Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.

In actively dividing eukaryotic cells, chromosome ends (telomeres) are subject to progressive shortening, unless they are maintained by the action of telomerase, a dedicated enzyme that adds DNA sequence repeats to chromosomal 3'end. For its enzymatic function on telomeres, telomerase requires...

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Autores principales:	Cornelia Frohnert, Saskia Hutten, Sarah Wälde, Annegret Nath, Ralph H Kehlenbach
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec68d5d4f7d44e4b9a355595c1f88483
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spelling	oai:doaj.org-article:ec68d5d4f7d44e4b9a355595c1f884832021-11-18T08:31:48ZImportin 7 and Nup358 promote nuclear import of the protein component of human telomerase.1932-620310.1371/journal.pone.0088887https://doaj.org/article/ec68d5d4f7d44e4b9a355595c1f884832014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24586428/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In actively dividing eukaryotic cells, chromosome ends (telomeres) are subject to progressive shortening, unless they are maintained by the action of telomerase, a dedicated enzyme that adds DNA sequence repeats to chromosomal 3'end. For its enzymatic function on telomeres, telomerase requires nuclear import of its protein component (hTERT in human cells) and assembly with the RNA component, TERC. We now confirm a major nuclear localization signal (NLS) in the N-terminal region of hTERT and describe a novel one in the C-terminal part. Using an siRNA approach to deplete several import receptors, we identify importin 7 as a soluble nuclear transport factor that is required for efficient import. At the level of the nuclear pore complex (NPC), Nup358, a nucleoporin that forms the cytoplasmic filaments of the NPC, plays an important role in nuclear import of hTERT. A structure-function analysis of Nup358 revealed that the zinc finger region of the nucleoporin is of particular importance for transport of hTERT. Together, our study sheds light on the nuclear import pathway of hTERT.Cornelia FrohnertSaskia HuttenSarah WäldeAnnegret NathRalph H KehlenbachPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88887 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cornelia Frohnert Saskia Hutten Sarah Wälde Annegret Nath Ralph H Kehlenbach Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.
description	In actively dividing eukaryotic cells, chromosome ends (telomeres) are subject to progressive shortening, unless they are maintained by the action of telomerase, a dedicated enzyme that adds DNA sequence repeats to chromosomal 3'end. For its enzymatic function on telomeres, telomerase requires nuclear import of its protein component (hTERT in human cells) and assembly with the RNA component, TERC. We now confirm a major nuclear localization signal (NLS) in the N-terminal region of hTERT and describe a novel one in the C-terminal part. Using an siRNA approach to deplete several import receptors, we identify importin 7 as a soluble nuclear transport factor that is required for efficient import. At the level of the nuclear pore complex (NPC), Nup358, a nucleoporin that forms the cytoplasmic filaments of the NPC, plays an important role in nuclear import of hTERT. A structure-function analysis of Nup358 revealed that the zinc finger region of the nucleoporin is of particular importance for transport of hTERT. Together, our study sheds light on the nuclear import pathway of hTERT.
format	article
author	Cornelia Frohnert Saskia Hutten Sarah Wälde Annegret Nath Ralph H Kehlenbach
author_facet	Cornelia Frohnert Saskia Hutten Sarah Wälde Annegret Nath Ralph H Kehlenbach
author_sort	Cornelia Frohnert
title	Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.
title_short	Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.
title_full	Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.
title_fullStr	Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.
title_full_unstemmed	Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.
title_sort	importin 7 and nup358 promote nuclear import of the protein component of human telomerase.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ec68d5d4f7d44e4b9a355595c1f88483
work_keys_str_mv	AT corneliafrohnert importin7andnup358promotenuclearimportoftheproteincomponentofhumantelomerase AT saskiahutten importin7andnup358promotenuclearimportoftheproteincomponentofhumantelomerase AT sarahwalde importin7andnup358promotenuclearimportoftheproteincomponentofhumantelomerase AT annegretnath importin7andnup358promotenuclearimportoftheproteincomponentofhumantelomerase AT ralphhkehlenbach importin7andnup358promotenuclearimportoftheproteincomponentofhumantelomerase
_version_	1718421716886093824

Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.

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