Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9

The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in ce...

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Autores principales: Daniel M. Sapozhnikov, Moshe Szyf
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/ec5522b1496a4c3d90ef8ee39e17ce5a
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spelling oai:doaj.org-article:ec5522b1496a4c3d90ef8ee39e17ce5a2021-12-02T19:16:51ZUnraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas910.1038/s41467-021-25991-92041-1723https://doaj.org/article/ec5522b1496a4c3d90ef8ee39e17ce5a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25991-9https://doaj.org/toc/2041-1723The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in cells and tackle this question.Daniel M. SapozhnikovMoshe SzyfNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-26 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Daniel M. Sapozhnikov
Moshe Szyf
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
description The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in cells and tackle this question.
format article
author Daniel M. Sapozhnikov
Moshe Szyf
author_facet Daniel M. Sapozhnikov
Moshe Szyf
author_sort Daniel M. Sapozhnikov
title Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_short Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_full Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_fullStr Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_full_unstemmed Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_sort unraveling the functional role of dna demethylation at specific promoters by targeted steric blockage of dna methyltransferase with crispr/dcas9
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/ec5522b1496a4c3d90ef8ee39e17ce5a
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AT mosheszyf unravelingthefunctionalroleofdnademethylationatspecificpromotersbytargetedstericblockageofdnamethyltransferasewithcrisprdcas9
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