Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in ce...
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Nature Portfolio
2021
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oai:doaj.org-article:ec5522b1496a4c3d90ef8ee39e17ce5a2021-12-02T19:16:51ZUnraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas910.1038/s41467-021-25991-92041-1723https://doaj.org/article/ec5522b1496a4c3d90ef8ee39e17ce5a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25991-9https://doaj.org/toc/2041-1723The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in cells and tackle this question.Daniel M. SapozhnikovMoshe SzyfNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-26 (2021) |
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Science Q Daniel M. Sapozhnikov Moshe Szyf Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9 |
description |
The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in cells and tackle this question. |
format |
article |
author |
Daniel M. Sapozhnikov Moshe Szyf |
author_facet |
Daniel M. Sapozhnikov Moshe Szyf |
author_sort |
Daniel M. Sapozhnikov |
title |
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9 |
title_short |
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9 |
title_full |
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9 |
title_fullStr |
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9 |
title_full_unstemmed |
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9 |
title_sort |
unraveling the functional role of dna demethylation at specific promoters by targeted steric blockage of dna methyltransferase with crispr/dcas9 |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/ec5522b1496a4c3d90ef8ee39e17ce5a |
work_keys_str_mv |
AT danielmsapozhnikov unravelingthefunctionalroleofdnademethylationatspecificpromotersbytargetedstericblockageofdnamethyltransferasewithcrisprdcas9 AT mosheszyf unravelingthefunctionalroleofdnademethylationatspecificpromotersbytargetedstericblockageofdnamethyltransferasewithcrisprdcas9 |
_version_ |
1718376946367201280 |