Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury

Aim: Hyperleptinemia potentiates the effects of many atherogenic factors, such as inflammation, platelet aggregation, migration, hypertrophy, proliferation of vascular smooth muscle cells, and endothelial cell dysfunction. The present study analysed the effects of long-term hyperleptinemia in an in...

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Autores principales:	Ekaterina A. Polyakova, Evgeny N. Mikhaylov, Michael M. Galagudza, Evgeny V. Shlyakhto
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Leptin JAK/STAT pathway Myocardial infarction Hemodynamics Arrhythmias Dyslipidemia Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/ec77f7a4774e4dbdbec59fe4395a8910
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spelling	oai:doaj.org-article:ec77f7a4774e4dbdbec59fe4395a89102021-12-02T05:03:26ZHyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury2405-844010.1016/j.heliyon.2021.e08491https://doaj.org/article/ec77f7a4774e4dbdbec59fe4395a89102021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021025949https://doaj.org/toc/2405-8440Aim: Hyperleptinemia potentiates the effects of many atherogenic factors, such as inflammation, platelet aggregation, migration, hypertrophy, proliferation of vascular smooth muscle cells, and endothelial cell dysfunction. The present study analysed the effects of long-term hyperleptinemia in an in vivo myocardial ischemia-reperfusion model to demonstrate whether the in vivo deleterious effect also affects cardiac structure and function. Main methods: Rats were subcutaneously administered leptin for 8 days to estimate the involvement of the JAK/STAT pathway. Data from 58 male Wistar rats were included in the final analysis. Myocardial infarction (MI) was modelled by the 30-minute ligation of the main left coronary artery followed by 120-minute reperfusion. Hemodynamic measurements, electrocardiography monitoring, echocardiography, myocardial infarct size and area at risk, blood biochemical parameters, leptin, IL-6, TNF-alpha, FGF-21, and cardiomyocyte morphology were measured. The expression of JAK2, p-JAK2, STAT3, p-STAT3 was assessed by Western Blot analysis. Statistical analyses were performed using IBM SPSS Statistics v.26. Key findings: Eight-day hyperleptinemia in rats leads to an increase in blood pressure and heart rate, myocardial hypertrophy, impaired LV function, the frequency of ischemic arrhythmias, dyslipidemia, systemic inflammation, and the size of induced myocardial infarction. Significance: The blockade of the JAK/STAT signalling pathway effectively reverses the negative effects of leptin, including increased blood pressure and total cholesterol.Ekaterina A. PolyakovaEvgeny N. MikhaylovMichael M. GalagudzaEvgeny V. ShlyakhtoElsevierarticleLeptinJAK/STAT pathwayMyocardial infarctionHemodynamicsArrhythmiasDyslipidemiaScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08491- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Leptin JAK/STAT pathway Myocardial infarction Hemodynamics Arrhythmias Dyslipidemia Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Leptin JAK/STAT pathway Myocardial infarction Hemodynamics Arrhythmias Dyslipidemia Science (General) Q1-390 Social sciences (General) H1-99 Ekaterina A. Polyakova Evgeny N. Mikhaylov Michael M. Galagudza Evgeny V. Shlyakhto Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury
description	Aim: Hyperleptinemia potentiates the effects of many atherogenic factors, such as inflammation, platelet aggregation, migration, hypertrophy, proliferation of vascular smooth muscle cells, and endothelial cell dysfunction. The present study analysed the effects of long-term hyperleptinemia in an in vivo myocardial ischemia-reperfusion model to demonstrate whether the in vivo deleterious effect also affects cardiac structure and function. Main methods: Rats were subcutaneously administered leptin for 8 days to estimate the involvement of the JAK/STAT pathway. Data from 58 male Wistar rats were included in the final analysis. Myocardial infarction (MI) was modelled by the 30-minute ligation of the main left coronary artery followed by 120-minute reperfusion. Hemodynamic measurements, electrocardiography monitoring, echocardiography, myocardial infarct size and area at risk, blood biochemical parameters, leptin, IL-6, TNF-alpha, FGF-21, and cardiomyocyte morphology were measured. The expression of JAK2, p-JAK2, STAT3, p-STAT3 was assessed by Western Blot analysis. Statistical analyses were performed using IBM SPSS Statistics v.26. Key findings: Eight-day hyperleptinemia in rats leads to an increase in blood pressure and heart rate, myocardial hypertrophy, impaired LV function, the frequency of ischemic arrhythmias, dyslipidemia, systemic inflammation, and the size of induced myocardial infarction. Significance: The blockade of the JAK/STAT signalling pathway effectively reverses the negative effects of leptin, including increased blood pressure and total cholesterol.
format	article
author	Ekaterina A. Polyakova Evgeny N. Mikhaylov Michael M. Galagudza Evgeny V. Shlyakhto
author_facet	Ekaterina A. Polyakova Evgeny N. Mikhaylov Michael M. Galagudza Evgeny V. Shlyakhto
author_sort	Ekaterina A. Polyakova
title	Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury
title_short	Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury
title_full	Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury
title_fullStr	Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury
title_full_unstemmed	Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury
title_sort	hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ec77f7a4774e4dbdbec59fe4395a8910
work_keys_str_mv	AT ekaterinaapolyakova hyperleptinemiaresultsinsystemicinflammationandtheexacerbationofischemiareperfusionmyocardialinjury AT evgenynmikhaylov hyperleptinemiaresultsinsystemicinflammationandtheexacerbationofischemiareperfusionmyocardialinjury AT michaelmgalagudza hyperleptinemiaresultsinsystemicinflammationandtheexacerbationofischemiareperfusionmyocardialinjury AT evgenyvshlyakhto hyperleptinemiaresultsinsystemicinflammationandtheexacerbationofischemiareperfusionmyocardialinjury
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Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury

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