Cytokines and chemokines expression in serum of patients with neuromyelitis optica
Nanping Ai,* Hongjuan Liu,* Huanfen Zhou, Dahe Lin, Junqing Wang, Mo Yang, Honglu Song, Mingming Sun, Quangang Xu, Shihui Wei Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, People’s Republic of China *These authors contributed equally to this work Objective:...
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Dove Medical Press
2019
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oai:doaj.org-article:ec72bcf93cbc4324bc4a6922091a85a42021-12-02T04:47:16ZCytokines and chemokines expression in serum of patients with neuromyelitis optica1178-2021https://doaj.org/article/ec72bcf93cbc4324bc4a6922091a85a42019-01-01T00:00:00Zhttps://www.dovepress.com/cytokines-and-chemokines-expression-in-serum-of-patients-with-neuromye-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Nanping Ai,* Hongjuan Liu,* Huanfen Zhou, Dahe Lin, Junqing Wang, Mo Yang, Honglu Song, Mingming Sun, Quangang Xu, Shihui Wei Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, People’s Republic of China *These authors contributed equally to this work Objective: To study the differences in immunopathogenesis based on chemokine profile in neuromyelitis optica patients positive for AQP4 antibodies or MOG antibodies.Patients and methods: We measured 52 cytokines/chemokines using ELISA in 59 serum samples, which were divided into three groups according to CBA results: HCs (n=16), AQP4+(n=20) and MOG+ (n=23). The regression equation (R2>0.98) of the standard curve was calculated according to the standard concentration and the corresponding A value. And then the corresponding sample concentration was calculated according to the A value of the sample.Results: Eleven of 52 measured serum cytokine/chemokines (CCL22/MDC, CCL13/MCP-4, CCL21/6Ckine, CCL27/CTACK, CCL8/MCP-2, CXCL14/BRAK, Contactin-1, Kallilrein 6/Neurosin, Midkine, VCAM-1 and Fas) were significantly different between MOG+ group and controls. Ten of 52 measured serum cytokine/chemokines (CCL1/I-309, CCL22/MDC, CCL28, CCL17/TARC, CCL27/CTACK, CXCL2/GRO beta, Contactin-1, Midkine, Chemerin and Synuclein-alpha) were significantly different between AQP4+ group and controls. There was no difference between serum AQP4+ and MOG+ groups for CC chemokines. All measured chemokines CXC except CXCL6/GCP-2 showed no significant differences in serum AQP4+ group compared to MOG+ group. However, there was significant difference between serum AQP4+ and MOG+ groups for C5/C5a and Midkine. C5/C5a and Midkine were significantly higher in AQP4+ group compared to MOG+ group (P<0.05). Conclusion: Our findings suggest that the differences of mean concentration in CXCL6/GCP-2, Midkine and C5/C5a probably reveal different immunologic mechanism between AQP4+ NMO and MOG+ NMO. This cytokine/chemokine profiling provides new insight into NMO pathogenesis associated with MOG antibody seropositivity and provides guidance to monitor inflammation and response to treatment in a way. Keywords: NMO, AQP4 antibodies, MOG antibodies, cytokines, chemokinesAi NPLiu HJZhou HFLin DHWang JQYang MSong HGSun MMXu QGWei SHDove Medical PressarticleNMOAQP4 antibodiesMOG antibodiescytokineschemokinesNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 303-310 (2019) |
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NMO AQP4 antibodies MOG antibodies cytokines chemokines Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 |
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NMO AQP4 antibodies MOG antibodies cytokines chemokines Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Ai NP Liu HJ Zhou HF Lin DH Wang JQ Yang M Song HG Sun MM Xu QG Wei SH Cytokines and chemokines expression in serum of patients with neuromyelitis optica |
description |
Nanping Ai,* Hongjuan Liu,* Huanfen Zhou, Dahe Lin, Junqing Wang, Mo Yang, Honglu Song, Mingming Sun, Quangang Xu, Shihui Wei Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, People’s Republic of China *These authors contributed equally to this work Objective: To study the differences in immunopathogenesis based on chemokine profile in neuromyelitis optica patients positive for AQP4 antibodies or MOG antibodies.Patients and methods: We measured 52 cytokines/chemokines using ELISA in 59 serum samples, which were divided into three groups according to CBA results: HCs (n=16), AQP4+(n=20) and MOG+ (n=23). The regression equation (R2>0.98) of the standard curve was calculated according to the standard concentration and the corresponding A value. And then the corresponding sample concentration was calculated according to the A value of the sample.Results: Eleven of 52 measured serum cytokine/chemokines (CCL22/MDC, CCL13/MCP-4, CCL21/6Ckine, CCL27/CTACK, CCL8/MCP-2, CXCL14/BRAK, Contactin-1, Kallilrein 6/Neurosin, Midkine, VCAM-1 and Fas) were significantly different between MOG+ group and controls. Ten of 52 measured serum cytokine/chemokines (CCL1/I-309, CCL22/MDC, CCL28, CCL17/TARC, CCL27/CTACK, CXCL2/GRO beta, Contactin-1, Midkine, Chemerin and Synuclein-alpha) were significantly different between AQP4+ group and controls. There was no difference between serum AQP4+ and MOG+ groups for CC chemokines. All measured chemokines CXC except CXCL6/GCP-2 showed no significant differences in serum AQP4+ group compared to MOG+ group. However, there was significant difference between serum AQP4+ and MOG+ groups for C5/C5a and Midkine. C5/C5a and Midkine were significantly higher in AQP4+ group compared to MOG+ group (P<0.05). Conclusion: Our findings suggest that the differences of mean concentration in CXCL6/GCP-2, Midkine and C5/C5a probably reveal different immunologic mechanism between AQP4+ NMO and MOG+ NMO. This cytokine/chemokine profiling provides new insight into NMO pathogenesis associated with MOG antibody seropositivity and provides guidance to monitor inflammation and response to treatment in a way. Keywords: NMO, AQP4 antibodies, MOG antibodies, cytokines, chemokines |
format |
article |
author |
Ai NP Liu HJ Zhou HF Lin DH Wang JQ Yang M Song HG Sun MM Xu QG Wei SH |
author_facet |
Ai NP Liu HJ Zhou HF Lin DH Wang JQ Yang M Song HG Sun MM Xu QG Wei SH |
author_sort |
Ai NP |
title |
Cytokines and chemokines expression in serum of patients with neuromyelitis optica |
title_short |
Cytokines and chemokines expression in serum of patients with neuromyelitis optica |
title_full |
Cytokines and chemokines expression in serum of patients with neuromyelitis optica |
title_fullStr |
Cytokines and chemokines expression in serum of patients with neuromyelitis optica |
title_full_unstemmed |
Cytokines and chemokines expression in serum of patients with neuromyelitis optica |
title_sort |
cytokines and chemokines expression in serum of patients with neuromyelitis optica |
publisher |
Dove Medical Press |
publishDate |
2019 |
url |
https://doaj.org/article/ec72bcf93cbc4324bc4a6922091a85a4 |
work_keys_str_mv |
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