In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis

Abstract Autologous blood-derived products such as platelet-rich plasma (PRP) are widely used to treat musculoskeletal conditions, including knee osteoarthritis (OA). However, the clinical outcomes after PRP administration are often variable, and there is limited information about the specific chara...

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Autores principales:	Habib Zahir, Bijan Dehghani, Xiaoning Yuan, Yurii Chinenov, Christine Kim, Alissa Burge, Reyna Bandhari, Daniel Nemirov, Patrick Fava, Peter Moley, Hollis Potter, Joseph Nguyen, Brian Halpern, Laura Donlin, Lionel Ivashkiv, Scott Rodeo, Miguel Otero
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ec9a0d1e067948cf827bca6f73851836
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spelling	oai:doaj.org-article:ec9a0d1e067948cf827bca6f738518362021-12-02T15:02:48ZIn vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis10.1038/s41598-021-90174-x2045-2322https://doaj.org/article/ec9a0d1e067948cf827bca6f738518362021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90174-xhttps://doaj.org/toc/2045-2322Abstract Autologous blood-derived products such as platelet-rich plasma (PRP) are widely used to treat musculoskeletal conditions, including knee osteoarthritis (OA). However, the clinical outcomes after PRP administration are often variable, and there is limited information about the specific characteristics of PRP that impact bioactivity and clinical responses. In this study, we aimed to develop an integrative workflow to evaluate responses to PRP in vitro, and to assess if the in vitro responses to PRP are associated with the PRP composition and clinical outcomes in patients with knee OA. To do this, we used a coculture system of macrophages and fibroblasts paired with transcriptomic analyses to comprehensively characterize the modulation of inflammatory responses by PRP in vitro. Relying on patient-reported outcomes and achievement of minimal clinically important differences in OA patients receiving PRP injections, we identified responders and non-responders to the treatment. Comparisons of PRP from these patient groups allowed us to identify differences in the composition and in vitro activity of PRP. We believe that our integrative workflow may enable the development of targeted approaches that rely on PRP and other orthobiologics to treat musculoskeletal pathologies.Habib ZahirBijan DehghaniXiaoning YuanYurii ChinenovChristine KimAlissa BurgeReyna BandhariDaniel NemirovPatrick FavaPeter MoleyHollis PotterJoseph NguyenBrian HalpernLaura DonlinLionel IvashkivScott RodeoMiguel OteroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Habib Zahir Bijan Dehghani Xiaoning Yuan Yurii Chinenov Christine Kim Alissa Burge Reyna Bandhari Daniel Nemirov Patrick Fava Peter Moley Hollis Potter Joseph Nguyen Brian Halpern Laura Donlin Lionel Ivashkiv Scott Rodeo Miguel Otero In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis
description	Abstract Autologous blood-derived products such as platelet-rich plasma (PRP) are widely used to treat musculoskeletal conditions, including knee osteoarthritis (OA). However, the clinical outcomes after PRP administration are often variable, and there is limited information about the specific characteristics of PRP that impact bioactivity and clinical responses. In this study, we aimed to develop an integrative workflow to evaluate responses to PRP in vitro, and to assess if the in vitro responses to PRP are associated with the PRP composition and clinical outcomes in patients with knee OA. To do this, we used a coculture system of macrophages and fibroblasts paired with transcriptomic analyses to comprehensively characterize the modulation of inflammatory responses by PRP in vitro. Relying on patient-reported outcomes and achievement of minimal clinically important differences in OA patients receiving PRP injections, we identified responders and non-responders to the treatment. Comparisons of PRP from these patient groups allowed us to identify differences in the composition and in vitro activity of PRP. We believe that our integrative workflow may enable the development of targeted approaches that rely on PRP and other orthobiologics to treat musculoskeletal pathologies.
format	article
author	Habib Zahir Bijan Dehghani Xiaoning Yuan Yurii Chinenov Christine Kim Alissa Burge Reyna Bandhari Daniel Nemirov Patrick Fava Peter Moley Hollis Potter Joseph Nguyen Brian Halpern Laura Donlin Lionel Ivashkiv Scott Rodeo Miguel Otero
author_facet	Habib Zahir Bijan Dehghani Xiaoning Yuan Yurii Chinenov Christine Kim Alissa Burge Reyna Bandhari Daniel Nemirov Patrick Fava Peter Moley Hollis Potter Joseph Nguyen Brian Halpern Laura Donlin Lionel Ivashkiv Scott Rodeo Miguel Otero
author_sort	Habib Zahir
title	In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis
title_short	In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis
title_full	In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis
title_fullStr	In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis
title_full_unstemmed	In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis
title_sort	in vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ec9a0d1e067948cf827bca6f73851836
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In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis

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