Extensive expression differences along porcine small intestine evidenced by transcriptome sequencing.
The aim of this study was to analyse gene expression along the small intestine (duodenum, jejunum, ileum) and in the ileal Peyer's patches in four young pigs with no clinical signs of disease by transcriptome sequencing. Multidimensional scaling evidenced that samples clustered by tissue type r...
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2014
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oai:doaj.org-article:ec968a92053345e0b81f7d4406677e862021-11-18T08:32:49ZExtensive expression differences along porcine small intestine evidenced by transcriptome sequencing.1932-620310.1371/journal.pone.0088515https://doaj.org/article/ec968a92053345e0b81f7d4406677e862014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24533095/?tool=EBIhttps://doaj.org/toc/1932-6203The aim of this study was to analyse gene expression along the small intestine (duodenum, jejunum, ileum) and in the ileal Peyer's patches in four young pigs with no clinical signs of disease by transcriptome sequencing. Multidimensional scaling evidenced that samples clustered by tissue type rather than by individual, thus prefiguring a relevant scenario to draw tissue-specific gene expression profiles. Accordingly, 1,349 genes were found differentially expressed between duodenum and jejunum, and up to 3,455 genes between duodenum and ileum. Additionally, a considerable number of differentially expressed genes were found by comparing duodenum (7,027 genes), jejunum (6,122 genes), and ileum (6,991 genes) with ileal Peyer's patches tissue. Functional analyses revealed that most of the significant differentially expressed genes along small intestinal tissues were involved in the regulation of general biological processes such as cell development, signalling, growth and proliferation, death and survival or cell function and maintenance. These results suggest that the intrinsic large turnover of intestinal tissues would have local specificities at duodenum, ileum and jejunum. In addition, in concordance with their biological function, enteric innate immune pathways were overrepresented in ileal Peyer's patches. The reported data provide an expression map of the cell pathway variation in the different small intestinal tissues. Furthermore, expression levels measured in healthy individuals could help to understand changes in gene expression that occur in dysbiosis or pathological states.Núria MachMustapha BerriDiane EsquerréClaire ChevaleyreGaëtan LemonnierYvon BillonPatricia LepageIsabelle P OswaldJoël DoréClaire Rogel-GaillardJordi EstelléPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88515 (2014) |
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Medicine R Science Q |
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Medicine R Science Q Núria Mach Mustapha Berri Diane Esquerré Claire Chevaleyre Gaëtan Lemonnier Yvon Billon Patricia Lepage Isabelle P Oswald Joël Doré Claire Rogel-Gaillard Jordi Estellé Extensive expression differences along porcine small intestine evidenced by transcriptome sequencing. |
| description |
The aim of this study was to analyse gene expression along the small intestine (duodenum, jejunum, ileum) and in the ileal Peyer's patches in four young pigs with no clinical signs of disease by transcriptome sequencing. Multidimensional scaling evidenced that samples clustered by tissue type rather than by individual, thus prefiguring a relevant scenario to draw tissue-specific gene expression profiles. Accordingly, 1,349 genes were found differentially expressed between duodenum and jejunum, and up to 3,455 genes between duodenum and ileum. Additionally, a considerable number of differentially expressed genes were found by comparing duodenum (7,027 genes), jejunum (6,122 genes), and ileum (6,991 genes) with ileal Peyer's patches tissue. Functional analyses revealed that most of the significant differentially expressed genes along small intestinal tissues were involved in the regulation of general biological processes such as cell development, signalling, growth and proliferation, death and survival or cell function and maintenance. These results suggest that the intrinsic large turnover of intestinal tissues would have local specificities at duodenum, ileum and jejunum. In addition, in concordance with their biological function, enteric innate immune pathways were overrepresented in ileal Peyer's patches. The reported data provide an expression map of the cell pathway variation in the different small intestinal tissues. Furthermore, expression levels measured in healthy individuals could help to understand changes in gene expression that occur in dysbiosis or pathological states. |
| format |
article |
| author |
Núria Mach Mustapha Berri Diane Esquerré Claire Chevaleyre Gaëtan Lemonnier Yvon Billon Patricia Lepage Isabelle P Oswald Joël Doré Claire Rogel-Gaillard Jordi Estellé |
| author_facet |
Núria Mach Mustapha Berri Diane Esquerré Claire Chevaleyre Gaëtan Lemonnier Yvon Billon Patricia Lepage Isabelle P Oswald Joël Doré Claire Rogel-Gaillard Jordi Estellé |
| author_sort |
Núria Mach |
| title |
Extensive expression differences along porcine small intestine evidenced by transcriptome sequencing. |
| title_short |
Extensive expression differences along porcine small intestine evidenced by transcriptome sequencing. |
| title_full |
Extensive expression differences along porcine small intestine evidenced by transcriptome sequencing. |
| title_fullStr |
Extensive expression differences along porcine small intestine evidenced by transcriptome sequencing. |
| title_full_unstemmed |
Extensive expression differences along porcine small intestine evidenced by transcriptome sequencing. |
| title_sort |
extensive expression differences along porcine small intestine evidenced by transcriptome sequencing. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/ec968a92053345e0b81f7d4406677e86 |
| work_keys_str_mv |
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