Molecular Reconstruction of the Diet in Human Stool Samples

ABSTRACT Understanding dietary effects on the gut microbial composition is one of the key questions in human microbiome research. It is highly important to have reliable dietary data on the stool samples to unambiguously link the microbiome composition to food intake. Often, however, self-reported d...

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Autor principal:	Frank Maixner
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	DNA barcodes diet microbiome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ec6d6986da3347d8ab06a8697c3db7e0
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spelling	oai:doaj.org-article:ec6d6986da3347d8ab06a8697c3db7e02021-12-02T18:15:44ZMolecular Reconstruction of the Diet in Human Stool Samples10.1128/mSystems.00634-192379-5077https://doaj.org/article/ec6d6986da3347d8ab06a8697c3db7e02019-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00634-19https://doaj.org/toc/2379-5077ABSTRACT Understanding dietary effects on the gut microbial composition is one of the key questions in human microbiome research. It is highly important to have reliable dietary data on the stool samples to unambiguously link the microbiome composition to food intake. Often, however, self-reported diet surveys have low accuracy and can be misleading. Thereby, additional molecular biology-based methods could help to revise the diet composition. The article by Reese et al. [A. T. Reese, T. R. Kartzinel, B. L. Petrone, P. J. Turnbaugh, et al., mSystems 4(5):e00458-19, 2019, https://doi.org/10.1128/mSystems.00458-19] in a recent issue of mSystems describes a DNA metabarcoding strategy targeting chloroplast DNA markers in stool samples from 11 human subjects consuming both controlled and freely selected diets. The aim of this study was to evaluate the efficiency of this molecular method in detecting plant remains in the sample compared to the written dietary records. This study displays an important first step in implementing molecular dietary reconstructions in stool microbiome studies which will finally help to increase the accuracy of dietary metadata.Frank MaixnerAmerican Society for MicrobiologyarticleDNA barcodesdietmicrobiomeMicrobiologyQR1-502ENmSystems, Vol 4, Iss 6 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	DNA barcodes diet microbiome Microbiology QR1-502
spellingShingle	DNA barcodes diet microbiome Microbiology QR1-502 Frank Maixner Molecular Reconstruction of the Diet in Human Stool Samples
description	ABSTRACT Understanding dietary effects on the gut microbial composition is one of the key questions in human microbiome research. It is highly important to have reliable dietary data on the stool samples to unambiguously link the microbiome composition to food intake. Often, however, self-reported diet surveys have low accuracy and can be misleading. Thereby, additional molecular biology-based methods could help to revise the diet composition. The article by Reese et al. [A. T. Reese, T. R. Kartzinel, B. L. Petrone, P. J. Turnbaugh, et al., mSystems 4(5):e00458-19, 2019, https://doi.org/10.1128/mSystems.00458-19] in a recent issue of mSystems describes a DNA metabarcoding strategy targeting chloroplast DNA markers in stool samples from 11 human subjects consuming both controlled and freely selected diets. The aim of this study was to evaluate the efficiency of this molecular method in detecting plant remains in the sample compared to the written dietary records. This study displays an important first step in implementing molecular dietary reconstructions in stool microbiome studies which will finally help to increase the accuracy of dietary metadata.
format	article
author	Frank Maixner
author_facet	Frank Maixner
author_sort	Frank Maixner
title	Molecular Reconstruction of the Diet in Human Stool Samples
title_short	Molecular Reconstruction of the Diet in Human Stool Samples
title_full	Molecular Reconstruction of the Diet in Human Stool Samples
title_fullStr	Molecular Reconstruction of the Diet in Human Stool Samples
title_full_unstemmed	Molecular Reconstruction of the Diet in Human Stool Samples
title_sort	molecular reconstruction of the diet in human stool samples
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/ec6d6986da3347d8ab06a8697c3db7e0
work_keys_str_mv	AT frankmaixner molecularreconstructionofthedietinhumanstoolsamples
_version_	1718378367870304256

Molecular Reconstruction of the Diet in Human Stool Samples

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