Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population

Abstract Glucoraphanin is a major secondary metabolite found in Brassicaceae vegetables, especially broccoli, and its degradation product sulforaphane plays an essential role in anticancer. The fine mapping of sulforaphane metabolism quantitative trait loci (QTLs) in broccoli florets is necessary fo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhansheng Li, Yumei Liu, Suxia Yuan, Fengqing Han, Zhiyuan Fang, Limei Yang, Mu Zhuang, Yangyong Zhang, Honghao Lv, Yong Wang, Jialei Ji
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec66e8092a7a4f0db1f6ac1cdc488561
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ec66e8092a7a4f0db1f6ac1cdc488561
record_format	dspace
spelling	oai:doaj.org-article:ec66e8092a7a4f0db1f6ac1cdc4885612021-12-02T17:39:20ZFine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population10.1038/s41598-021-88652-32045-2322https://doaj.org/article/ec66e8092a7a4f0db1f6ac1cdc4885612021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88652-3https://doaj.org/toc/2045-2322Abstract Glucoraphanin is a major secondary metabolite found in Brassicaceae vegetables, especially broccoli, and its degradation product sulforaphane plays an essential role in anticancer. The fine mapping of sulforaphane metabolism quantitative trait loci (QTLs) in broccoli florets is necessary for future marker-assisted selection strategies. In this study, we utilized a doubled haploid population consisting of 176 lines derived from two inbred lines (86,101 and 90,196) with significant differences in sulforaphane content, coupled with extensive genotypic and phenotypic data from two independent environments. A linkage map consisting of 438 simple sequence repeats markers was constructed, covering a length of 1168.26 cM. A total of 18 QTLs for sulforaphane metabolism in broccoli florets were detected, 10 were detected in 2017, and the other 8 were detected in 2018. The LOD values of all QTLs ranged from 3.06 to 14.47, explaining 1.74–7.03% of the biochemical variation between two years. Finally, 6 QTLs (qSF-C3-1, qSF-C3-2, qSF-C3-3, qSF-C3-5, qSF-C3-6 and qSF-C7) were stably detected in more than one environment, each accounting for 4.54–7.03% of the phenotypic variation explained (PVE) and a total of 30.88–34.86% of PVE. Our study provides new insights into sulforaphane metabolism in broccoli florets and marker-assisted selection breeding in Brassica oleracea crops.Zhansheng LiYumei LiuSuxia YuanFengqing HanZhiyuan FangLimei YangMu ZhuangYangyong ZhangHonghao LvYong WangJialei JiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhansheng Li Yumei Liu Suxia Yuan Fengqing Han Zhiyuan Fang Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Yong Wang Jialei Ji Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population
description	Abstract Glucoraphanin is a major secondary metabolite found in Brassicaceae vegetables, especially broccoli, and its degradation product sulforaphane plays an essential role in anticancer. The fine mapping of sulforaphane metabolism quantitative trait loci (QTLs) in broccoli florets is necessary for future marker-assisted selection strategies. In this study, we utilized a doubled haploid population consisting of 176 lines derived from two inbred lines (86,101 and 90,196) with significant differences in sulforaphane content, coupled with extensive genotypic and phenotypic data from two independent environments. A linkage map consisting of 438 simple sequence repeats markers was constructed, covering a length of 1168.26 cM. A total of 18 QTLs for sulforaphane metabolism in broccoli florets were detected, 10 were detected in 2017, and the other 8 were detected in 2018. The LOD values of all QTLs ranged from 3.06 to 14.47, explaining 1.74–7.03% of the biochemical variation between two years. Finally, 6 QTLs (qSF-C3-1, qSF-C3-2, qSF-C3-3, qSF-C3-5, qSF-C3-6 and qSF-C7) were stably detected in more than one environment, each accounting for 4.54–7.03% of the phenotypic variation explained (PVE) and a total of 30.88–34.86% of PVE. Our study provides new insights into sulforaphane metabolism in broccoli florets and marker-assisted selection breeding in Brassica oleracea crops.
format	article
author	Zhansheng Li Yumei Liu Suxia Yuan Fengqing Han Zhiyuan Fang Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Yong Wang Jialei Ji
author_facet	Zhansheng Li Yumei Liu Suxia Yuan Fengqing Han Zhiyuan Fang Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Yong Wang Jialei Ji
author_sort	Zhansheng Li
title	Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population
title_short	Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population
title_full	Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population
title_fullStr	Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population
title_full_unstemmed	Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population
title_sort	fine mapping of the major qtls for biochemical variation of sulforaphane in broccoli florets using a dh population
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ec66e8092a7a4f0db1f6ac1cdc488561
work_keys_str_mv	AT zhanshengli finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT yumeiliu finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT suxiayuan finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT fengqinghan finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT zhiyuanfang finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT limeiyang finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT muzhuang finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT yangyongzhang finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT honghaolv finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT yongwang finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation AT jialeiji finemappingofthemajorqtlsforbiochemicalvariationofsulforaphaneinbroccolifloretsusingadhpopulation
_version_	1718379856029286400

Fine mapping of the major QTLs for biochemical variation of sulforaphane in broccoli florets using a DH population

Ejemplares similares