PRNP haplotype associated with classical BSE incidence in European Holstein cattle.

<h4>Background</h4>Classical bovine spongiform encephalopathy (BSE) is an acquired prion disease of cattle. The bovine prion gene (PRNP) contains regions of both high and low linkage disequilibrium (LD) that appear to be conserved across Bos taurus populations. The region of high LD, whi...

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Autores principales:	Brenda M Murdoch, Michael L Clawson, Samuel Yue, Urmila Basu, Stephanie McKay, Matthew Settles, Rossana Capoferri, William W Laegreid, John L Williams, Stephen S Moore
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec5a75bfa9eb4991990ca8159a553ae8
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spelling	oai:doaj.org-article:ec5a75bfa9eb4991990ca8159a553ae82021-11-18T06:35:06ZPRNP haplotype associated with classical BSE incidence in European Holstein cattle.1932-620310.1371/journal.pone.0012786https://doaj.org/article/ec5a75bfa9eb4991990ca8159a553ae82010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20862290/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Classical bovine spongiform encephalopathy (BSE) is an acquired prion disease of cattle. The bovine prion gene (PRNP) contains regions of both high and low linkage disequilibrium (LD) that appear to be conserved across Bos taurus populations. The region of high LD, which spans the promoter and part of intron 2, contains polymorphic loci that have been associated with classical BSE status. However, the complex genetic architecture of PRNP has not been systematically tested for an association with classical BSE.<h4>Methodology/principal findings</h4>In this study, haplotype tagging single nucleotide polymorphisms (htSNPs) within PRNP were used to test for association between PRNP haplotypes and BSE disease. A combination of Illumina goldengate assay, sequencing and PCR amplification was used to genotype 18 htSNPs and 2 indels in 95 BSE case and 134 control animals. A haplotype within the region of high LD was found to be associated with BSE unaffected animals (p-value=0.000114).<h4>Conclusion/significance</h4>A PRNP haplotype association with classical BSE incidence has been identified. This result suggests that a genetic determinant in or near PRNP may influence classical BSE incidence in cattle.Brenda M MurdochMichael L ClawsonSamuel YueUrmila BasuStephanie McKayMatthew SettlesRossana CapoferriWilliam W LaegreidJohn L WilliamsStephen S MoorePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brenda M Murdoch Michael L Clawson Samuel Yue Urmila Basu Stephanie McKay Matthew Settles Rossana Capoferri William W Laegreid John L Williams Stephen S Moore PRNP haplotype associated with classical BSE incidence in European Holstein cattle.
description	<h4>Background</h4>Classical bovine spongiform encephalopathy (BSE) is an acquired prion disease of cattle. The bovine prion gene (PRNP) contains regions of both high and low linkage disequilibrium (LD) that appear to be conserved across Bos taurus populations. The region of high LD, which spans the promoter and part of intron 2, contains polymorphic loci that have been associated with classical BSE status. However, the complex genetic architecture of PRNP has not been systematically tested for an association with classical BSE.<h4>Methodology/principal findings</h4>In this study, haplotype tagging single nucleotide polymorphisms (htSNPs) within PRNP were used to test for association between PRNP haplotypes and BSE disease. A combination of Illumina goldengate assay, sequencing and PCR amplification was used to genotype 18 htSNPs and 2 indels in 95 BSE case and 134 control animals. A haplotype within the region of high LD was found to be associated with BSE unaffected animals (p-value=0.000114).<h4>Conclusion/significance</h4>A PRNP haplotype association with classical BSE incidence has been identified. This result suggests that a genetic determinant in or near PRNP may influence classical BSE incidence in cattle.
format	article
author	Brenda M Murdoch Michael L Clawson Samuel Yue Urmila Basu Stephanie McKay Matthew Settles Rossana Capoferri William W Laegreid John L Williams Stephen S Moore
author_facet	Brenda M Murdoch Michael L Clawson Samuel Yue Urmila Basu Stephanie McKay Matthew Settles Rossana Capoferri William W Laegreid John L Williams Stephen S Moore
author_sort	Brenda M Murdoch
title	PRNP haplotype associated with classical BSE incidence in European Holstein cattle.
title_short	PRNP haplotype associated with classical BSE incidence in European Holstein cattle.
title_full	PRNP haplotype associated with classical BSE incidence in European Holstein cattle.
title_fullStr	PRNP haplotype associated with classical BSE incidence in European Holstein cattle.
title_full_unstemmed	PRNP haplotype associated with classical BSE incidence in European Holstein cattle.
title_sort	prnp haplotype associated with classical bse incidence in european holstein cattle.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/ec5a75bfa9eb4991990ca8159a553ae8
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PRNP haplotype associated with classical BSE incidence in European Holstein cattle.

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