Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9

Abstract Coat color is an important characteristic and economic trait in domestic sheep. Aiming at alteration of Chinese merino sheep coat color by genome manipulation, we disrupted sheep agouti signaling protein gene by CRISPR/Cas9. A total of seven indels were identified in 5 of 6 born lambs. Each...

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Autores principales:	Xuemei Zhang, Wenrong Li, Chenxi Liu, Xinrong Peng, Jiapeng Lin, Sangang He, Xuejiao Li, Bing Han, Ning Zhang, Yangsheng Wu, Lei Chen, Liqin Wang, MaYila, Juncheng Huang, Mingjun Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec41b019e6a04b4885e4dc74851bba6f
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spelling	oai:doaj.org-article:ec41b019e6a04b4885e4dc74851bba6f2021-12-02T12:31:47ZAlteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas910.1038/s41598-017-08636-02045-2322https://doaj.org/article/ec41b019e6a04b4885e4dc74851bba6f2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08636-0https://doaj.org/toc/2045-2322Abstract Coat color is an important characteristic and economic trait in domestic sheep. Aiming at alteration of Chinese merino sheep coat color by genome manipulation, we disrupted sheep agouti signaling protein gene by CRISPR/Cas9. A total of seven indels were identified in 5 of 6 born lambs. Each targeted lamb happened at least two kinds of modifications, and targeted lambs with multiple modifications displayed variety of coat color patterns. Three lambs with 4 bp deletion showed badgerface with black body coat color in two lambs, and brown coat color with light ventral pigmentation in another one. The black-white spotted color was observed in two lambs with 2 bp deletion. Further analysis unraveled that modifications happened in one or more than two copies of ASIP gene, and moreover, the additional spontaneous mutations of D9 and/or D5 preceding the targeting modification could also involve the formation of coat color patterns. Taken together, the entanglement of ASIP modifications by CRISPR/Cas9, spontaneous D9/D5 mutations, and ASIP gene duplications contributed to the variety of coat color patterns in targeted lambs.Xuemei ZhangWenrong LiChenxi LiuXinrong PengJiapeng LinSangang HeXuejiao LiBing HanNing ZhangYangsheng WuLei ChenLiqin WangMaYilaJuncheng HuangMingjun LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xuemei Zhang Wenrong Li Chenxi Liu Xinrong Peng Jiapeng Lin Sangang He Xuejiao Li Bing Han Ning Zhang Yangsheng Wu Lei Chen Liqin Wang MaYila Juncheng Huang Mingjun Liu Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9
description	Abstract Coat color is an important characteristic and economic trait in domestic sheep. Aiming at alteration of Chinese merino sheep coat color by genome manipulation, we disrupted sheep agouti signaling protein gene by CRISPR/Cas9. A total of seven indels were identified in 5 of 6 born lambs. Each targeted lamb happened at least two kinds of modifications, and targeted lambs with multiple modifications displayed variety of coat color patterns. Three lambs with 4 bp deletion showed badgerface with black body coat color in two lambs, and brown coat color with light ventral pigmentation in another one. The black-white spotted color was observed in two lambs with 2 bp deletion. Further analysis unraveled that modifications happened in one or more than two copies of ASIP gene, and moreover, the additional spontaneous mutations of D9 and/or D5 preceding the targeting modification could also involve the formation of coat color patterns. Taken together, the entanglement of ASIP modifications by CRISPR/Cas9, spontaneous D9/D5 mutations, and ASIP gene duplications contributed to the variety of coat color patterns in targeted lambs.
format	article
author	Xuemei Zhang Wenrong Li Chenxi Liu Xinrong Peng Jiapeng Lin Sangang He Xuejiao Li Bing Han Ning Zhang Yangsheng Wu Lei Chen Liqin Wang MaYila Juncheng Huang Mingjun Liu
author_facet	Xuemei Zhang Wenrong Li Chenxi Liu Xinrong Peng Jiapeng Lin Sangang He Xuejiao Li Bing Han Ning Zhang Yangsheng Wu Lei Chen Liqin Wang MaYila Juncheng Huang Mingjun Liu
author_sort	Xuemei Zhang
title	Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9
title_short	Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9
title_full	Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9
title_fullStr	Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9
title_full_unstemmed	Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9
title_sort	alteration of sheep coat color pattern by disruption of asip gene via crispr cas9
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ec41b019e6a04b4885e4dc74851bba6f
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Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9

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