Designed synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides

Abstract Highly efficient enrichment of glycopeptides or phosphopeptides from complex biological samples is indispensable for high-throughput mass spectrometry analysis. In this study, for the first time, a “one for two” hydrophilic magnetic amino-functionalized metal-organic framework (MOF) was des...

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Autores principales:	Yiqin Xie, Chunhui Deng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec902b62185443a2830082d331cbe4e8
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spelling	oai:doaj.org-article:ec902b62185443a2830082d331cbe4e82021-12-02T16:06:27ZDesigned synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides10.1038/s41598-017-01341-y2045-2322https://doaj.org/article/ec902b62185443a2830082d331cbe4e82017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01341-yhttps://doaj.org/toc/2045-2322Abstract Highly efficient enrichment of glycopeptides or phosphopeptides from complex biological samples is indispensable for high-throughput mass spectrometry analysis. In this study, for the first time, a “one for two” hydrophilic magnetic amino-functionalized metal-organic framework (MOF) was designed and synthesized for selective enrichment of both glycopeptides and phosphopeptides. A well-known solvo-thermal reaction was adopted to prepare a magnetic core Fe3O4, followed by self- polymerization of dopamine, creating a polydopamine (PDA) onto Fe3O4. Thanks to the hydroxyl and amino group of PDA, Zr3+ was easily adhered to the surface, inducing the following one-pot MOF reaction with amino ligand. After characterization of the as-prepared MOFs (denoted as Fe3O4@PDA@UiO-66-NH2), its ultrahigh surface area, excellent hydrophilicity and strong magnetic responsiveness were highly confirmed. Based on hydrophilic interaction, it was applied to glycopeptide enrichment, while based on strong binding between Zr and phosphopeptides, it was applied to phosphopeptide enrichment, both exhibiting excellent performance in standard proteins and human serum with high sensitivity and selectivity. These results showed the as-prepared MOFs had great potential in proteomics research.Yiqin XieChunhui DengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yiqin Xie Chunhui Deng Designed synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides
description	Abstract Highly efficient enrichment of glycopeptides or phosphopeptides from complex biological samples is indispensable for high-throughput mass spectrometry analysis. In this study, for the first time, a “one for two” hydrophilic magnetic amino-functionalized metal-organic framework (MOF) was designed and synthesized for selective enrichment of both glycopeptides and phosphopeptides. A well-known solvo-thermal reaction was adopted to prepare a magnetic core Fe3O4, followed by self- polymerization of dopamine, creating a polydopamine (PDA) onto Fe3O4. Thanks to the hydroxyl and amino group of PDA, Zr3+ was easily adhered to the surface, inducing the following one-pot MOF reaction with amino ligand. After characterization of the as-prepared MOFs (denoted as Fe3O4@PDA@UiO-66-NH2), its ultrahigh surface area, excellent hydrophilicity and strong magnetic responsiveness were highly confirmed. Based on hydrophilic interaction, it was applied to glycopeptide enrichment, while based on strong binding between Zr and phosphopeptides, it was applied to phosphopeptide enrichment, both exhibiting excellent performance in standard proteins and human serum with high sensitivity and selectivity. These results showed the as-prepared MOFs had great potential in proteomics research.
format	article
author	Yiqin Xie Chunhui Deng
author_facet	Yiqin Xie Chunhui Deng
author_sort	Yiqin Xie
title	Designed synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides
title_short	Designed synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides
title_full	Designed synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides
title_fullStr	Designed synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides
title_full_unstemmed	Designed synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides
title_sort	designed synthesis of a “one for two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ec902b62185443a2830082d331cbe4e8
work_keys_str_mv	AT yiqinxie designedsynthesisofaonefortwohydrophilicmagneticaminofunctionalizedmetalorganicframeworkforhighlyefficientenrichmentofglycopeptidesandphosphopeptides AT chunhuideng designedsynthesisofaonefortwohydrophilicmagneticaminofunctionalizedmetalorganicframeworkforhighlyefficientenrichmentofglycopeptidesandphosphopeptides
_version_	1718384998784958464

Designed synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides

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