Preparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with H9c2 cells.

This study deals with the preparation and characterization of selenium incorporated guar gum nanoparticle (SGG), and its effect on H9c2 cardiomyoblast. Herein, nanoprecipitation techniques had been employed for the preparation of SGG nanoparticle. The prepared nanoparticle had been subjected to vari...

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Autores principales:	Rema Sreenivasan Soumya, Vadavanath Prabhakaran Vineetha, Premachandran Latha Reshma, Kozhiparambil Gopalan Raghu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec8e8c823db7410aac8591672ea2aecc
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spelling	oai:doaj.org-article:ec8e8c823db7410aac8591672ea2aecc2021-11-18T08:53:06ZPreparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with H9c2 cells.1932-620310.1371/journal.pone.0074411https://doaj.org/article/ec8e8c823db7410aac8591672ea2aecc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24098647/?tool=EBIhttps://doaj.org/toc/1932-6203This study deals with the preparation and characterization of selenium incorporated guar gum nanoparticle (SGG), and its effect on H9c2 cardiomyoblast. Herein, nanoprecipitation techniques had been employed for the preparation of SGG nanoparticle. The prepared nanoparticle had been subjected to various types of analytical techniques like transmission electron microscopy (TEM), X-ray diffraction (XRD) and particle size analysis to confirm the characteristics of nanoparticle as well as for selenium incorporation. Physical characterization of nanoparticle showed that the size of nanoparticles increase upto ∼69-173 nm upon selenium incorporation from ∼41-132 nm. Then the prepared nanoparticles were evaluated for its effect on H9c2 cells. In this regard, the effect of nanoparticle on various vital parameters of H9c2 cells was studied. Parameters like cell viability, uptake of selenium incorporated guar gum nanoparticle by the cells, effect of SGG on DNA integrity, apoptosis, reactive oxygen species generation, alteration in transmembrane potential of mitochondria and cytoskeletal integrity had been investigated. Viability results showed that up to 25 nM of SGG was safe (10.31%) but beyond that it induces cytotoxicity. Cellular uptake of selenium showed that cell permeability for SGG is significantly high compared to normal selenium (7.2 nM of selenium for 25 nM SGG compared with 5.2 nM selenium for 25 nM sodium selenite). There was no apoptosis with SGG and also it protects DNA from hydroxyl radical induced breakage. Likewise no adverse effect on mitochondria and cytoskeleton was observed for 25 nM of SGG. Overall results reveal that SGG is highly suitable for biomedical research application.Rema Sreenivasan SoumyaVadavanath Prabhakaran VineethaPremachandran Latha ReshmaKozhiparambil Gopalan RaghuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74411 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rema Sreenivasan Soumya Vadavanath Prabhakaran Vineetha Premachandran Latha Reshma Kozhiparambil Gopalan Raghu Preparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with H9c2 cells.
description	This study deals with the preparation and characterization of selenium incorporated guar gum nanoparticle (SGG), and its effect on H9c2 cardiomyoblast. Herein, nanoprecipitation techniques had been employed for the preparation of SGG nanoparticle. The prepared nanoparticle had been subjected to various types of analytical techniques like transmission electron microscopy (TEM), X-ray diffraction (XRD) and particle size analysis to confirm the characteristics of nanoparticle as well as for selenium incorporation. Physical characterization of nanoparticle showed that the size of nanoparticles increase upto ∼69-173 nm upon selenium incorporation from ∼41-132 nm. Then the prepared nanoparticles were evaluated for its effect on H9c2 cells. In this regard, the effect of nanoparticle on various vital parameters of H9c2 cells was studied. Parameters like cell viability, uptake of selenium incorporated guar gum nanoparticle by the cells, effect of SGG on DNA integrity, apoptosis, reactive oxygen species generation, alteration in transmembrane potential of mitochondria and cytoskeletal integrity had been investigated. Viability results showed that up to 25 nM of SGG was safe (10.31%) but beyond that it induces cytotoxicity. Cellular uptake of selenium showed that cell permeability for SGG is significantly high compared to normal selenium (7.2 nM of selenium for 25 nM SGG compared with 5.2 nM selenium for 25 nM sodium selenite). There was no apoptosis with SGG and also it protects DNA from hydroxyl radical induced breakage. Likewise no adverse effect on mitochondria and cytoskeleton was observed for 25 nM of SGG. Overall results reveal that SGG is highly suitable for biomedical research application.
format	article
author	Rema Sreenivasan Soumya Vadavanath Prabhakaran Vineetha Premachandran Latha Reshma Kozhiparambil Gopalan Raghu
author_facet	Rema Sreenivasan Soumya Vadavanath Prabhakaran Vineetha Premachandran Latha Reshma Kozhiparambil Gopalan Raghu
author_sort	Rema Sreenivasan Soumya
title	Preparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with H9c2 cells.
title_short	Preparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with H9c2 cells.
title_full	Preparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with H9c2 cells.
title_fullStr	Preparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with H9c2 cells.
title_full_unstemmed	Preparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with H9c2 cells.
title_sort	preparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with h9c2 cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ec8e8c823db7410aac8591672ea2aecc
work_keys_str_mv	AT remasreenivasansoumya preparationandcharacterizationofseleniumincorporatedguargumnanoparticleanditsinteractionwithh9c2cells AT vadavanathprabhakaranvineetha preparationandcharacterizationofseleniumincorporatedguargumnanoparticleanditsinteractionwithh9c2cells AT premachandranlathareshma preparationandcharacterizationofseleniumincorporatedguargumnanoparticleanditsinteractionwithh9c2cells AT kozhiparambilgopalanraghu preparationandcharacterizationofseleniumincorporatedguargumnanoparticleanditsinteractionwithh9c2cells
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Preparation and characterization of selenium incorporated guar gum nanoparticle and its interaction with H9c2 cells.

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