Transcriptome Profiling Analysis of Bovine Vaginal Epithelial Cell Response to an Isolated <italic toggle="yes">Lactobacillus</italic> Strain

ABSTRACT Lactobacillus strain SQ0048 isolated from bovine vagina has been shown to exhibit specific adherence to the epithelium and to produce inhibitory substances; however, the underlying mechanisms remain unclear. We cultured and identified primary bovine vaginal epithelial cells treated with SQ0...

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Autores principales:	Chunyu Niu, Chao Cheng, Yanmin Liu, Shaolei Huang, Yanru Fu, Peifeng Li
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	primary vaginal epithelial cell culture Lactobacillus transcriptome profiling gene expression RT-qPCR Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ec9e73ff35f64ba696bf4599cc295b7a
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spelling	oai:doaj.org-article:ec9e73ff35f64ba696bf4599cc295b7a2021-12-02T19:46:17ZTranscriptome Profiling Analysis of Bovine Vaginal Epithelial Cell Response to an Isolated <italic toggle="yes">Lactobacillus</italic> Strain10.1128/mSystems.00268-192379-5077https://doaj.org/article/ec9e73ff35f64ba696bf4599cc295b7a2019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00268-19https://doaj.org/toc/2379-5077ABSTRACT Lactobacillus strain SQ0048 isolated from bovine vagina has been shown to exhibit specific adherence to the epithelium and to produce inhibitory substances; however, the underlying mechanisms remain unclear. We cultured and identified primary bovine vaginal epithelial cells treated with SQ0048 to investigate the pathways involved in host cell responses using transcriptome sequencing (RNA-seq). Transcription profiling showed 296 significantly altered differentially expressed genes (DEGs), of which 170 were upregulated and 126 downregulated. Gene Ontology (GO) enrichment analysis of the DEGs revealed significant enrichment of 424 GO terms throughout the differentiation process (P < 0.05). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the DEGs were successfully annotated as members of 171 pathways, with 23 significantly enriched KEGG pathways (P < 0.05). A relatively high number of genes were enriched for the endoplasmic reticulum protein processing and interleukin-17 (IL-17) signaling pathways and for antigen processing and presentation. DEGs were verified by quantitative reverse transcription-PCR (RT-qPCR) and determination of which were most enriched for endoplasmic reticulum protein processing pathways, the activation of which might be a major factor underlying Lactobacillus adhesion to cells and pathogenic inhibition. IMPORTANCE Bovine bacterial vaginitis causes infertility, abortion, and postpartum uterine diseases, causing serious economic loss to the dairy industry. The large-scale use of antibiotics destroys normal genital tract flora and hinders the defense mechanisms of the host. Recent research suggests that lactobacilli present in the vaginal microflora of healthy cows constitute the primary microbiological barrier to infection by genital pathogens, exerting a protective role on the reproductive tract via specific adherence to the epithelium and the production of inhibitory substances. Our research identified the mechanisms for Lactobacillus adhesion and pathogenic inhibition, providing valuable information for the development of new probiotics and the discovery of novel therapeutic targets for the prevention of infections in dairy cows.Chunyu NiuChao ChengYanmin LiuShaolei HuangYanru FuPeifeng LiAmerican Society for Microbiologyarticleprimary vaginal epithelial cell cultureLactobacillustranscriptome profilinggene expressionRT-qPCRMicrobiologyQR1-502ENmSystems, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	primary vaginal epithelial cell culture Lactobacillus transcriptome profiling gene expression RT-qPCR Microbiology QR1-502
spellingShingle	primary vaginal epithelial cell culture Lactobacillus transcriptome profiling gene expression RT-qPCR Microbiology QR1-502 Chunyu Niu Chao Cheng Yanmin Liu Shaolei Huang Yanru Fu Peifeng Li Transcriptome Profiling Analysis of Bovine Vaginal Epithelial Cell Response to an Isolated <italic toggle="yes">Lactobacillus</italic> Strain
description	ABSTRACT Lactobacillus strain SQ0048 isolated from bovine vagina has been shown to exhibit specific adherence to the epithelium and to produce inhibitory substances; however, the underlying mechanisms remain unclear. We cultured and identified primary bovine vaginal epithelial cells treated with SQ0048 to investigate the pathways involved in host cell responses using transcriptome sequencing (RNA-seq). Transcription profiling showed 296 significantly altered differentially expressed genes (DEGs), of which 170 were upregulated and 126 downregulated. Gene Ontology (GO) enrichment analysis of the DEGs revealed significant enrichment of 424 GO terms throughout the differentiation process (P < 0.05). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the DEGs were successfully annotated as members of 171 pathways, with 23 significantly enriched KEGG pathways (P < 0.05). A relatively high number of genes were enriched for the endoplasmic reticulum protein processing and interleukin-17 (IL-17) signaling pathways and for antigen processing and presentation. DEGs were verified by quantitative reverse transcription-PCR (RT-qPCR) and determination of which were most enriched for endoplasmic reticulum protein processing pathways, the activation of which might be a major factor underlying Lactobacillus adhesion to cells and pathogenic inhibition. IMPORTANCE Bovine bacterial vaginitis causes infertility, abortion, and postpartum uterine diseases, causing serious economic loss to the dairy industry. The large-scale use of antibiotics destroys normal genital tract flora and hinders the defense mechanisms of the host. Recent research suggests that lactobacilli present in the vaginal microflora of healthy cows constitute the primary microbiological barrier to infection by genital pathogens, exerting a protective role on the reproductive tract via specific adherence to the epithelium and the production of inhibitory substances. Our research identified the mechanisms for Lactobacillus adhesion and pathogenic inhibition, providing valuable information for the development of new probiotics and the discovery of novel therapeutic targets for the prevention of infections in dairy cows.
format	article
author	Chunyu Niu Chao Cheng Yanmin Liu Shaolei Huang Yanru Fu Peifeng Li
author_facet	Chunyu Niu Chao Cheng Yanmin Liu Shaolei Huang Yanru Fu Peifeng Li
author_sort	Chunyu Niu
title	Transcriptome Profiling Analysis of Bovine Vaginal Epithelial Cell Response to an Isolated <italic toggle="yes">Lactobacillus</italic> Strain
title_short	Transcriptome Profiling Analysis of Bovine Vaginal Epithelial Cell Response to an Isolated <italic toggle="yes">Lactobacillus</italic> Strain
title_full	Transcriptome Profiling Analysis of Bovine Vaginal Epithelial Cell Response to an Isolated <italic toggle="yes">Lactobacillus</italic> Strain
title_fullStr	Transcriptome Profiling Analysis of Bovine Vaginal Epithelial Cell Response to an Isolated <italic toggle="yes">Lactobacillus</italic> Strain
title_full_unstemmed	Transcriptome Profiling Analysis of Bovine Vaginal Epithelial Cell Response to an Isolated <italic toggle="yes">Lactobacillus</italic> Strain
title_sort	transcriptome profiling analysis of bovine vaginal epithelial cell response to an isolated <italic toggle="yes">lactobacillus</italic> strain
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/ec9e73ff35f64ba696bf4599cc295b7a
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Transcriptome Profiling Analysis of Bovine Vaginal Epithelial Cell Response to an Isolated <italic toggle="yes">Lactobacillus</italic> Strain

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