GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer

No specific markers have been identified to detect non-small cell lung cancer (NSCLC) cell-derived exosomes circulating in the blood. Here, we report a new biomarker that distinguishes between cancer and non-cancer cell-derived exosomes. Exosomes isolated from patient plasmas at various pathological...

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Autores principales:	Hyesun Jeong, Byeong Hyeon Choi, JinA Park, Jik-Han Jung, Hyunku Shin, Ka-Won Kang, Yu Hua Quan, Jewon Yu, Ji-Ho Park, Yong Park, Yeonho Choi, Hyun Koo Kim, Sunghoi Hong
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	exosomes non-small cell lung cancer GCC2 biomarkers early detection liquid biopsy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/ec9c5250a33a43519fff7380f8888040
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spelling	oai:doaj.org-article:ec9c5250a33a43519fff7380f88880402021-11-11T15:33:40ZGCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer10.3390/cancers132154822072-6694https://doaj.org/article/ec9c5250a33a43519fff7380f88880402021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5482https://doaj.org/toc/2072-6694No specific markers have been identified to detect non-small cell lung cancer (NSCLC) cell-derived exosomes circulating in the blood. Here, we report a new biomarker that distinguishes between cancer and non-cancer cell-derived exosomes. Exosomes isolated from patient plasmas at various pathological stages of NSCLC, NSCLC cell lines, and human pulmonary alveolar epithelial cells isolated using size exclusion chromatography were characterized. The GRIP and coiled-coil domain-containing 2 (GCC2) protein, involved in endosome-to-Golgi transport, was identified by proteomics analysis of NSCLC cell line-derived exosomes. GCC2 protein levels in the exosomes derived from early-stage NSCLC patients were higher than those from healthy controls. Receiver operating characteristic curve analysis revealed the diagnostic sensitivity and specificity of exosomal GCC2 to be 90% and 75%, respectively. A high area under the curve, 0.844, confirmed that GCC2 levels could effectively distinguish between the exosomes. These results demonstrate GCC2 as a promising early diagnostic biomarker for NSCLC.Hyesun JeongByeong Hyeon ChoiJinA ParkJik-Han JungHyunku ShinKa-Won KangYu Hua QuanJewon YuJi-Ho ParkYong ParkYeonho ChoiHyun Koo KimSunghoi HongMDPI AGarticleexosomesnon-small cell lung cancerGCC2biomarkersearly detectionliquid biopsyNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5482, p 5482 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	exosomes non-small cell lung cancer GCC2 biomarkers early detection liquid biopsy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	exosomes non-small cell lung cancer GCC2 biomarkers early detection liquid biopsy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Hyesun Jeong Byeong Hyeon Choi JinA Park Jik-Han Jung Hyunku Shin Ka-Won Kang Yu Hua Quan Jewon Yu Ji-Ho Park Yong Park Yeonho Choi Hyun Koo Kim Sunghoi Hong GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer
description	No specific markers have been identified to detect non-small cell lung cancer (NSCLC) cell-derived exosomes circulating in the blood. Here, we report a new biomarker that distinguishes between cancer and non-cancer cell-derived exosomes. Exosomes isolated from patient plasmas at various pathological stages of NSCLC, NSCLC cell lines, and human pulmonary alveolar epithelial cells isolated using size exclusion chromatography were characterized. The GRIP and coiled-coil domain-containing 2 (GCC2) protein, involved in endosome-to-Golgi transport, was identified by proteomics analysis of NSCLC cell line-derived exosomes. GCC2 protein levels in the exosomes derived from early-stage NSCLC patients were higher than those from healthy controls. Receiver operating characteristic curve analysis revealed the diagnostic sensitivity and specificity of exosomal GCC2 to be 90% and 75%, respectively. A high area under the curve, 0.844, confirmed that GCC2 levels could effectively distinguish between the exosomes. These results demonstrate GCC2 as a promising early diagnostic biomarker for NSCLC.
format	article
author	Hyesun Jeong Byeong Hyeon Choi JinA Park Jik-Han Jung Hyunku Shin Ka-Won Kang Yu Hua Quan Jewon Yu Ji-Ho Park Yong Park Yeonho Choi Hyun Koo Kim Sunghoi Hong
author_facet	Hyesun Jeong Byeong Hyeon Choi JinA Park Jik-Han Jung Hyunku Shin Ka-Won Kang Yu Hua Quan Jewon Yu Ji-Ho Park Yong Park Yeonho Choi Hyun Koo Kim Sunghoi Hong
author_sort	Hyesun Jeong
title	GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer
title_short	GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer
title_full	GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer
title_fullStr	GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer
title_full_unstemmed	GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer
title_sort	gcc2 as a new early diagnostic biomarker for non-small cell lung cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ec9c5250a33a43519fff7380f8888040
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GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer

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