use of a cellular substitute was able to improve the density of the regenerated tissue (as determined via CT radiodensity) and form isolated islands of bone and cartilage. Histologically, the regenerated bone islands were comparable to control bone for alizarin red and versican staining, and superior to control bone for toluidine blue and osteocalcin in animals grafted with the cellular substitute. Although these results are preliminary, cellular fibrin-agarose bone substitutes show preliminary signs of usefulness in this animal model of severely critical mandibular bone defect.Miguel-Angel Martin-PiedraBelén Gironés-CamarasaAntonio España-LópezRicardo Fernández-Valadés GámezCristina Blanco-ElicesIngrid GarzónMiguel AlaminosRicardo Fernández-ValadésMDPI AGarticlebonetissue engineeringfibrin-agarosemandibleregenerationOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3939, p 3939 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bone tissue engineering fibrin-agarose mandible regeneration Organic chemistry QD241-441
spellingShingle	bone tissue engineering fibrin-agarose mandible regeneration Organic chemistry QD241-441 Miguel-Angel Martin-Piedra Belén Gironés-Camarasa Antonio España-López Ricardo Fernández-Valadés Gámez Cristina Blanco-Elices Ingrid Garzón Miguel Alaminos Ricardo Fernández-Valadés Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect
description	Critical defects of the mandibular bone are very difficult to manage with currently available materials and technology. In the present work, we generated acellular and cellular substitutes for human bone by tissue engineering using nanostructured fibrin-agarose biomaterials, with and without adipose-tissue-derived mesenchymal stem cells differentiated to the osteogenic lineage using inductive media. Then, these substitutes were evaluated in an immunodeficient animal model of severely critical mandibular bone damage in order to assess the potential of the bioartificial tissues to enable bone regeneration. The results showed that the use of a cellular bone substitute was associated with a morpho-functional improvement of maxillofacial structures as compared to negative controls. Analysis of the defect site showed that none of the study groups fully succeeded in generating dense bone tissue at the regeneration area. However, the use of a cellular substitute was able to improve the density of the regenerated tissue (as determined via CT radiodensity) and form isolated islands of bone and cartilage. Histologically, the regenerated bone islands were comparable to control bone for alizarin red and versican staining, and superior to control bone for toluidine blue and osteocalcin in animals grafted with the cellular substitute. Although these results are preliminary, cellular fibrin-agarose bone substitutes show preliminary signs of usefulness in this animal model of severely critical mandibular bone defect.
format	article
author	Miguel-Angel Martin-Piedra Belén Gironés-Camarasa Antonio España-López Ricardo Fernández-Valadés Gámez Cristina Blanco-Elices Ingrid Garzón Miguel Alaminos Ricardo Fernández-Valadés
author_facet	Miguel-Angel Martin-Piedra Belén Gironés-Camarasa Antonio España-López Ricardo Fernández-Valadés Gámez Cristina Blanco-Elices Ingrid Garzón Miguel Alaminos Ricardo Fernández-Valadés
author_sort	Miguel-Angel Martin-Piedra
title	Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect
title_short	Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect
title_full	Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect
title_fullStr	Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect
title_full_unstemmed	Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect
title_sort	usefulness of a nanostructured fibrin-agarose bone substitute in a model of severely critical mandible bone defect
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ec35f419b26c4e8c8f8bd7607e2e06ce
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Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect

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