Proper microtubule structure is vital for timely progression through meiosis in fission yeast.

Cells of the fission yeast Schizosaccharomyces pombe normally reproduce by mitotic division in the haploid state. When subjected to nutrient starvation, two haploid cells fuse and undergo karyogamy, forming a diploid cell that initiates meiosis to form four haploid spores. Here, we show that deletio...

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Autores principales:	Akira Yamashita, Yoshihiro Fujita, Masayuki Yamamoto
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec856f42c6024ee68106d4e7dcaf6db7
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spelling	oai:doaj.org-article:ec856f42c6024ee68106d4e7dcaf6db72021-11-18T07:42:58ZProper microtubule structure is vital for timely progression through meiosis in fission yeast.1932-620310.1371/journal.pone.0065082https://doaj.org/article/ec856f42c6024ee68106d4e7dcaf6db72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23755176/?tool=EBIhttps://doaj.org/toc/1932-6203Cells of the fission yeast Schizosaccharomyces pombe normally reproduce by mitotic division in the haploid state. When subjected to nutrient starvation, two haploid cells fuse and undergo karyogamy, forming a diploid cell that initiates meiosis to form four haploid spores. Here, we show that deletion of the mal3 gene, which encodes a homolog of microtubule regulator EB1, produces aberrant asci carrying more than four spores. The mal3 deletion mutant cells have a disordered cytoplasmic microtubule structure during karyogamy and initiate meiosis before completion of karyogamy, resulting in twin haploid meiosis in the zygote. Treatment with anti-microtubule drugs mimics this phenotype. Mutants defective in karyogamy or mutants prone to initiate haploid meiosis exaggerate the phenotype of the mal3 deletion mutant. Our results indicate that proper microtubule structure is required for ordered progression through the meiotic cycle. Furthermore, the results of our study suggest that fission yeast do not monitor ploidy during meiosis.Akira YamashitaYoshihiro FujitaMasayuki YamamotoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65082 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akira Yamashita Yoshihiro Fujita Masayuki Yamamoto Proper microtubule structure is vital for timely progression through meiosis in fission yeast.
description	Cells of the fission yeast Schizosaccharomyces pombe normally reproduce by mitotic division in the haploid state. When subjected to nutrient starvation, two haploid cells fuse and undergo karyogamy, forming a diploid cell that initiates meiosis to form four haploid spores. Here, we show that deletion of the mal3 gene, which encodes a homolog of microtubule regulator EB1, produces aberrant asci carrying more than four spores. The mal3 deletion mutant cells have a disordered cytoplasmic microtubule structure during karyogamy and initiate meiosis before completion of karyogamy, resulting in twin haploid meiosis in the zygote. Treatment with anti-microtubule drugs mimics this phenotype. Mutants defective in karyogamy or mutants prone to initiate haploid meiosis exaggerate the phenotype of the mal3 deletion mutant. Our results indicate that proper microtubule structure is required for ordered progression through the meiotic cycle. Furthermore, the results of our study suggest that fission yeast do not monitor ploidy during meiosis.
format	article
author	Akira Yamashita Yoshihiro Fujita Masayuki Yamamoto
author_facet	Akira Yamashita Yoshihiro Fujita Masayuki Yamamoto
author_sort	Akira Yamashita
title	Proper microtubule structure is vital for timely progression through meiosis in fission yeast.
title_short	Proper microtubule structure is vital for timely progression through meiosis in fission yeast.
title_full	Proper microtubule structure is vital for timely progression through meiosis in fission yeast.
title_fullStr	Proper microtubule structure is vital for timely progression through meiosis in fission yeast.
title_full_unstemmed	Proper microtubule structure is vital for timely progression through meiosis in fission yeast.
title_sort	proper microtubule structure is vital for timely progression through meiosis in fission yeast.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ec856f42c6024ee68106d4e7dcaf6db7
work_keys_str_mv	AT akirayamashita propermicrotubulestructureisvitalfortimelyprogressionthroughmeiosisinfissionyeast AT yoshihirofujita propermicrotubulestructureisvitalfortimelyprogressionthroughmeiosisinfissionyeast AT masayukiyamamoto propermicrotubulestructureisvitalfortimelyprogressionthroughmeiosisinfissionyeast
_version_	1718423071655723008

Proper microtubule structure is vital for timely progression through meiosis in fission yeast.

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