COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758

Background. Pseudotuberculosis is still relevant problem in medical science and public health of Russia and other countries. Typing of Y. рseudotuberculosis strains by their CRISPR systems is a perspective tool for monitoring of Yersinia populations as was shown in Y. pestis.Aims. Here we describe a...

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Autores principales:	N. P. Peretolchina, A. Y. Borisenko, Yu. P. Dzhioev, V. I. Zlobin
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2018
Materias:	crispr-cas система y. pseudotuberculosis Science Q
Acceso en línea:	https://doaj.org/article/ec7eb327acb34912ac6c1da47c44faaa
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spelling	oai:doaj.org-article:ec7eb327acb34912ac6c1da47c44faaa2021-11-23T06:14:41ZCOMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP317582541-94202587-959610.29413/ABS.2018-3.5.8https://doaj.org/article/ec7eb327acb34912ac6c1da47c44faaa2018-09-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/707https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Pseudotuberculosis is still relevant problem in medical science and public health of Russia and other countries. Typing of Y. рseudotuberculosis strains by their CRISPR systems is a perspective tool for monitoring of Yersinia populations as was shown in Y. pestis.Aims. Here we describe and compare CRISPR-Cas systems of Yersinia pseudotuberculosis strains IP32953 and IP31758 causing classic pseudotuberculosis and Far-East scarlet-like fever (FESLF) respectively.Materials and methods. Complete genomes of Y. pseudotuberculosis IP329353 and IP31758 (NC_006155 and NC_009708 respectively) were obtained from NCBI Nucleotide Database. Search; identification; and analysis of CRISPR systems were carried out by online-tools CRISPROne; CRISPRDetect; and CRISPRTarget.Results and discussion. Analyzed strains have CRISPR-Cas systems that include one set of cas-genes and arrays situated at the long distances from each other. We defined three CRISPR arrays in Y. pseudotuberculosis IP32953 by the combination of program methods. CRISPR-Cas system of this strain consist of array YP1 located near cas-genes; arrays YP2 and YP3. CRISPR-Cas system of Y. pseudotuberculosis IP31758 includes two arrays – YP1 and YP3. CRISPR systems do not share similar spacers. CRISPR systems of the analyzed strains differ in CRISPR loci and cas-protein structures that can be used as specific marks of analyzed strains.Conclusions. We suggest that acquisition of certain spacers may play a role in evolution and divergence of Y. pseudotuberculosis strains.N. P. PeretolchinaA. Y. BorisenkoYu. P. DzhioevV. I. ZlobinScientific Сentre for Family Health and Human Reproduction Problemsarticlecrispr-cas системаy. pseudotuberculosisScienceQRUActa Biomedica Scientifica, Vol 3, Iss 5, Pp 54-59 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	crispr-cas система y. pseudotuberculosis Science Q
spellingShingle	crispr-cas система y. pseudotuberculosis Science Q N. P. Peretolchina A. Y. Borisenko Yu. P. Dzhioev V. I. Zlobin COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758
description	Background. Pseudotuberculosis is still relevant problem in medical science and public health of Russia and other countries. Typing of Y. рseudotuberculosis strains by their CRISPR systems is a perspective tool for monitoring of Yersinia populations as was shown in Y. pestis.Aims. Here we describe and compare CRISPR-Cas systems of Yersinia pseudotuberculosis strains IP32953 and IP31758 causing classic pseudotuberculosis and Far-East scarlet-like fever (FESLF) respectively.Materials and methods. Complete genomes of Y. pseudotuberculosis IP329353 and IP31758 (NC_006155 and NC_009708 respectively) were obtained from NCBI Nucleotide Database. Search; identification; and analysis of CRISPR systems were carried out by online-tools CRISPROne; CRISPRDetect; and CRISPRTarget.Results and discussion. Analyzed strains have CRISPR-Cas systems that include one set of cas-genes and arrays situated at the long distances from each other. We defined three CRISPR arrays in Y. pseudotuberculosis IP32953 by the combination of program methods. CRISPR-Cas system of this strain consist of array YP1 located near cas-genes; arrays YP2 and YP3. CRISPR-Cas system of Y. pseudotuberculosis IP31758 includes two arrays – YP1 and YP3. CRISPR systems do not share similar spacers. CRISPR systems of the analyzed strains differ in CRISPR loci and cas-protein structures that can be used as specific marks of analyzed strains.Conclusions. We suggest that acquisition of certain spacers may play a role in evolution and divergence of Y. pseudotuberculosis strains.
format	article
author	N. P. Peretolchina A. Y. Borisenko Yu. P. Dzhioev V. I. Zlobin
author_facet	N. P. Peretolchina A. Y. Borisenko Yu. P. Dzhioev V. I. Zlobin
author_sort	N. P. Peretolchina
title	COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758
title_short	COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758
title_full	COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758
title_fullStr	COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758
title_full_unstemmed	COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758
title_sort	comparative analysis of crispr-cas system structures of yersinia pseudotuberculosis ip32953 and ip31758
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2018
url	https://doaj.org/article/ec7eb327acb34912ac6c1da47c44faaa
work_keys_str_mv	AT npperetolchina comparativeanalysisofcrisprcassystemstructuresofyersiniapseudotuberculosisip32953andip31758 AT ayborisenko comparativeanalysisofcrisprcassystemstructuresofyersiniapseudotuberculosisip32953andip31758 AT yupdzhioev comparativeanalysisofcrisprcassystemstructuresofyersiniapseudotuberculosisip32953andip31758 AT vizlobin comparativeanalysisofcrisprcassystemstructuresofyersiniapseudotuberculosisip32953andip31758
_version_	1718416982775169024

COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758

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