Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.

Long noncoding RNAs (lncRNAs) are vastly transcribed and extensively studied but lncRNAs overlapping with the sense orientation of mRNA have been poorly studied. We analyzed the lncRNA DAPALR overlapping with the 5´ UTR of the Doublesex1 (Dsx1), the male determining gene in Daphnia magna. By affinit...

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Autores principales:	Christelle Alexa Garcia Perez, Shungo Adachi, Quang Dang Nong, Nikko Adhitama, Tomoaki Matsuura, Toru Natsume, Tadashi Wada, Yasuhiko Kato, Hajime Watanabe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/ec4349c4d921424abb7251efb732e657
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spelling	oai:doaj.org-article:ec4349c4d921424abb7251efb732e6572021-12-02T20:02:54ZSense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.1553-73901553-740410.1371/journal.pgen.1009683https://doaj.org/article/ec4349c4d921424abb7251efb732e6572021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009683https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Long noncoding RNAs (lncRNAs) are vastly transcribed and extensively studied but lncRNAs overlapping with the sense orientation of mRNA have been poorly studied. We analyzed the lncRNA DAPALR overlapping with the 5´ UTR of the Doublesex1 (Dsx1), the male determining gene in Daphnia magna. By affinity purification, we identified an RNA binding protein, Shep as a DAPALR binding protein. Shep also binds to Dsx1 5´ UTR by recognizing the overlapping sequence and suppresses translation of the mRNA. In vitro and in vivo analyses indicated that DAPALR increased Dsx1 translation efficiency by sequestration of Shep. This regulation was impaired when the Shep binding site in DAPALR was deleted. These results suggest that Shep suppresses the unintentional translation of Dsx1 by setting a threshold; and when the sense lncRNA DAPALR is expressed, DAPALR cancels the suppression caused by Shep. This mechanism may be important to show dimorphic gene expressions such as sex determination and it may account for the binary expression in various developmental processes.Christelle Alexa Garcia PerezShungo AdachiQuang Dang NongNikko AdhitamaTomoaki MatsuuraToru NatsumeTadashi WadaYasuhiko KatoHajime WatanabePublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 7, p e1009683 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Christelle Alexa Garcia Perez Shungo Adachi Quang Dang Nong Nikko Adhitama Tomoaki Matsuura Toru Natsume Tadashi Wada Yasuhiko Kato Hajime Watanabe Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.
description	Long noncoding RNAs (lncRNAs) are vastly transcribed and extensively studied but lncRNAs overlapping with the sense orientation of mRNA have been poorly studied. We analyzed the lncRNA DAPALR overlapping with the 5´ UTR of the Doublesex1 (Dsx1), the male determining gene in Daphnia magna. By affinity purification, we identified an RNA binding protein, Shep as a DAPALR binding protein. Shep also binds to Dsx1 5´ UTR by recognizing the overlapping sequence and suppresses translation of the mRNA. In vitro and in vivo analyses indicated that DAPALR increased Dsx1 translation efficiency by sequestration of Shep. This regulation was impaired when the Shep binding site in DAPALR was deleted. These results suggest that Shep suppresses the unintentional translation of Dsx1 by setting a threshold; and when the sense lncRNA DAPALR is expressed, DAPALR cancels the suppression caused by Shep. This mechanism may be important to show dimorphic gene expressions such as sex determination and it may account for the binary expression in various developmental processes.
format	article
author	Christelle Alexa Garcia Perez Shungo Adachi Quang Dang Nong Nikko Adhitama Tomoaki Matsuura Toru Natsume Tadashi Wada Yasuhiko Kato Hajime Watanabe
author_facet	Christelle Alexa Garcia Perez Shungo Adachi Quang Dang Nong Nikko Adhitama Tomoaki Matsuura Toru Natsume Tadashi Wada Yasuhiko Kato Hajime Watanabe
author_sort	Christelle Alexa Garcia Perez
title	Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.
title_short	Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.
title_full	Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.
title_fullStr	Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.
title_full_unstemmed	Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.
title_sort	sense-overlapping lncrna as a decoy of translational repressor protein for dimorphic gene expression.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ec4349c4d921424abb7251efb732e657
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Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression.

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